Cellular responses to environmental contaminants in amoebic cells of the slime mould Dictyostelium discoideum

Dondero, Francesco; Jönsson, Henrik; Rebelo, Mauro de Freitas; Pesce, Gabriella; Berti, Elena; Pons, G.; Viarengo, Aldo

doi:10.1016/j.cbpc.2006.01.005

Cited by 17 publications

(37 citation statements)

References 25 publications

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“…Lysosomal membrane destabilization constitutes a general stress response (biomarker), which might ultimately result in severe cell damage and even death ; this might explain the parallel found between cell viability and lysosomal membrane stability. The absence of significant differences between treatments in cell growth cannot be attributed to the absence of cellular replication because the number of cell doubled within 24 h, as previously reported under similar experimental conditions . It has been reported that this chronic (24‐h) response is less sensitive to pollutant exposure than acute responses such as cell viability, lysosomal membrane stability, and phagocytosis rate .…”

Section: Discussionsupporting

confidence: 63%

Toxicity assessment of diesel‐ and metal‐contaminated soils through elutriate and solid phase assays with the slime mold Dictyostelium discoideum

Rodríguez-Ruiz

Dondero

Viarengo

et al. 2016

Enviro Toxic and Chemistry

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A suite of organisms from different taxonomical and ecological positions is needed to assess environmentally relevant soil toxicity. A new bioassay based on Dictyostelium is presented that is aimed at integrating slime molds into such a testing framework. Toxicity tests on elutriates and the solid phase developmental cycle assay were successfully applied to a soil spiked with a mixture of Zn, Cd, and diesel fuel freshly prepared (recently contaminated) and after 2 yr of aging. The elutriates of both soils provoked toxic effects, but toxicity was markedly lower in the aged soil. In the D. discoideum developmental cycle assay, both soils affected amoeba viability and aggregation, with fewer multicellular units, smaller fruiting bodies and, overall, inhibition of fruiting body formation. This assay is quick and requires small amounts of test soil, which might facilitate its incorporation into a multispecies multiple-endpoint toxicity bioassay battery suitable for environmental risk assessment in soils. Environ Toxicol Chem 2016;35:1413-1421. © 2015 SETAC.

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Section: Discussionsupporting

confidence: 63%

Toxicity assessment of diesel‐ and metal‐contaminated soils through elutriate and solid phase assays with the slime mold Dictyostelium discoideum

Rodríguez-Ruiz

Dondero

Viarengo

et al. 2016

Enviro Toxic and Chemistry

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“…Curiously, at the highest Hg concentrations in our study, lysosomal membrane destabilization likely induced the acidification of chambers early in the experiment (T1-100 ppm; Fig 2C) whereas the very low levels of lysosomal activity manifest as AO MFI that occurred at the end of the experiment (T2-100 ppm) may have been due to specimen death. Pollutants are known to destabilize the membranes of lysosomes causing the release of hydrolytic enzymes [59], as reported in eukaryotes [60] including protists [61]. …”

Section: Discussionmentioning

confidence: 99%

Mercury-Pollution Induction of Intracellular Lipid Accumulation and Lysosomal Compartment Amplification in the Benthic Foraminifer Ammonia parkinsoniana

et al. 2016

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Heavy metals such as mercury (Hg) pose a significant health hazard through bioaccumulation and biomagnification. By penetrating cell membranes, heavy metal ions may lead to pathological conditions. Here we examined the responses of Ammonia parkinsoniana, a benthic foraminiferan, to different concentrations of Hg in the artificial sea water. Confocal images of untreated and treated specimens using fluorescent probes (Nile Red and Acridine Orange) provided an opportunity for visualizing the intracellular lipid accumulation and acidic compartment regulation. With increased Hg over time, we observed an increased number of lipid droplets, which may have acted as a detoxifying organelle where Hg is sequestered and biologically inactivated. Further, Hg seems to promote the proliferation of lysosomes both in terms of number and dimension that, at the highest level of Hg, resulted in cell death. We report, for the first time, the presence of Hg within the foraminiferal cell: at the basal part of pores, in the organic linings of the foramen/septa, and as cytoplasmic accumulations.

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“…To synchronize the cell cycle, the amoebae in logarithmic phase (2–4 × 10 6 cells/mL) were stored overnight at 4 °C [76]. The cell treatment was performed in diluted medium for 6 h as described previously [77]. Chemicals of analytical grade were purchased from Sigma-Aldrich if not stated otherwise.…”

Section: Methodsmentioning

confidence: 99%

“…Biomarker assays were performed as described previously [77]. Briefly, the mortality rate was assessed using the DNA-binding dye SYBR Green™ (Sigma-Aldrich, Milan, Italy), and observations of the cells were performed at 460× magnification on a Zeiss Axiovert 100M.…”

Section: Methodsmentioning

confidence: 99%

Effects of Nickel, Chlorpyrifos and Their Mixture on the Dictyostelium discoideum Proteome

Boatti

Robotti

Marengo

et al. 2012

IJMS

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Mixtures of chemicals can have additive, synergistic or antagonistic interactions. We investigated the effects of the exposure to nickel, the organophosphate insecticide chlorpyrifos at effect concentrations (EC) of 25% and 50% and their binary mixture (Ec25 + EC25) on Dictyostelium discoideum amoebae based on lysosomal membrane stability (LMS). We treated D. discoideum with these compounds under controlled laboratory conditions and evaluated the changes in protein levels using a two-dimensional gel electrophoresis (2DE) proteomic approach. Nickel treatment at EC25 induced changes in 14 protein spots, 12 of which were down-regulated. Treatment with nickel at EC50 resulted in changes in 15 spots, 10 of which were down-regulated. Treatment with chlorpyrifos at EC25 induced changes in six spots, all of which were down-regulated; treatment with chlorpyrifos at EC50 induced changes in 13 spots, five of which were down-regulated. The mixture corresponding to EC25 of each compound induced changes in 19 spots, 13 of which were down-regulated. The data together reveal that a different protein expression signature exists for each treatment, and that only a few proteins are modulated in multiple different treatments. For a simple binary mixture, the proteomic response does not allow for the identification of each toxicant. The protein spots that showed significant differences were identified by mass spectrometry, which revealed modulations of proteins involved in metal detoxification, stress adaptation, the oxidative stress response and other cellular processes.

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Cellular responses to environmental contaminants in amoebic cells of the slime mould Dictyostelium discoideum

Cited by 17 publications

References 25 publications

Toxicity assessment of diesel‐ and metal‐contaminated soils through elutriate and solid phase assays with the slime mold Dictyostelium discoideum

Toxicity assessment of diesel‐ and metal‐contaminated soils through elutriate and solid phase assays with the slime mold Dictyostelium discoideum

Mercury-Pollution Induction of Intracellular Lipid Accumulation and Lysosomal Compartment Amplification in the Benthic Foraminifer Ammonia parkinsoniana

Effects of Nickel, Chlorpyrifos and Their Mixture on the Dictyostelium discoideum Proteome

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