Cellular Populations Isolated from Newborn Mouse Skin Including Mesenchymal Stem Cells

Kimlin, Lauren; Virador, Victoria

doi:10.1007/978-1-62703-330-5_17

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…Similar to NSCs, isolation of CSCs from tumor tissues, including thyroid cancers, have been performed by different approaches, including antigen-directed FACS sorting, side population and Aldefluor assay [ 52 , 68 ]. Although in vitro culture and maintenance of CSCs is difficult, the continuous development of new technologies has allowed the CSC culture as cell floating spheroids in non-adherent plates or 3D culture in reconstituted basal membranes (Matrigel) [ 69 , 70 ]. An important feature of CSCs, at various limiting dilution, is the ability to reproduce the original tumors in vivo.…”

Section: Normal and Cscs: Characteristics And Classificationmentioning

confidence: 99%

The Possible Role of Cancer Stem Cells in the Resistance to Kinase Inhibitors of Advanced Thyroid Cancer

Gianì

Vella

Tumino

et al. 2020

Cancers

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Target therapy with various kinase inhibitors (KIs) has been extended to patients with advanced thyroid cancer, but only a subset of these compounds has displayed efficacy in clinical use. However, after an initial response to KIs, dramatic disease progression occurs in most cases. With the discovery of cancer stem cells (CSCs), it is possible to postulate that thyroid cancer resistance to KI therapies, both intrinsic and acquired, may be sustained by this cell subtype. Indeed, CSCs have been considered as the main drivers of metastatic activity and therapeutic resistance, because of their ability to generate heterogeneous secondary cell populations and survive treatment by remaining in a quiescent state. Hence, despite the impressive progress in understanding of the molecular basis of thyroid tumorigenesis, drug resistance is still the major challenge in advanced thyroid cancer management. In this view, definition of the role of CSCs in thyroid cancer resistance may be crucial to identifying new therapeutic targets and preventing resistance to anti-cancer treatments and tumor relapse. The aim of this review is to elucidate the possible role of CSCs in the development of resistance of advanced thyroid cancer to current anti-cancer therapies and their potential implications in the management of these patients.

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Section: Normal and Cscs: Characteristics And Classificationmentioning

confidence: 99%

The Possible Role of Cancer Stem Cells in the Resistance to Kinase Inhibitors of Advanced Thyroid Cancer

Gianì

Vella

Tumino

et al. 2020

Cancers

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“…Existing evidence suggests that skin might contain an MSC-like cell population ( Kimlin and Virador, 2013 ; Toma et al, 2001 ; Vaculik et al, 2012 ; Viswanathan et al, 2019 ). However, their biological properties and contribution to hematopoiesis and leukemia remain unexplored.…”

Section: Introductionmentioning

confidence: 99%

Skin mesenchymal niches maintain and protect AML-initiating stem cells

Sandhow

Cai

Leonard

et al. 2023

Journal of Experimental Medicine

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Leukemia cutis or leukemic cell infiltration in skin is one of the common extramedullary manifestations of acute myeloid leukemia (AML) and signifies a poorer prognosis. However, its pathogenesis and maintenance remain understudied. Here, we report massive AML cell infiltration in the skin in a transplantation-induced MLL-AF9 AML mouse model. These AML cells could regenerate AML after transplantation. Prospective niche characterization revealed that skin harbored mesenchymal progenitor cells (MPCs) with a similar phenotype as BM mesenchymal stem cells. These skin MPCs protected AML-initiating stem cells (LSCs) from chemotherapy in vitro partially via mitochondrial transfer. Furthermore, Lama4 deletion in skin MPCs promoted AML LSC proliferation and chemoresistance. Importantly, more chemoresistant AML LSCs appeared to be retained in Lama4−/− mouse skin after cytarabine treatment. Our study reveals the characteristics and previously unrecognized roles of skin mesenchymal niches in maintaining and protecting AML LSCs during chemotherapy, meriting future exploration of their impact on AML relapse.

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“…Existing evidence suggests that skin might contain an MSC-like cell population. [16][17][18][19] However, their biological properties and contribution to hematopoiesis and leukemia remain unexplored. Knowledge of this would be essential for understanding the pathology of extramedullary leukemia in the skin, and would thus provide new insights into identifying new potential therapeutic targets that may be translated into new treatment strategies for AML patients with leukemia cutis.…”

Section: Introductionmentioning

confidence: 99%

Skin mesenchymal niches maintain and protect AML-initiating stem cells

Sandhow

Cai

Xiao

et al. 2022

Preprint

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Leukemia cutis or leukemic cell infiltration in skin is one of the common extramedullary manifestations of acute myeloid leukemia (AML) and signifies a poorer prognosis. However, its pathogenesis and maintenance remain understudied. Here, we report massive AML cell infiltration in mouse skin. These cells were retained in the skin post-chemotherapy and could regenerate AML post-transplantation. The niche characterization revealed that skin harbored mesenchymal progenitor cells (MPCs) with a similar phenotype as BM mesenchymal stem cells. These skin MPCs maintained and protected AML-initiating stem cells (LSCs) from chemotherapy in vitro possibly via mitochondrial transfer. Furthermore, Lama4 deletion in skin MPCs promoted AML LSC proliferation and chemoresistance. Importantly, more chemoresistant AML LSCs were retained in Lama4−/- mouse skin post-cytarabine treatment. Our study reveals the characteristics and previously unrecognized roles of skin mesenchymal niches in maintaining AML LSCs and protecting them during chemotherapy, meriting future exploration of their impact on AML relapse.Key pointsLeukemic cells infiltrated in skin are capable to generate AML post-transplantationMesenchymal progenitors in skin contribute to forming the niche for AML cellsSkin mesenchymal progenitors protect AML stem cells possibly by mitochondrial transferLama4 deletion in skin mesenchymal niche promotes AML cell proliferation and chemoresistance to cytarabine

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Cellular Populations Isolated from Newborn Mouse Skin Including Mesenchymal Stem Cells

Cited by 4 publications

References 9 publications

The Possible Role of Cancer Stem Cells in the Resistance to Kinase Inhibitors of Advanced Thyroid Cancer

The Possible Role of Cancer Stem Cells in the Resistance to Kinase Inhibitors of Advanced Thyroid Cancer

Skin mesenchymal niches maintain and protect AML-initiating stem cells

Skin mesenchymal niches maintain and protect AML-initiating stem cells

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