Cellular responses to enteropathogenic<i>Escherichia coli</i>infection

Knutton, Stuart

doi:10.1007/bf01204350

Cited by 13 publications

(5 citation statements)

References 43 publications

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“…Dramatic loss of microvilli and the subsequent malabsorption due to brush border enzyme deficiency certainly contributes to diarrhoea. However, this alone is insufficient to account for the rapid onset of diarrhoea reported in human volunteer studies [5,153].…”

Section: Endgame: Diarrhoeamentioning

confidence: 99%

EnteropathogenicEscherichia coli: unravelling pathogenesis

2005

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Enteropathogenic Escherichia coli (EPEC) is a gram-negative bacterial pathogen that adheres to intestinal epithelial cells, causing diarrhoea. It constitutes a significant risk to human health and remains an important cause of infant mortality in developing countries. Although EPEC was the first E. coli strain to be implicated in human disease in the 1940s and 1950s, the mechanisms by which this pathogen induced diarrhoea remained a complete mystery throughout most of the 40 years since its description. It was only during the late 1980s that major advances were made in unravelling the mechanisms behind EPEC pathogenesis. Ever since, progress has been made at a stunning pace and there have been major breakthroughs in identifying the bacterial factors involved in attaching and effacing (A/E) lesion formation, host signal transduction pathways in response to EPEC infection and the genetic basis of EPEC pathogenesis. The rapid pace of discovery is a result of intensive research by investigators in this field and portends that EPEC will soon be among one of the most understood diarrhoea-causing infectious agents. This review aims to trace the progress of EPEC research since its existence was first reported by John Bray in 1945, highlighting the major findings that have revolutionised our understanding of EPEC pathogenesis.

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Section: Endgame: Diarrhoeamentioning

confidence: 99%

EnteropathogenicEscherichia coli: unravelling pathogenesis

2005

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“…In the United States, approximately 75 000 cases of O157:H7 infections are now estimated to occur annually (Mead et al 1999). Important virulence attributes that have been identified are shiga toxin production (Konowalchuk et al 1997), characteristic attaching and effacing (A ⁄ E) lesions and intimin protein production encoded by the eae gene (Knutton 1995;Whittam 1998). However, several lines of evidence underscore the fact that other unknown virulence factors may be expressed by E. coli O157:H7.…”

Section: Introductionmentioning

confidence: 99%

Design and evaluation of PCR primers which differentiateEscherichia coliO157:H7 and related serotypes

Rice

2009

Journal of Applied Microbiology

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Aims: To develop methods to differentiate Escherichia coli O157:H7 and related serotypes by the use of amplicon length polymorphism (ALP) analysis based on identifying DNA sequence deletions within highly homologous regions of three sequenced E. coli strains. Methods and Results: Potential primer locations along the ancestral genomic backbone were identified and evaluated against three sequenced genomes and then applied to a reference set of pathogenic E. coli strains. All 16 primer combinations generated the expected diagnostic fragments as predicted for the E. coli K12 MG1655, O157:H7 EDL933, and O157:H7B Sakai genomes. Conclusions: This study defines a collection of primers distributed along the length of the E. coli genome that were applied to ALP analysis methods to successfully differentiate between serotypes of E. coli O157:H7 and other E. coli serotypes. Significance and Impact of the Study: ALP‐PCR analysis method was validated as an independent method of classification when compared with that of rep‐PCR. The principles underlying ALP analysis can be readily applied for the detection and differentiation of other closely related microbial species because of the abundance of complete DNA sequence data for a large number of microbial genomes.

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“…Some important virulence attributes of E. coli O157 have been characterised, including VT production [4] and the characteristic attaching and e¡acing (A/E) lesion observed in the intestinal epithelium subsequent to colonisation by E. coli O157 [5]. Intimin, the protein product of the eae gene, was found to be critical for intimate contact during the production of the A/E lesion [6].…”

Section: Introductionmentioning

confidence: 99%

Use of representational difference analysis to identify Escherichia coli O157-specific DNA sequences

Allen

2001

FEMS Microbiology Letters

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Whereas several important virulence factors in Escherichia coli O157 have been identified, studies suggest they are not always essential and are probably insufficient to account for the severe clinical manifestation of E. coli O157 infection. Identification of putative virulence determinants is crucial to the understanding of bacterial pathogenesis and genomic comparison analysis may aid the characterisation of unidentified virulence attributes. In this study, representational difference analysis (RDA) was used for genomic comparison of E. coli O157 with the proposed ancestral strain, E. coli O55. Unique E. coli O157 gene sequences were isolated and one, termed RDA-1, taken forward for further analysis. Southern blotting with labelled RDA-1 as a probe showed it to be present in 77% of E. coli O157 isolates and absent in all non-E. coli O157 screened. Sequence flanking RDA-1 was obtained from a genomic clone identified by hybridisation, and contained an open reading frame predicted to encode a novel iron-regulated outer membrane protein. ß

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Cellular responses to enteropathogenicEscherichia coliinfection

Cited by 13 publications

References 43 publications

EnteropathogenicEscherichia coli: unravelling pathogenesis

EnteropathogenicEscherichia coli: unravelling pathogenesis

Design and evaluation of PCR primers which differentiateEscherichia coliO157:H7 and related serotypes

Use of representational difference analysis to identify Escherichia coli O157-specific DNA sequences

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