Cellular Responses to Combinations of Hyperthermia and Radiation

Dewey, William C.; Hopwood, Larry E.; Sapareto, Stephen A.; Gerweck, Leo E.

doi:10.1148/123.2.463

Cited by 840 publications

(303 citation statements)

References 23 publications

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“…29 This radiosensitization is probably due to the loss of cellular DNA polymerase activity by heat shock. 30 The synergistic interaction between two different modalities is maximal when cells are irradiated during heating.…”

Section: Discussionmentioning

confidence: 99%

Gene transfer into human prostate adenocarcinoma cells with an adenoviral vector: Hyperthermia enhances a double suicide gene expression, cytotoxicity and radiotoxicity

Lee¹,

Borrelli

2002

Cancer Gene Ther

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We have previously developed a recombinant adenovirus containing a fusion gene of Escherichia coli cytosine deaminase ( CD ) and herpes simplex virus type 1 thymidine kinase ( HSV -1 TK ) controlled by a cytomegalovirus ( CMV ) enhancer -promoter. This replication -incompetent adenovirus effectively transduced the CD -TK gene into human prostate adenocarcinoma DU -145 or PC -3 cells. Interestingly, heat shock at 418C for 4 hours elevated the level of CD -TK by approximately 5 -to 20 -fold at a multiplicity of infection ( MOI ) of 1. Heat -enhanced expression of CD -TK promoted cytotoxicity by 23 -, 9 -, or 47 -fold in the presence of 50 g / mL ganciclovir ( GCV ), 500 g / mL 5 -fluorocytosine ( 5 -FC ), or 50 g / mL GCV + 500 g / mL 5 -FC, respectively, at an MOI of 1. Moreover, there was an increase in radiosensitivity when adenovirus -infected cells were heated at 418C for 4 hours followed by irradiation in the presence of the prodrugs. Virus + heat + 1 g / mL GCV treatment increased radiosensitivity by a dose -modifying factor ( DMF ) of 2.2, whereas virus + heat + 10 g / mL 5 -FC exposure resulted in a DMF of 2.3. Radiosensitization was clearly enhanced as a result of combined prodrug exposure ( DMF = 4.4 ). Our results suggest that the efficiency in expression of suicide genes from an adenoviral vector used for cytotoxic anticancer therapy could be improved by combining heat treatment with radiation therapy.

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Section: Discussionmentioning

confidence: 99%

Gene transfer into human prostate adenocarcinoma cells with an adenoviral vector: Hyperthermia enhances a double suicide gene expression, cytotoxicity and radiotoxicity

Lee¹,

Borrelli

2002

Cancer Gene Ther

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“…This form was also used throughout this work, because previous calculations with coefficients taken from the literature (23) were in good agreement with experimental results (24,25). A slightly different form for the damage is based on the empirical observation that the required exposure time is exponentially dependent on the temperature, and that a critical temperature of 43°C was found to be the threshold for cell death in hyperthermia experiments (26). Thermal damage is then expressed in the equivalent heating time at 43°C t 43 ϭ ͐ K T( )Ϫ43°C d , where the empirical factor K has been determined to be approximately 2 for temperatures above 43°C and approximately 4 for temperatures below 43°C (27).…”

mentioning

confidence: 93%

Simulations of thermal tissue coagulation and their value for the planning and monitoring of laser‐induced interstitial thermotherapy (LITT)

Puccini

Bär

Bublat

et al. 2003

Magnetic Resonance in Med

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“…The response of tumours to hyperthermia is reported to be influenced by temperature distribution, pH, nutritional conditions, and perhaps also 02 concentration, i.e. factors that depend on tumour vascularization (Field & Bleehen, 1979;Dewey et al, 1980).…”

mentioning

confidence: 99%

Vascular structure of five human malignant melanomas grown in athymic nude mice

Solesvik¹,

Rofstad²,

Brustad³

1982

Br J Cancer

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Summary.-The vascular structure of 5 human malignant melanomas grown in athymic nude mice was characterized. The vessels were filled with a radio-opaque medium administered via the abdominal aorta of the mice. X-ray images, obtained from 720gum-thick tumour sections, provided qualitative information on the vascular structure of the tumours. Histograms for vessel length, surface, and volume as a function of vessel diameter were obtained by stereological analysis of 2,um-thick sections. The volume fraction of necrotic tissue in the tumours was also determined by stereological analysis. The 5 melanomas exhibited individual, characteristic vascular structures as well as individual, characteristic necrotic fractions. The total vessel length ranged from 32 + 2 to 80 + 4 mm, the total vessel surface from 1 6 + 0 1 to 3 8 + 0-2 mm2, and the total vessel volume from 0 009 + 0 001 to 0-022 + 0-002 mm3-all values per mm3 histologically intact tumour tissue. The necrotic fractions ranged from 30+1 to 49+4O %, and tended to be higher in the poorly than in the wellvascularized melanomas. The volume doubling times ranged from 4-2 to 216 days. Melanomas with short volume-doubling times had lower necrotic fractions and tended to be better vascularized than those with long volume-doubling times.

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Cellular Responses to Combinations of Hyperthermia and Radiation

Cited by 840 publications

References 23 publications

Gene transfer into human prostate adenocarcinoma cells with an adenoviral vector: Hyperthermia enhances a double suicide gene expression, cytotoxicity and radiotoxicity

Gene transfer into human prostate adenocarcinoma cells with an adenoviral vector: Hyperthermia enhances a double suicide gene expression, cytotoxicity and radiotoxicity

Simulations of thermal tissue coagulation and their value for the planning and monitoring of laser‐induced interstitial thermotherapy (LITT)

Vascular structure of five human malignant melanomas grown in athymic nude mice

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