Cellular refractive index comparison of various prostate cancer and noncancerous cell lines via photonic-crystal biosensor

DeLuna, Frank; Cadena, Melissa; Wang, Bingzhi; Sun, LuZhe; Ye, Jing Yong

doi:10.1117/12.2507505

Cited by 5 publications

(3 citation statements)

References 26 publications

(27 reference statements)

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“…Recent research is directed towards sensing the changes linked to tumor advancement within tissues and their microenvironment, aiming to facilitate the early-stage tumor detection [6][7][8][9][10][11] . Tumor progression induces stiffening of the tissue and increased disorder due to fibrosis, causing alterations in biomechanical properties, local refractive index, viscoelasticity, and surface roughness of cancerous tissues [12][13][14] . These changes will alter the scattering conditions in the tissues.…”

Section: Introductionmentioning

confidence: 99%

Tumour polyp detection using random lasing

Gayathri,

Suchand Sandeep,

Vijayan

et al. 2024

Biomedical Applications of Light Scattering XIV

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Tumors arise from the uncontrolled growth of cells and can be benign or cancerous. The size of the tumor is one of the key factors in assessing its malignancy potential. Generally, larger tumor polyp tends to have higher risk of developing into a cancer. Smaller polyps often start benign but some of it evolve over time to adenomatous or cancerous and extend to other parts of the body. To mitigate this risk, even smaller polyps are usually removed if found during screening. However, the detection of small polyps, especially flat or sessile types, remains a challenge. Advanced techniques are being developed to identify early-stage tumors by studying biomechanical, biochemical, and morphological changes. Tumor progression alters the viscoelasticity, local refractive index, and surface roughness, increasing tissue disorder both structurally and optically. This disorder can localize light through multiple scattering and can be utilized to provide cavity feedback for lasing emission called 'random lasing'. In this work, we simulate a tumor polyp growth in a phantom tissue impregnated with a gain medium and investigate the resulting random lasing emission. We find that the emission properties such as the intensity, lasing threshold, emission wavelength, and linewidth are all influenced by the presence of the polyp and this technique could precisely identify even polyps of size ~ millimeters. Overall, this research showcases the potential of random lasing to investigate disorder-induced changes for early and sensitive detection of tumor and identification of tissue abnormalities.

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Section: Introductionmentioning

confidence: 99%

Tumour polyp detection using random lasing

Gayathri,

Suchand Sandeep,

Vijayan

et al. 2024

Biomedical Applications of Light Scattering XIV

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“…Hence, several techniques are being developed to study the morphological, biochemical, and biomechanical changes in tumor regions and their microenvironments [21][22][23][24][25]. It has been found that tumor progression causes changes in the local refractive index, biomechanical properties, surface roughness, and viscoelasticity of the tissues [26][27][28]. Changes in these properties will modify the scattering scenario and thus can be detected using random lasing.…”

Section: Introductionmentioning

confidence: 99%

Random Lasing for Bimodal Imaging and Detection of Tumor

Gayathri,

Suchand Sandeep,

Vijayan

et al. 2023

Biosensors

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The interaction of light with biological tissues is an intriguing area of research that has led to the development of numerous techniques and technologies. The randomness inherent in biological tissues can trap light through multiple scattering events and provide optical feedback to generate random lasing emission. The emerging random lasing signals carry sensitive information about the scattering dynamics of the medium, which can help in identifying abnormalities in tissues, while simultaneously functioning as an illumination source for imaging. The early detection and imaging of tumor regions are crucial for the successful treatment of cancer, which is one of the major causes of mortality worldwide. In this paper, a bimodal spectroscopic and imaging system, capable of identifying and imaging tumor polyps as small as 1 mm2, is proposed and illustrated using a phantom sample for the early diagnosis of tumor growth. The far-field imaging capabilities of the developed system can enable non-contact in vivo inspections. The integration of random lasing principles with sensing and imaging modalities has the potential to provide an efficient, minimally invasive, and cost-effective means of early detection and treatment of various diseases, including cancer.

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“…As the protein expression changes when the cells undergo EMT, we believe these biochemical changes can be correlated to changes in refractive index of the cell membrane. We have previously demonstrated the ability to use cellular membrane RI, as a novel contrast parameter to allow for label-free detection of prostate cancer cells [9][10][11] In this paper, we report a novel method to characterize prostate cancer cells undergoing EMT based on the cell's membrane RI via the use of a label-free biosensing system constructed with a Photonic Crystal Biosensor in a TotalInternal Reflection (PC-TIR) configuration. EMT in prostate cells lines was induced by treating the cells with Transforming Growth Factor Beta 1 (TGF-β1).…”

Section: Introductionmentioning

confidence: 99%

Epithelial-mesenchymal transition of prostate cancer cells monitored with a photonic crystal biosensor

Cadena

DeLuna

Baryeh

et al. 2020

Label-Free Biomedical Imaging and Sensing (LBIS) 2020

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During prostate cancer progression, cancerous epithelial cells can undergo epithelial-mesenchymal transition (EMT). EMT is a crucial mechanism for the invasion and metastasis of epithelial tumors characterized by the loss of cell-cell adhesion and increased cell mobility. It is associated with biochemical changes such as epithelial cell markers E-cadherin and occludins being downregulated, and mesenchymal markers vimentin and N-cadherin being upregulated. These changes in protein expression, specifically in the cell membrane, may be monitored via biophysical principles, such as changes in the refractive index (RI) of the cell membrane. In our previous research, we demonstrated the feasibility of using cellular RI as a unique contrast parameter to accomplish label-free detection of prostate cancer cells. In this paper, we report the use of our Photonic-Crystal biosensor in a Total-Internal-Reflection (PC-TIR) configuration to construct a label-free biosensing system, which allows for ultra-sensitive quantification of the changes in cellular RI due to EMT. We induced prostate cancer cells to undergo EMT by exposing these cells to soluble Transforming Growth Factor Beta 1 (TGF-β1). The biophysical characteristics of the cellular RI were quantified extensively in comparison to non-induced cancer cells. Our study shows promising clinical potential in utilizing the PC-TIR biosensing system not only to detect prostate cancer cells, but also to evaluate changes in prostate cancer cells due to EMT.

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Cellular refractive index comparison of various prostate cancer and noncancerous cell lines via photonic-crystal biosensor

Cited by 5 publications

References 26 publications

Tumour polyp detection using random lasing

Tumour polyp detection using random lasing

Random Lasing for Bimodal Imaging and Detection of Tumor

Epithelial-mesenchymal transition of prostate cancer cells monitored with a photonic crystal biosensor

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