Cellular repressor of E1A-stimulated genes is a bona fide lysosomal protein which undergoes proteolytic maturation during its biosynthesis

Increased Expression of Cellular Repressor of E1A-Stimulated Gene (CREG) in Gastric Cancer Patients: A Mechanism of Proliferation and Metastasis in Cancer

“…The relative percentage of migration of HUVEC co-cultured with MKN45 transfected with ShCREG4 was compared to that of negative control using t test. p \ 0.05 (*), p \ 0.01 (**) was considered a statistically significant difference Previous studies have shown that CREG is predominantly localized within intracellular compartments, and the intracellular CREG is localized in lysosome [13], but it is still not clear if CREG was expressed only in the cytoplasm or secreted into the medium, or both. To verify this question here, we detected the expression of CREG in GC cell strains and the culture medium, respectively.…”

“…Next, the study of the expression of CREG in GC cell strains would be desired. Although Schähsa et al [13] have reported that CREG proteins are predominantly localized within intracellular compartments, it is still not clear whether CREG is expressed only in the cytoplasm, outside the cell, or both. Therefore, this is the question that should be addressed first.…”

Cellular repressor of E1A-stimulated genes inhibits inflammation to decrease atherosclerosis in ApoE−/− mice

“…CREG is reported to predominantly localize within lysosomes [8][9][10]. Therefore, we investigated the effect of CREG on lysosome function in macrophages.…”

“…Recently, CREG is identified to be a bona fide lysosome protein and undergoes proteolytic maturation by lysosomal cysteine proteases in the course of their biosynthesis [8,9]. Expression of CREG is highest in lymph nodes and monocytes in both human and murine tissues as shown in EST databases on the NCBI-UniGene site.…”

CREG1 heterozygous mice are susceptible to high fat diet-induced obesity and insulin resistance

“…Subsequent studies showed that it is a small glycoprotein with 220 amino acid residues to be either secreted outside the cell or reside in intracellular membrane compartments [11, 12]. Human CREG1 has three glycosylation sites which are required for its binding to its putative receptor mannose 6 phosphate/insulin like growth factor 2 receptor (IGF2R) [13, 14].…”