Cellular uptake and activity of heparin functionalised cerium oxide nanoparticles in monocytes

Ting, S. R. Simon; Whitelock, John M.; Tomic, Romana; Gunawan, Cindy; Teoh, Wey Yang; Amal, Rose; Lord, Megan S.

doi:10.1016/j.biomaterials.2013.02.042

Cited by 54 publications

(44 citation statements)

References 46 publications

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“…The proliferation of THP-1 after incubation with eENP is associated with an upregulation of FGFR1. In the same way, Ting et al (2013) 22 have observed that heparin-nanoceria formed active ternary complexes with FGF2 and FGFR1 inducing BaF32 myeloid B cell proliferation. An activation of genes involved in histone rearrangement as observed with eENP is in favor of an alteration of the cell cycle as it has been reported for human aortic endothelial cells in which poly(amido amine) dendrimers and silica nanoparticles induce a modification of many histone gene expressions.…”

Section: Discussionmentioning

confidence: 86%

Human Monocyte Response to S-Nitrosoglutathione-Loaded Nanoparticles: Uptake, Viability, and Transcriptome

et al. 2015

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S-Nitrosoglutathione (GSNO) is a good candidate for nitric oxide (NO(•)) delivery, and its nanoformulation improves NO(•) stability and bioavailability. We have compared the effect of empty Eudragit nanoparticles (eENP), GSNO-loaded ENP (gENP), and free GSNO on THP-1 human monocytic cell line. We investigated cellular viability and growth by WST-1 and trypan blue tests. ENP uptake was studied using transmission electron microscopy, confocal microscopy, and flow cytometry. Transcriptomic profiles were obtained using microarray. ENP entered cells by clathrin- and caveolae-mediated endocytosis. Exposure to either free GSNO or gENP induced an activation of genes from the same clusters, in favor of intracellular delivery of GSNO by ENP. GSNO nanoformulation might be a therapeutic option for NO(•) delivery.

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Section: Discussionmentioning

confidence: 86%

Human Monocyte Response to S-Nitrosoglutathione-Loaded Nanoparticles: Uptake, Viability, and Transcriptome

et al. 2015

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“…Indeed, the choice of stabilizer and the resulting chemical characteristics did impact uptake by non-immune cell lines in several studies 121, 143 . Increased cellular uptake of heparin-functionalized nanoceria compared to non-stabilized nanoceria by a human monocyte cell line is some evidence that stabilizer choice does in fact affect immune cell responses 144 . However, regardless of stabilizer coating, the nanoceria was able to reduce ROS levels in this activated monocyte cell line 144, 145 .…”

Section: Traversing Physiological Barriers: Differences In Biologmentioning

confidence: 99%

“…Increased cellular uptake of heparin-functionalized nanoceria compared to non-stabilized nanoceria by a human monocyte cell line is some evidence that stabilizer choice does in fact affect immune cell responses 144 . However, regardless of stabilizer coating, the nanoceria was able to reduce ROS levels in this activated monocyte cell line 144, 145 . Decreased ROS production was also observed in two murine macrophage cell lines that were either unstimulated 146 or stimulated 117 .…”

Section: Traversing Physiological Barriers: Differences In Biologmentioning

confidence: 99%

Catalytic properties and biomedical applications of cerium oxide nanoparticles

Walkey

Das

Seal

et al. 2015

Environ. Sci.: Nano

314

233

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Cerium oxide nanoparticles (Nanoceria) have shown promise as catalytic antioxidants in the test tube, cell culture models and animal models of disease. However given the reactivity that is well established at the surface of these nanoparticles, the biological utilization of Nanoceria as a therapeutic still poses many challenges. Moreover the form that these particles take in a biological environment, such as the changes that can occur due to a protein corona, are not well established. This review aims to summarize the existing literature on biological use of Nanoceria, and to raise questions about what further study is needed to apply this interesting catalytic material to biomedical applications. These questions include: 1) How does preparation, exposure dose, route and experimental model influence the reported effects of Nanoceria in animal studies? 2) What are the considerations to develop Nanoceria as a therapeutic agent in regards to these parameters? 3) What biological targets of reactive oxygen species (ROS) and reactive nitrogen species (RNS) are relevant to this targeting, and how do these properties also influence the safety of these nanomaterials?

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“…It is also known that in the stress-induced cell response model, the heat-shocked proteins play a key role in early events. 16,17 However, the role of these NPs as cell scavengers through the induction of heat-shocked proteins and their genes in cellular stress is not fully characterized. Future experiments will be focused on determining the exact mechanisms of how ceria and selenium in this study were able to decrease fibroblast death due to heat shock.…”

mentioning

confidence: 99%

Cytoprotective effects of cerium and selenium nanoparticles on heat-shocked human dermal fibroblasts: an in vitro evaluation

Yuan

Webster

Roy

2016

IJN

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It is a widely accepted fact that environmental factors affect cells by modulating the components of subcellular compartments and altering metabolic enzymes. Factors (such as oxidative stress and heat-shock-induced proteins and heat shock factors, which upregulate stress-response related genes to protect affected cells) are commonly altered during changes in environmental conditions. Studies by our group and others have shown that nanoparticles (NPs) are able to efficiently attenuate oxidative stress by penetrating into specific tissues or organs. Such findings warrant further investigation on the effects of NPs on heat-shock-induced stress, specifically in cells in the presence or absence (pretreated) of NPs. Here, we examined the cytoprotective effects of two different NPs (cerium and selenium) on heat-induced cell death for a model cell using dermal fibroblasts. We report for the first time that both ceria and selenium NPs (at 500 µg/mL) possess stress-relieving behavior on fibroblasts undergoing heat shock. Such results indicate the need to further develop these NPs as a novel treatment for heat shock.

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Cellular uptake and activity of heparin functionalised cerium oxide nanoparticles in monocytes

Cited by 54 publications

References 46 publications

Human Monocyte Response to S-Nitrosoglutathione-Loaded Nanoparticles: Uptake, Viability, and Transcriptome

Human Monocyte Response to S-Nitrosoglutathione-Loaded Nanoparticles: Uptake, Viability, and Transcriptome

Catalytic properties and biomedical applications of cerium oxide nanoparticles

Cytoprotective effects of cerium and selenium nanoparticles on heat-shocked human dermal fibroblasts: an in vitro evaluation

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