Cellular Permeability and Extracellular Formation of Glutamic Acid in Brevibacterium flavum

Shiio, Isamu; Ôtsuka, Shin-Ichirô; Katsuya, Noboru

doi:10.1093/oxfordjournals.jbchem.a127706

Cited by 44 publications

(15 citation statements)

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“…Moreover, the increase of cellular permeability to glutamate has also been found in the cells made capable of producing glutamate in the biotin-rich medium by the addition of penicillin or Tween 60 to the culture (4). A similar relationship between the ability to produce glutamate and cellular permeability to glutamate was also found in the case of the other strain of glutamate-producing bacteria, Micrococcus glutamicus (ATCC No.…”

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confidence: 57%

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Fatty Acid Composition of Cell Wall-Cell Membrane Fraction From Brevibacterium Flavum

Ôtsuka

Shiio

1968

J. Gen. Appl. Microbiol.

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confidence: 57%

“…Furthermore, they have suggested, considering the wellknown roles of biotin in the biosynthesis of fatty acids, that the increase of permeability to glutamate caused by the deficiency of biotin would be the result of biochemical changes of lipids, probably of lipoprotein, in the cell membrane (1)(2)(3)(4)(5).…”

mentioning

confidence: 99%

Fatty Acid Composition of Cell Wall-Cell Membrane Fraction From Brevibacterium Flavum

Ôtsuka

Shiio

1968

J. Gen. Appl. Microbiol.

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“…There have been reports (Shiio et al, 1963;Otsuka et al, 1964;Shiio and Uchio, 1969) on the effect of penicillin and its addition time on amino acid production, and these were investigated in the present study. Varying levels of penicillin were added to the culture medium at 0, 16, 18, 20h, 22 and 24 h. Optimum cultural conditions were maintained.…”

Section: Influence Of Penicillin and Addition Timementioning

confidence: 96%

Studies on lysine production by Bacillus megaterium

Ekwealor¹,

Obeta²

2005

Afr. J. Biotechnol.

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A Lysine-producing strain recovered from soil was found to produce large amount of the amino acid. The bacterium identified as Bacillus megaterium SP 14 accumulated a lysine yield of 3.56 mg/ml in a broth culture in 96 h. Fermentation experiments show that 8.0% (w/v) glucose and 4.0% (w/v) ammonium chloride used as sources of carbon and nitrogen, respectively, in a medium/fermenter volume ratio of 25.0%, influenced accumulation of the amino acid. Amino acids other than the aspartate family at 0.01% (w/v) stimulated growth and improved lysine yield. Addition of 0.01 unit/ml penicillin to the fermentation medium, immediately after inoculation, stimulated growth and appreciably enhanced lysine accumulation.

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“…The generally accepted hypothesis for glutamate secretion under biotin limitation V"leak model") is based on the observation that lack of biotin decreases fatty acid synthesis, causing alterations in the plasma membrane, i.e., decreasing the lipid content and changing the phospholipid and fatty acid composition (45). Thus, the plasma membrane is postulated to become permeable to glutamate, leading to secretion -by passive diffusion (4,9,21,22,30,(39)(40)(41). The internal concentration of glutamate then decreases to such an extent that the feedback inhibition of glutamate synthesis is abolished.…”

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Membrane alteration is necessary but not sufficient for effective glutamate secretion in Corynebacterium glutamicum

1990

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We showed recently that secretion of glutamate in biotin-limited cells of Corynebacterium glutamicum is mediated by carrier systems in the plasma membrane (C. Hoischen -and R. Kramer, Arch. Microbiol. 151:342-347, 1989). In view of the generally accepted hypothesis that glutamate efflux is directly caused by alterations of the membrane, it was necessary to examine the kind of correlation between changes in lipid content and composition of the bacterial membrane and glutamate secretion activity. Two new experimental approaches were'used. (i) Changes in lipid content and composition were analyzed in glutamate-producing cells which were forced to switch to nonproducers by addition of biotin in a short-term fermentation. (ii) The time courses of both the fatty acid or phospholipid composition and the efflux activity were analyzed within the first minutes of the switch from high to low secretion activity. The following results were obtained. (i) The time course of the change i,n fatty acid or phospholipid content and composition was not related to the change in secretion behavior. (ii) There was no specific fatty acid or phospholipid compound which regulated glutamate efflux. (iii) High efflux activity could only be induced when the total lipid content of the membrane was reduced.

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Cellular Permeability and Extracellular Formation of Glutamic Acid in Brevibacterium flavum

Cited by 44 publications

References 0 publications

Fatty Acid Composition of Cell Wall-Cell Membrane Fraction From Brevibacterium Flavum

Fatty Acid Composition of Cell Wall-Cell Membrane Fraction From Brevibacterium Flavum

Studies on lysine production by Bacillus megaterium

Membrane alteration is necessary but not sufficient for effective glutamate secretion in Corynebacterium glutamicum

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