Cellular responses of hyaluronic acid-coated chitosan nanoparticles

Almalik, Abdulaziz; Alradwan, Ibrahim; Majrashi, Majed A.; Alsaffar, Bashayer A.; Algarni, Abdulmalek T.; Alsuabeyl, Mohammed S.; AlRabiah, Haitham; Tirelli, Nicola; Alhasan, Ali H.

doi:10.1039/c8tx00041g

Cited by 23 publications

(25 citation statements)

References 40 publications

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“…The best transfecting particles were those with lower F A . Tirelli and co-workers deeply investigated the possibility to use a hyaluronan coating on chitosan-TPP nano-gels [81,86,87,88,89]. They reported that hyaluronic acid coating reduced the immunogenity and toxicity of nano-gels [87,88,89].…”

Section: Ionic Chitosan Micro- and Nano-gelsmentioning

confidence: 99%

“…Tirelli and co-workers deeply investigated the possibility to use a hyaluronan coating on chitosan-TPP nano-gels [81,86,87,88,89]. They reported that hyaluronic acid coating reduced the immunogenity and toxicity of nano-gels [87,88,89]. Almalik et al provided evidence that CD44-mediated endocytosis was responsible for the uptake of materials where HA was used as the coating agent [81,90].…”

Section: Ionic Chitosan Micro- and Nano-gelsmentioning

confidence: 99%

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Concepts for Developing Physical Gels of Chitosan and of Chitosan Derivatives

Sacco

Furlani

Marzo

et al. 2018

Gels

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Chitosan macro- and micro/nano-gels have gained increasing attention in recent years, especially in the biomedical field, given the well-documented low toxicity, degradability, and non-immunogenicity of this unique biopolymer. In this review we aim at recapitulating the recent gelling concepts for developing chitosan-based physical gels. Specifically, we describe how nowadays it is relatively simple to prepare networks endowed with different sizes and shapes simply by exploiting physical interactions, namely (i) hydrophobic effects and hydrogen bonds—mostly governed by chitosan chemical composition—and (ii) electrostatic interactions, mainly ensured by physical/chemical chitosan features, such as the degree of acetylation and molecular weight, and external parameters, such as pH and ionic strength. Particular emphasis is dedicated to potential applications of this set of materials, especially in tissue engineering and drug delivery sectors. Lastly, we report on chitosan derivatives and their ability to form gels. Additionally, we discuss the recent findings on a lactose-modified chitosan named Chitlac, which has proved to form attractive gels both at the macro- and at the nano-scale.

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Section: Ionic Chitosan Micro- and Nano-gelsmentioning

confidence: 99%

Section: Ionic Chitosan Micro- and Nano-gelsmentioning

confidence: 99%

Concepts for Developing Physical Gels of Chitosan and of Chitosan Derivatives

Sacco

Furlani

Marzo

et al. 2018

Gels

View full text Add to dashboard Cite

show abstract

“…Chitosan (CS) as a biocompatible, biodegradable, and nontoxic polymer has been under various investigations for the fabrication of the HA-coated NPs using the IG method. [18,19]. Thereafter, MT was encapsulated into the hydrophobic core of the nanoparticle containing a disulfide bond of Lipoic acid (LA) utilized as a reduction part [20].…”

Section: Introductionmentioning

confidence: 99%

Redox-Sensitive and Hyaluronic Acid-Functionalized Nanoparticles for Improving Breast Cancer Treatment by Cytoplasmic 17α-Methyltestosterone Delivery

et al. 2020

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Novel reduction-responsive hyaluronic acid–chitosan–lipoic acid nanoparticles (HACSLA-NPs) were designed and synthesized for effective treatment of breast cancer by targeting Cluster of Differentiation 44 (CD44)-overexpressing cells and reduction-triggered 17α-Methyltestosterone (MT) release for systemic delivery. The effectiveness of these nanoparticles was investigated by different assays, including release rate, 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT), lactate dehydrogenase (LDH), caspase-3 activity, Rhodamine 123 (RH-123), and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). In vitro experiments revealed that Methyltestosterone/Hyaluronic acid–chitosan–lipoic acid nanoparticles (MT/HACSLA-NPs) illustrated a sustained drug release in the absence of glutathione (GSH), while the presence of GSH led to fast MT release. HACSLA-NPs also showed high cellular internalization via CD44 receptors, quick drug release inside the cells, and amended cytotoxicity against positive CD44 BT-20 breast cancer cell line as opposed to negative CD44, Michigan Cancer Foundation-7 (MCF-7) cell line. These findings supported that these novel reduction-responsive NPs can be promising candidates for efficient targeted delivery of therapeutics in cancer therapy.

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“…Similarly, reactive oxygen species (ROS) production was suppressed when activated macrophages were treated with HA modified chitosan nanoparticles. The secretion of cytokines such as TNF-α and IL-1β were drastically reduced, indicating low immunogenicity of the HA-CS NPs without any collateral biological responses [51]. Zaki et al also demonstrated that HA-chitosan NPs were taken up at a relatively slower rate compared to NPs not coated with HA.…”

Section: Immunogenicity Of Ha-based Nanoparticlesmentioning

confidence: 99%

Hyaluronic Acid Derivatives for Targeted Cancer Therapy

Pramanik¹,

Jagirdar²

2021

Extracellular Matrix - Developments and Therapeutics

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Targeted therapeutics are considered next generation cancer therapy because they overcome many limitations of traditional chemotherapy. Cancerous cells may be targeted by various hyaluronic acid modified nanovehicles that kill these cells. Particularly, hyaluronic acid and its derivatives bind with high affinity to cell surface protein, CD44 enriched tumor cells. Moreover, these molecules have the added advantage of being biocompatible and biodegradable, and may be conjugated with a variety of drugs and drug carriers for developing various formulations as anti-cancer therapies such as nanogels, self-assembled and metallic nanoparticulates. In this chapter, we have covered various aspects of hyaluronic acid-modified delivery systems including strategies for synthesis, characterization, and biocompatibility. Next, the use of hyaluronic acid-modified systems as anti-cancer therapies is discussed. Finally, the delivery of small molecules, and other pharmaceutical agents are also elaborated in this chapter.

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Cellular responses of hyaluronic acid-coated chitosan nanoparticles

Abstract: In recent years, nanotechnology has been proven to offer promising biomedical applications for in vivo diagnostics and drug delivery, stressing the importance of thoroughly investigating the biocompatibility of potentially translatable nanoparticles (NPs).

Cited by 23 publications

References 40 publications

Concepts for Developing Physical Gels of Chitosan and of Chitosan Derivatives

Concepts for Developing Physical Gels of Chitosan and of Chitosan Derivatives

Redox-Sensitive and Hyaluronic Acid-Functionalized Nanoparticles for Improving Breast Cancer Treatment by Cytoplasmic 17α-Methyltestosterone Delivery

Hyaluronic Acid Derivatives for Targeted Cancer Therapy

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