Cellular signaling by neural cell adhesion molecules of the immunoglobulin superfamily

Crossin, Kathryn L.; Krushel, Leslie A.

doi:10.1002/(sici)1097-0177(200006)218:2<260::aid-dvdy3>3.0.co;2-9

Cited by 238 publications

(161 citation statements)

References 284 publications

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“…Therefore, at least, two receptor signaling pathways, c-Ret and NCAM activated, exist in SSCs. Although NCAM is a homophilic and heterophilic adhesion molecule, it is involved in various biological functions (26). Therefore, NCAM may have more than one role in SSC biology.…”

Section: Discussionmentioning

confidence: 99%

Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat

Ryu

Kubota

Avarbock

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

251

236

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Self-renewal of spermatogonial stem cells (SSCs) is the foundation for maintenance of spermatogenesis throughout life in males and for continuation of a species. The molecular mechanism underlying stem cell self-renewal is a fundamental question in stem cell biology. Recently, we identified growth factors necessary for self-renewal of mouse SSCs and established a serum-free culture system for their proliferation in vitro. To determine whether the stimulatory signals for SSC replication are conserved among different species, we extended the culture system to rat SSCs. Initially, a method to assess in vitro expansion of SSCs was developed by using flow cytometric analysis, and, subsequently, we found that a combination of glial cell line-derived neurotrophic factor, soluble glial cell line-derived neurotrophic factor-family receptor ␣-1 and basic fibroblast growth factor supports proliferation of rat SSCs. When cultured with the three factors, stem cells proliferated continuously for >7 months, and transplantation of the cultured SSCs to recipient rats generated donor stem cell-derived progeny, demonstrating that the cultured stem cells are normal. The growth factor requirement for replication of rat SSCs is identical to that of mouse; therefore, the signaling factors for SSC self-renewal are conserved in these two species. Because SSCs from many mammals, including human, can replicate in mouse seminiferous tubules after transplantation, the growth factors required for SSC self-renewal may be conserved among many different species. Furthermore, development of a long-term culture system for rat SSCs has established a foundation for germ-line modification of the rat by gene targeting technology.germ-line stem cell ͉ spermatogenesis ͉ testis ͉ glial cell line-derived neurotrophic factor

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Section: Discussionmentioning

confidence: 99%

Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat

Ryu

Kubota

Avarbock

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

251

236

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“…2D), yet targeting of neuroligin to synapses is independent of this binding, supporting suggestions that neuroligin is recruited early in synaptogenesis . Labeling for Nr-CAM, a member of the L1 family of cell adhesion molecules (Crossin and Krushel, 2000;Hortsch, 2000), often was seen in clusters of gold particles in the synaptic cleft, suggesting that the antibodies label molecules linked together across the synaptic cleft. Previous studies have indicated that both Nr-CAM and L1 are presynaptic (Lustig et al, 2001;Matsumoto-Miyai et al, 2003), and these proteins commonly form homophilic bonds between cell processes (Hortsch, 2000;Alberts and Galli, 2003).…”

Section: Discussionmentioning

confidence: 99%

Ontogeny of postsynaptic density proteins at glutamatergic synapses

Petralia

Sans

Wang

et al. 2005

Molecular and Cellular Neuroscience

206

196

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In glutamatergic synapses, glutamate receptors (GluRs) associate with many other proteins involved in scaffolding and signal transduction. The ontogeny of these postsynaptic density (PSD) proteins involves changes in their composition during development, paralleling changes in GluR type and function. In the CA1 region of the hippocampus, at postnatal day 2 (P2), many synapses already have a distinct PSD. We used immunoblot analysis, subcellular fractionation, and quantitative immunogold electron microscopy to examine the distribution of PSD proteins during development of the hippocampus. Synapses at P2 contained substantial levels of NR1 and NR2B and most GluRassociated proteins, including SAP102, SynGAP, the chain of proteins from GluRs/SAP102 through GKAP/Shank/Homer and metabotropic glutamate receptors, and the adhesion factors, cadherin, catenin, neuroligin, and Nr-CAM. Development was marked by substantial decreases in NR2B and SAP102 and increases in NR2A, PSD-95, AMPA receptors and CaMKII. Other components showed more moderate changes.

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“…N-CAM influences cell proliferation, differentiation, migration, neurite outgrowth, and fasciculation during development and in the adult (8). Our previous studies indicated that N-CAM binding inhibited proliferation of rat hippocampal progenitor cells (9) in culture similar to its effect on astrocytes (10,11).…”

mentioning

confidence: 90%

Cultured rat hippocampal neural progenitors generate spontaneously active neural networks

Mistry

Keefer

Cunningham

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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We previously demonstrated that the neural cell adhesion molecule (N-CAM) inhibited the proliferation of cultured rat hippocampal progenitor cells and increased the number of neurons generated. We demonstrate here that the continued presence of fibroblast growth factor 2 along with N-CAM or brain-derived neurotrophic factor over 12 days of culture greatly increased the number of both progenitors and neurons. These progenitorderived neurons expressed neurotransmitters, neurotransmitter receptors, and synaptic proteins in vitro consistent with those expressed in the mature hippocampus. Progenitor cells cultured on microelectrode plates formed elaborate neural networks that exhibited spontaneously generated action potentials after 21 days. This activity was observed only in cultures grown in the presence of fibroblast growth factor 2 and either N-CAM or brain-derived neurotrophic factor. Analysis of neuronal activity after various pharmacological treatments indicated that the networks formed functional GABAergic and glutamatergic synapses. We conclude that mitogenic growth factors can synergize with N-CAM or neurotrophins to generate spontaneously active neural networks from neural progenitors.

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Cellular signaling by neural cell adhesion molecules of the immunoglobulin superfamily

Cited by 238 publications

References 284 publications

Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat

Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat

Ontogeny of postsynaptic density proteins at glutamatergic synapses

Cultured rat hippocampal neural progenitors generate spontaneously active neural networks

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