Cellular O‐Glycome Reporter/Amplification (CORA): Analytical and Preparative Tools to Study Mucin‐Type O‐Glycans of Living Cells

Kudelka, Matthew R; Li, Zhonghua; Chernova, Tatiana A.; Smith, David F.; Song, Xuezheng; Cummings, Richard D.; Ju, Tongzhong

doi:10.1002/cpz1.142

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…Molecular-level evidence of the buildup of glycans on thioglycosides 4 – 6 within cells would confirm this proposed mechanism. Cummings, Neelamagham, Wang, and others have developed and applied mass spectrometry-based approaches to detect elaborated glycans on decoy scaffolds, facilitating the structural characterization of mammalian glycans. ,− These approaches could be translated to the study of elaborated glycans on decoy scaffolds in bacteria. If this proposed mechanism is correct, novel thioglycosides could analogously serve as readouts of bacterial glycan biosynthesis, ultimately enabling glycomic analyses that yield structural information.…”

Section: Discussionmentioning

confidence: 99%

Thioglycosides Act as Metabolic Inhibitors of Bacterial Glycan Biosynthesis

Quintana,

Paul,

Chowdhury

et al. 2023

ACS Infect. Dis.

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Glycans that coat the surface of bacteria are compelling antibiotic targets because they contain distinct monosaccharides that are linked to pathogenesis and are absent in human cells. Disrupting glycan biosynthesis presents a path to inhibiting the ability of a bacterium to infect the host. We previously demonstrated that O-glycosides act as metabolic inhibitors and disrupt bacterial glycan biosynthesis. Inspired by a recent study which showed that thioglycosides (S-glycosides) are 10 times more effective than O-glycosides at inhibiting glycan biosynthesis in mammalian cells, we crafted a panel of Sglycosides based on rare bacterial monosaccharides. The novel thioglycosides altered glycan biosynthesis and fitness in pathogenic bacteria but had no notable effect on glycosylation or growth in beneficial bacteria or mammalian cells. In contrast to findings in mammalian cells, S-glycosides and O-glycosides exhibited comparable potency in bacteria. However, S-glycosides exhibited enhanced selectivity relative to O-glycosides. These novel metabolic inhibitors will allow selective perturbation of the bacterial glycocalyx for functional studies and set the stage to expand our antibiotic arsenal.

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Section: Discussionmentioning

confidence: 99%

Thioglycosides Act as Metabolic Inhibitors of Bacterial Glycan Biosynthesis

Quintana,

Paul,

Chowdhury

et al. 2023

ACS Infect. Dis.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Cellular O‐Glycome Reporter/Amplification (CORA): Analytical and Preparative Tools to Study Mucin‐Type O‐Glycans of Living Cells

Cited by 2 publications

References 41 publications

Thioglycosides Act as Metabolic Inhibitors of Bacterial Glycan Biosynthesis

Thioglycosides Act as Metabolic Inhibitors of Bacterial Glycan Biosynthesis

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

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