Cellular senescence and autophagy of myoepithelial cells are involved in the progression of in situ areas of carcinoma ex-pleomorphic adenoma to invasive carcinoma. An in vitro model

Silva, Carolina Amália Barcellos; Martinez, Elizabeth Ferreira; Demasi, Ana Paula Dias; Altemani, Albina; Bossonaro, Jeruza P.; Araújo, Ney Soares de; Araújo, Vera Cavalcanti de

doi:10.1007/s12079-015-0291-9

Cited by 12 publications

(16 citation statements)

References 44 publications

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“…These findings corroborate a previous study by Silva et al, 4 who demonstrated that Myo cell disappearance from areas of invasive carcinoma in situ was not associated with apoptosis but with an induction of senescence and autophagy when Myo cells were co‐cultured with malignant epithelial cells. This could explain the loss observed in Myo cell population during the progression of ex‐pleomorphic adenoma carcinoma 5,6 .…”

Section: Discussionsupporting

confidence: 92%

“…These findings suggest that MV produced by malignant epithelial cells may play an important role in the transition from in situ to invasive carcinoma in cases of carcinomas ex‐pleomorphic adenoma (CXAP). During the progression of this neoplasia, Myo cells disappear, 5,6 and recent evidence has suggested that these cells may undergo autophagy‐senescence 4 …”

Section: Discussionmentioning

confidence: 99%

“…In this model, myoepithelial (Myo) cells derived from pleomorphic adenoma are co‐cultivated with malignant neoplastic epithelial cells, thus permitting specific analysis on how reciprocal inductions can affect cell phenotype. Indeed, Silva et al 4 observed an induction of senescence and autophagy processes in Myo cells mediated by malignant epithelial cells, which could explain the reduction in the former cell population during the progression of carcinomas ex‐pleomorphic adenoma 5,6 …”

Section: Introductionmentioning

confidence: 99%

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Microvesicles derived from squamous cell carcinoma induce cell death, autophagy, and invasion of benign myoepithelial cells

Martinez

Araújo

Navarini

et al. 2020

J Oral Pathology Medicine

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Background There has been great interest recently in the mechanisms of cell‐to‐cell communication through microvesicles (MV). These structures are produced by many different cell types and can modulate cellular activity by induction of epigenetic alterations. These vesicles may promote tumor mass increase either by stimulating cell proliferation via growth factors or by inhibiting apoptosis, which reinforces the role of such vesicles as important modulators of tumor progression. Methods The present in vitro study aimed to characterize MV derived from malignant neoplastic epithelial cell cultures (EP) and their effect on the expression of apoptosis/autophagy and invasion related genes of benign myoepithelial (Myo) cell cultures. Results The results revealed round structures with a mean size of 153.6 (±0.2) nm, with typical MV morphology. CD63 quantification indicated that EP cell culture at 70%‐80% confluence secreted 3.088 × 108 MV/mL. Overall, Myo exposed to MVs derived from EP showed both up‐ and downregulation of tumorigenesis promoting genes. MVs from EP cells promoted cell death of Myo cells and positively modulate BAX, SURVIVIN, LC3B, MMP‐2, and MMP‐9 expression. Furthermore, an increasing of MMP‐2 and MMP‐9 secretion by Myo was observed after MV exposure. Conclusions These findings suggest that MVs from EP modulate autophagy of Myo cells, which may, in part, explain the disappearance of these cells in in situ areas of invasive carcinoma ex‐pleomorphic adenoma. Additionally, the overexpression of MMPs contributes to the development of an invasive phenotype of Myo cells, which could favor the dissolution of the basement membrane during tumorigenesis process.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microvesicles derived from squamous cell carcinoma induce cell death, autophagy, and invasion of benign myoepithelial cells

Martinez

Araújo

Navarini

et al. 2020

J Oral Pathology Medicine

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“…As for cnidarian epitheliomuscular cells, bilaterians myoepithelial cells originate from tissues of various developmental origins (Petersen and van Deurs, 1989; Schmidt-Rhaesa, 2007; Tamgadge et al, 2013). Furthermore, myoepithelial cells in mammalians are receiving increasing interest, because of their importance in processes such as gland development, growth and differentiation, in pathologies such as breast cancer (Silva et al, 2015) as well as their capacity to control tumorigenesis (Gudjonsson et al, 2005; reviewed in Deugnier et al, 2002; Sopel, 2010). Thus, it could be particularly fruitful to compare bilaterian myoepithelial and cnidarian epitheliomuscular cells, their developmental origin, the molecular or mechanical signals that control their polarization, condensation and organization into muscle nets, rings or fibers and how they regenerate after injury.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Cnidarian Muscles: Function, Anatomy, Development and Regeneration

Leclère

Röttinger

2017

Front. Cell Dev. Biol.

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The ability to perform muscle contractions is one of the most important and distinctive features of eumetazoans. As the sister group to bilaterians, cnidarians (sea anemones, corals, jellyfish, and hydroids) hold an informative phylogenetic position for understanding muscle evolution. Here, we review current knowledge on muscle function, diversity, development, regeneration and evolution in cnidarians. Cnidarian muscles are involved in various activities, such as feeding, escape, locomotion and defense, in close association with the nervous system. This variety is reflected in the large diversity of muscle organizations found in Cnidaria. Smooth epithelial muscle is thought to be the most common type, and is inferred to be the ancestral muscle type for Cnidaria, while striated muscle fibers and non-epithelial myocytes would have been convergently acquired within Cnidaria. Current knowledge of cnidarian muscle development and its regeneration is limited. While orthologs of myogenic regulatory factors such as MyoD have yet to be found in cnidarian genomes, striated muscle formation potentially involves well-conserved myogenic genes, such as twist and mef2. Although satellite cells have yet to be identified in cnidarians, muscle plasticity (e.g., de- and re-differentiation, fiber repolarization) in a regenerative context and its potential role during regeneration has started to be addressed in a few cnidarian systems. The development of novel tools to study those organisms has created new opportunities to investigate in depth the development and regeneration of cnidarian muscle cells and how they contribute to the regenerative process.

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“…However, this condition is short-lived, as myoepithelial cells rapidly disappear (8,9). A number of hydrolytic enzymes, released either by the tumor cells or by cells surrounding the tumor, combined with certain growth factors may favor disruption of the basement membrane, contributing to EMT (10).…”

Section: Introductionmentioning

confidence: 99%

Effect of epithelial growth factor on matrix metalloproteinase-2 and E-cadherin/β-catenin expression in an in situ model of tumorigenesis

et al. 2017

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Abstract. The aim of the present study was to analyze the in vitro effect of various doses of epidermal growth factor (EGF; 5 and 10 ng/ml) on matrix metalloproteinase-2 (MMP-2) secretion and E-cadherin/β-catenin expression by co-cultured cells that mimic an in situ carcinoma ex-pleomorphic adenoma, where benign myoepithelial cells from a pleomorphic adenoma surround malignant epithelial cells. EGF was supplemented in various doses and the effects were evaluated following four days of cell culture. ELISA was performed to determine MMP-2 secretion levels. Gene expression for E-cadherin and β-catenin was analyzed using quantitative polymerase chain reaction. The results revealed that E-cadherin expression decreased when the cells were supplemented with 5 ng/ml EGF. ELISA results indicated that MMP-2 secretion increased when EGF was supplemented at concentrations of 5 and 10 ng/ml. The present findings demonstrated that EGF may be involved in the epithelial-mesenchymal transition process via altering the E-cadherin/β-catenin complex and increasing MMP-2 secretion, which may then favor the dissolution of the basement membrane to the benefit of malignant cell clusters, contributing to the development of an invasive phenotype in this in vitro model of tumorigenesis.

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Cellular senescence and autophagy of myoepithelial cells are involved in the progression of in situ areas of carcinoma ex-pleomorphic adenoma to invasive carcinoma. An in vitro model

Cited by 12 publications

References 44 publications

Microvesicles derived from squamous cell carcinoma induce cell death, autophagy, and invasion of benign myoepithelial cells

Microvesicles derived from squamous cell carcinoma induce cell death, autophagy, and invasion of benign myoepithelial cells

Diversity of Cnidarian Muscles: Function, Anatomy, Development and Regeneration

Effect of epithelial growth factor on matrix metalloproteinase-2 and E-cadherin/β-catenin expression in an in situ model of tumorigenesis

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