Cellular Resistance in Listeriosis

Armstrong, A S; Sword, C. P.

doi:10.1093/infdis/114.3.258

Cited by 34 publications

(18 citation statements)

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“…A high fation of bactei level of parasitism seems to be required for initiaabnaess(13) lcohmpared tion of toxic manifestations. This is in accord ylococcs aureus after with the observation that derangements in the fluid and plasma clot cytoplasm of guinea pig macrophages by Listeria .e solid medium l)re-occurred only in cases where extensive intrasis and allowed loga-cellular multiplication had occurred (2). extracellular popula-…”

Section: Resultssupporting

confidence: 62%

“…Routine viability determinations indicated that continual phagocytosis of bacteria which proliferated freely in the medium were largely responsible for the cytotoxic manifestations. In their studies on the interaction of Listeria with guinea pig macrophages in culture, Armstrong and Sword (2) controlled extracellular bacterial growth by incorporating streptomycin into the tissue culture medium. Although the bacterial population increased during the 72-h experimental period, the investigators did not observe an overwhelming cytotoxic effect in stained cover slips.…”

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confidence: 99%

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Interaction of Virulent and Avirulent Listeria monocytogenes with Cultured Mouse Peritoneal Macrophages

Wilder

Edberg

1973

Infect Immun

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The interaction between smooth and rough Listeria monocytogenes and mouse peritoneal macrophages in culture was investigated. Initially, antibiotics were deleted from the culture medium, and no attempt other than the removal of unphagocytized bacteria by extensive washings was made to control extracellular growth. Under these conditions the monolayers were rapidly destroyed within an 8-h period, and this was associated with increases in the intracellular population of both strains. Extracellular viability counts revealed that washings failed to reduce the bacteria in the medium to less than 10 % of the original inoculum. Continuous phagocytosis of Li8teria which grew logarithmically in the maintenance media appears to account for the observed changes in the number of intracellular bacteria. The data also indicate that it is primarily the free bacteria in the culture medium which are responsible for the cytotoxic effects. In other experiments streptomycin-penicillin solutions were added to the maintenance media after an initial period of phagocytosis. In the presence of antibiotics, the total number of macrophages per field remained relatively constant, and no morphological alterations in the leukocyte cultures were observable. Extensive intracellular multiplication of either strain was not evident in fixed and stained cover slips. Viable intracellular counts reveal that after 24 h there is almost total killing of the rough variant, whereas the smooth strain tended towards complete survival.on August 8, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Resultssupporting

confidence: 62%

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confidence: 99%

Interaction of Virulent and Avirulent Listeria monocytogenes with Cultured Mouse Peritoneal Macrophages

Wilder

Edberg

1973

Infect Immun

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“…Animal infections with this group of organisms are characterized by a resistance against reinfection (Julianelle, 1941;Mackaness, 1962;Armstrong and Sword, 1964;Mackaness, 1964;Jenkin, Rowley, and Auzino, 1964;Rowley, Turner, and Jenkin, 1964). However, transfer of this resistance either to normal animals in vivo or into other macrophages in vitro via the serum has been unsuccessful (Miki and Mackaness, 1964;Mackaness, 1967).…”

Section: Discussionmentioning

confidence: 99%

Listeria monocytogenes meningitis: an opportunistic infection

Simpson

1971

Journal of Neurology, Neurosurgery & Psychiatry

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“…However, the present studies have demonstrated an absence of specificity in the antibacterial mechanisms developed during infection with S. iyphimurium. Infected animals inactivated Listeria monocytogenes in their spleens and livers and were completely resistant to challenge by 200 LDs0's of this organism, resistance to which depends upon enhancement of the microbicidal ability of host macrophages (7,8,23). Furthermore, Listeria-infected animals were resistant to S. typhimurium, and their macrophages possessed enhanced microbicidal activity for that organism in vitro; cross-resistance occurred in the absence of antibody capable of opsonizing or agglutinating S.…”

Section: Discussionmentioning

confidence: 99%

“…Acquired cellular resistance has been shown to occur in tuberculosis (4), brucellosis (5,6), and listeriosis (7,8). The increased microbicidal capacity of macrophages from animals infected with these bacteria is not specifically directed against the organisms which induced it (6), with the result that crossresistance has been found to exist between these infections (2, 6,9,10).…”

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confidence: 99%

Mechanisms of Acquired Resistance in Mouse Typhoid

Blanden

Mackaness

Collins

1966

The Journal of Experimental Medicine

270

129

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Experiments in vitro comparing normal mouse peritoneal macrophages with cells from Salmonella typhimurium-infected mice have shown that the "immune" macrophages have conspicuously enhanced microbicidal properties. Whereas normal macrophages could inactivate only 50 to 60% of intracellular S. typhimurium pretreated with immune serum, cells from infected animals killed virtually all ingested organisms and did so at an accelerated rate. Macrophages from Listeria monocytogenes-infected mice were shown to possess similarly enhanced microbicidal activity against S. typhimurium. Furthermore, the growth of S. typhimurium in the liver and spleen was more effectively restricted in Listeria-infected mice than in animals vaccinated with heat-killed S. typhimurium, even though the Listeria-infected animals possessed no demonstrable cross-reacting antibody to S. typhimurium. The lack of resistance in the mice vaccinated with heat-killed organisms could not be attributed to any deficiency of humoral factors, since the serum from these animals was as effective at promoting phagocytosis and killing by macrophages as serum from actively infected (and demonstrably resistant) mice. Conversely, Salmonella-infected mice were totally resistant to intravenous challenge with L. monocytogenes. The level of resistance in individual animals was related to the numbers of residual Salmonellae remaining in the tissues; mice with heavier residual infections being the more resistant. Specific antiserum from mice vaccinated with heat-killed S. typhimurium was found to be significantly protective only when the intraperitoneal route of challenge was employed. The foregoing studies have been interpreted to mean that enhancement of the microbicidal ability of macrophages is the mechanism of major importance in acquired resistance to S. typhimurium infection in mice.

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Cellular Resistance in Listeriosis

Cited by 34 publications

References 0 publications

Interaction of Virulent and Avirulent Listeria monocytogenes with Cultured Mouse Peritoneal Macrophages

Interaction of Virulent and Avirulent Listeria monocytogenes with Cultured Mouse Peritoneal Macrophages

Listeria monocytogenes meningitis: an opportunistic infection

Mechanisms of Acquired Resistance in Mouse Typhoid

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