Cellular uptake and subcellular localization of highly luminescent silica-coated CdSe quantum dots – In vitro and in vivo

Vibin, M.; Vinayakan, R.; John, Annie; Rejiya, C.S.; Vijayamma, Raji; Abraham, Annie

doi:10.1016/j.jcis.2011.02.008

Cited by 15 publications

(7 citation statements)

References 32 publications

(42 reference statements)

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“…Trioctylphosphine oxide capped CdSe QDs were synthesised and made water soluble by silanisation. The details of QDs synthesis, silanisation and the evaluation of colloidal properties of QDs have been published elsewhere in detail [ 29 ]. The silica-coated CdSe QDs obtained showed a greenish yellow emission in PBS buffer (pH 7.3).…”

Section: Resultsmentioning

confidence: 99%

“…But many aspects of this approach need to be further optimized particularly for in vivo applications. Our preliminary in vitro investigations proved the cytocompatibility and stability of silica-coated CdSe QDs under biological conditions [ 29 ]. The objective of this study was to demonstrate that the silica-coated CdSe QDs can be used as labelling agents for long-life cellular imaging, cancer cellular imaging using cLSM and fluorescence microscopy.…”

Section: Introductionmentioning

confidence: 99%

“…By time and concentration dependent studies we observed that the internalized silica-coated CdSe QDs were non-toxic proving the cytocompatibility even at higher concentrations and longer incubation periods. We have also reported the non-specific cellular uptake and subcellular localization of silica-coated CdSe quantum dots [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Effective cellular internalization of silica-coated CdSe quantum dots for high contrast cancer imaging and labelling applications

et al. 2014

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The possibility of developing novel contrast imaging agents for cancer cellular labelling and fluorescence imaging applications were explored using silica-coated cadmium selenide (CdSe) quantum dots (QDs). The time dependent cellular internalization efficiency study was carried out using Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) and Confocal Laser Scanning Microscopy (cLSM) after exposing QDs to stem cells and cancer cells. The strong fluorescence from the cytoplasm confirmed that the QDs were efficiently internalized by the cells. The internalization maxima were observed at the fourth hour of incubation in both stem and cancer cells. Further, the in vitro fluorescence imaging as well as localization study of QDs were performed in various cells. Moreover, high contrast in vivo tumor imaging efficiency of silica-coated CdSe QDs was performed in ultrathin sections of tumor mice, and the results confirmed its effective role in cellular imaging and labelling in cancer and other diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effective cellular internalization of silica-coated CdSe quantum dots for high contrast cancer imaging and labelling applications

et al. 2014

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“…For gene therapy, negatively charged genetic molecules are able to complex with amine-modified SiNPs with a positive charge, 103 either on the surface or inside the pores. In addition, the conjugation/hybridation of silica materials with polymers 39 or other inorganic materials 104,105 will acquire some new properties. For protein therapeutics, different strategies are required to achieve protein loading, due to the variable sizes and isoelectric points (IEPs) of protein molecules.…”

Section: Surface Engineeringmentioning

confidence: 99%

“…197 Nel et al 198 depicted the effect of heterogeneous structures, for example, local protrusions or depressions with radii smaller than that of the particle, may have the potential to strengthen NPcell interactions. Although some silica-based bimodal or heterogeneous structures have been fabricated, for example, the raspberry-like structure, 84,100,104 that is, the combination of a large core and plenty of small shells on the surface, only a few studies have focused on the relationship between topology and gene/protein delivery efficiency.…”

Section: Particle Sizementioning

confidence: 99%

Bio-inspired virus-mimicking nanoparticles for efficient cellular delivery

Niu¹

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Delivery of various drugs and biomolecules into cells is crucial in modern medicine, providing promising potential in the treatment of incurable diseases. Most naked therapeutic biomolecules, for example, proteins, siRNAs and some free drugs can hardly penetrate into cells, thus various natural particulates and synthetic vectors have been used as cellular delivery vehicles. The understanding of structure-function relationships of natural particulates provides a useful guide for the design of new nanocarriers with better safety and higher delivery efficiency. Currently, many research attempts have focused on the synthesis of new drug delivery systems by mimicking the advantages of enveloped viruses, which have evolved sophisticated mechanisms that make use of or shield off cellular signalling and transport pathways to traffic within host cells and deliver cargos into appropriate subcellular compartments. However, there are still some parameters of enveloped viruses requiring intensive study, for example, the contribution of viral surface topography (rough surface) to intracellular delivery of cargo molecules.This thesis focuses on the development of a novel drug delivery system with high performance based on the preparation of silica nanoparticles with virus-mimicking rough morphology and gains insight into the roles of surface roughness variation and surface functionality (e.g. polyethylenimine and octadecyl-group) in biomolecule (e.g. siRNAs and therapeutic proteins) delivery performance.The main achievements obtained in this thesis are listed below.In the first part, a new and facile approach has been developed to prepare the virus-mimicking silica nanoparticle (VMSN) with a rough surface. We show that increases in nanoscale surface roughness promote both binding of biomolecules (e.g., genetic molecules) and cellular uptake; thus, the cargo delivery efficiency is significantly increased, regardless of surface functionality and cell types.Finally, gene delivery efficiency was tested, where the biomimetic nanoparticles shows a better cell growth inhibition performance than the smooth silica nanoparticle and a commercial delivery reagent.In the second part, the novel and facile approach for systematically controlling surface roughness of silica nanoparticles has been developed. Based on our "neck-enhancing" approach, rough silica nanoparticles (RSNs) with a fixed core particle (211 nm in diameter) and varied shell particles are obtained. The increase of shell particle s z s rom 13 to 98 nm enlarges interspacing distance tw n n our n s ll p rt l s rom 7 to 38 nm, where protein molecules will favourably absorb onto one of RSNs without impacting protein binding ability. Publications included in this thesis1. Yuting Niu, Amirali Popat, Meihua Yu, Surajit Karmakar, Wenyi Gu and Chengzhong Yu.Recent advances in the rational design of silica-based nanoparticles for gene therapy.

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Highly Selective and Stable Florescent Sensor for Cd(II) Based on Poly(azomethine-urethane)

Kaya

Kamacı

2012

J Fluoresc

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In this study a kind of poly(azomethine-urethane); (E)-4-((2 hydroxyphenylimino) methyl)-2-methoxyphenyl 6-acetamidohexylcarbamate (HDI-co-3-DHB-2-AP) was prepared as in the literature and employed as a new fluorescent probe for detection of Cd(II) concentration. The photoluminescence (PL) measurements were carried out in the presence of several kinds of heavy metals. HDI-co-3-DHB-2-AP gave a linearly and highly stable response against Cd(II) as decreasing a new emission peak at 562 nm. Possible interferences of other ions were found too low. Detection limit of the sensor was found as 8.86 × 10(-4) mol L(-1). Resultantly, HDI-co-3- DHB-2-AP could be effectively used as an optical Cd(II) sensor.

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Cellular uptake and subcellular localization of highly luminescent silica-coated CdSe quantum dots – In vitro and in vivo

Cited by 15 publications

References 32 publications

Effective cellular internalization of silica-coated CdSe quantum dots for high contrast cancer imaging and labelling applications

Effective cellular internalization of silica-coated CdSe quantum dots for high contrast cancer imaging and labelling applications

Bio-inspired virus-mimicking nanoparticles for efficient cellular delivery

Highly Selective and Stable Florescent Sensor for Cd(II) Based on Poly(azomethine-urethane)

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