Cellular Tensegrity: Exploring How Mechanical Changes in the Cytoskeleton Regulate Cell Growth, Migration, and Tissue Pattern during Morphogenesis

Ingber, Donald E.; Dike, Laura E.; Hansen, Linda K.; Karp, Seth J.; Liley, Helen; Maniotis, Andrew J.; McNamee, Helen P.; Mooney, David J.; Plopper, George E.; Sims, John R.; Wang, Ning

doi:10.1016/s0074-7696(08)61542-9

Cited by 391 publications

(219 citation statements)

References 184 publications

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“…The ECM has a pivotal role in regulating cellular function, acting as a substrate for cellular adhesion, migration, differentiation, proliferation and survival and as a scaffold. 15 Airway fibroblasts are localized beneath the BM and, when activated, become myofibroblasts, characterized by the expression of alpha smooth muscle actin (a-SMA), with rapid ECM production. 16 ECM turnover is orchestrated by the balance between the expression of matrix metalloproteinases (MMP) and their inhibitors (TIMP).…”

Section: Airway Structure In Healthmentioning

confidence: 99%

Upper and lower airway remodelling mechanisms in asthma, allergic rhinitis and chronic rhinosinusitis: The one airway concept revisited

Samitas

Carter

Kariyawasam

et al. 2017

Allergy

187

156

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Allergic rhinitis (AR), chronic rhinosinusitis (CRS) and asthma often co-exist. The one airway model proposes that disease mechanisms occurring in the upper airway may mirror lower airway events. Airway remodelling is the term used to describe tissue structural changes that occur in a disease setting and reflect the dynamic process of tissue restructuring during wound repair. Remodelling has been long identified in the lower airways in asthma and is characterized by epithelial shedding, goblet cell hyperplasia, basement membrane thickening, subepithelial fibrosis, airway smooth muscle hyperplasia and increased angiogenesis. The concept of upper airway remodelling has only recently been introduced, and data so far are limited and often conflicting, an indication that more detailed studies are needed. Whilst remodelling changes in AR are limited, CRS phenotypes demonstrate epithelial hyperplasia, increased matrix deposition and degradation along with accumulation of plasma proteins. Despite extensive research over the past years, the precise cellular and molecular mechanisms involved in airway remodelling remain incompletely defined. This review describes our current rather limited understanding of airway remodelling processes in AR, CRS and asthma and presents mechanisms both shared and distinct between the upper and lower airways. Delineation of shared and disease-specific pathogenic mechanisms of remodelling between the sinonasal system and the lung may guide the rational design of more effective therapeutic strategies targeting upper and lower airways concomitantly and improving the health of individuals with inflammatory airway diseases.

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Section: Airway Structure In Healthmentioning

confidence: 99%

Upper and lower airway remodelling mechanisms in asthma, allergic rhinitis and chronic rhinosinusitis: The one airway concept revisited

Samitas

Carter

Kariyawasam

et al. 2017

Allergy

187

156

View full text Add to dashboard Cite

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“…30 Thus, the effect of ECM on cell adhesion and shape might result in cytoskeletal/nuclear skeletal configurations which do not allow proliferation. 5,63 On the other hand, other ECM molecules encode signals which can modulate proliferation in a growth factorlike manner in the absence of gross changes in cellular morphology. [64][65][66][67] It is tempting to speculate that when the basement membrane is broken down by the proteolysis in tumors, the released fragments may feed back on the behavior of the cells both locally and through circulation.…”

Section: Proliferationmentioning

confidence: 99%

“…3 The precise mode of ECM action remains elusive, but available evidence suggests that ECM influences cellular behavior by both changes of the three-dimensional organization of the cytoskeleton and activation of second messenger and protein kinase pathways. [4][5][6] It is in fact conceivable that some of the known oncogenes and tumor suppressor genes interfere with intracellular pathways conveying ECM signals. [7][8][9] When normal mammary epithelial cells or cell lines from rodents or humans are placed in culture and maintained in the absence of ECM, they spread, proliferate and grow as a poorly polarized monolayer.…”

mentioning

confidence: 99%

Involvement of extracellular matrix constituents in breast cancer

Lochter

Bissell

1995

Seminars in Cancer Biology

164

136

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“…In recent years, the importance of the mechanical characterization of biological materials has become evident in the field of mechanobiology, ranging from the diagnosis of diseases like cancer 1 and malaria 2 to the understanding of mechanisms in cell biology such as cell growth 3 and locomotion. 4,5 Biological materials are commonly soft and inhomogeneous in nature, which can make them difficult to characterize using traditional mechanical experiments.…”

Section: Introductionmentioning

confidence: 99%

Analysis of nanoindentation of soft materials with an atomic force microscope

2011

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Nanoindentation is a popular experimental technique for characterization of the mechanical properties of soft and biological materials. With its force resolution of tens of pico-Newtons, the atomic force microscope (AFM) is well-suited for performing indentation experiments on soft materials. However, nonlinear contact and adhesion complicate such experiments. This paper critically examines the application of the Johnson-Kendall-Roberts (JKR) adhesion model to nanoindentation data collected with an AFM. The use of a nonlinear least-square error-fitting algorithm to calculate reduced modulus from the nanoindentation data using the JKR model is discussed. It is found that the JKR model fits the data during loading but does not fit the data during unloading. A fracture stability analysis shows that the JKR model does not fit the data collected during unloading because of the increased stability provided by the AFM cantilever.

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Cellular Tensegrity: Exploring How Mechanical Changes in the Cytoskeleton Regulate Cell Growth, Migration, and Tissue Pattern during Morphogenesis

Cited by 391 publications

References 184 publications

Upper and lower airway remodelling mechanisms in asthma, allergic rhinitis and chronic rhinosinusitis: The one airway concept revisited

Upper and lower airway remodelling mechanisms in asthma, allergic rhinitis and chronic rhinosinusitis: The one airway concept revisited

Involvement of extracellular matrix constituents in breast cancer

Analysis of nanoindentation of soft materials with an atomic force microscope

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