Cellular nanotechnologies: Orchestrating cellular processes by engineering silicon nanowires architectures

Yoh, HaoZhe; Aslanoglou, Stella; Lestrell, Esther; Shokouhi, Ali-Reza; Belcher, Simon; Thissen, Helmut; Voelcker, Nicolas H.; Elnathan, Roey

doi:10.1016/b978-0-12-821351-3.00013-6

Cited by 7 publications

(5 citation statements)

References 124 publications

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“…Interestingly, similar cellular effects are also observed in cells on nanoscale topographical features in which vertically aligned nanopillars or nanowires also stimulate nuclear deformation. In this scenario, the observed changes to gene expression arising from a nanotopographical influence have been attributed to an increase in proximity between the chromosomes and nuclear membrane . More recently, Hanson et al showed that the curvature of nuclear deformation can be controlled by varying the geometry of the nanopillar arrays as determined by nuclear stiffness, while further confirming the role of actomyosin tension in the establishment of these nuclear deformations.…”

Section: Cellular Mechanisms Of Micro- and Nanotopographic Mechanosen...mentioning

confidence: 96%

“…In this scenario, the observed changes to gene expression arising from a nanotopographical influence have been attributed to an increase in proximity between the chromosomes and nuclear membrane. 193 More recently, Hanson et al showed that the curvature of nuclear deformation can be controlled by varying the geometry of the nanopillar arrays as determined by nuclear stiffness, while further confirming the role of actomyosin tension in the establishment of these nuclear deformations. Interestingly, they were also able to shed light on novel roles of the intermediate filament cytoskeletal networking in this process, exemplifying the importance of this network in resisting actin-derived tension.…”

Section: Cellular Mechanisms Of Micro-and Nanotopographic Mechanosensingmentioning

confidence: 99%

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The Bumpy Road to Stem Cell Therapies: Rational Design of Surface Topographies to Dictate Stem Cell Mechanotransduction and Fate

Carthew

Taylor

Garcia-Cruz

et al. 2022

ACS Appl. Mater. Interfaces

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Cells sense and respond to a variety of physical cues from their surrounding microenvironment, and these are interpreted through mechanotransductive processes to inform their behavior. These mechanisms have particular relevance to stem cells, where control of stem cell proliferation, potency, and differentiation is key to their successful application in regenerative medicine. It is increasingly recognized that surface micro- and nanotopographies influence stem cell behavior and may represent a powerful tool with which to direct the morphology and fate of stem cells. Current progress toward this goal has been driven by combined advances in fabrication technologies and cell biology. Here, the capacity to generate precisely defined micro- and nanoscale topographies has facilitated the studies that provide knowledge of the mechanotransducive processes that govern the cellular response as well as knowledge of the specific features that can drive cells toward a defined differentiation outcome. However, the path forward is not fully defined, and the “bumpy road” that lays ahead must be crossed before the full potential of these approaches can be fully exploited. This review focuses on the challenges and opportunities in applying micro- and nanotopographies to dictate stem cell fate for regenerative medicine. Here, key techniques used to produce topographic features are reviewed, such as photolithography, block copolymer lithography, electron beam lithography, nanoimprint lithography, soft lithography, scanning probe lithography, colloidal lithography, electrospinning, and surface roughening, alongside their advantages and disadvantages. The biological impacts of surface topographies are then discussed, including the current understanding of the mechanotransductive mechanisms by which these cues are interpreted by the cells, as well as the specific effects of surface topographies on cell differentiation and fate. Finally, considerations in translating these technologies and their future prospects are evaluated.

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Section: Cellular Mechanisms Of Micro- and Nanotopographic Mechanosen...mentioning

confidence: 96%

Section: Cellular Mechanisms Of Micro-and Nanotopographic Mechanosensingmentioning

confidence: 99%

The Bumpy Road to Stem Cell Therapies: Rational Design of Surface Topographies to Dictate Stem Cell Mechanotransduction and Fate

Carthew

Taylor

Garcia-Cruz

et al. 2022

ACS Appl. Mater. Interfaces

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“…[ 164 ] Necessary to achieve such a translational goal, it will be crucial to develop efficient, rapid, nondestructive, and scalable routes for direct access to intracellular environments. [ 165 ] Such advances would unlock the potential for breakthroughs in fundamental cellular studies and enable the orchestration of cellular reprogramming with higher efficiency and greater predictability. The most applicable nanomaterials that have been used for cell reprogramming can be categorized into three groups called organic, inorganic, and hybrid nanocarriers ( Figure ).…”

Section: Cell Reprogramming By Nonviral Vectorsmentioning

confidence: 99%

Changing Fate: Reprogramming Cells via Engineered Nanoscale Delivery Materials

Dehnavi¹,

Zadeh

Harvey

et al. 2022

Advanced Materials

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The incorporation of nanotechnology in regenerative medicine is at the nexus of fundamental innovations and early‐stage breakthroughs, enabling exciting biomedical advances. One of the most exciting recent developments is the use of nanoscale constructs to influence the fate of cells, which are the basic building blocks of healthy function. Appropriate cell types can be effectively manipulated by direct cell reprogramming; a robust technique to manipulate cellular function and fate, underpinning burgeoning advances in drug delivery systems, regenerative medicine, and disease remodeling. Individual transcription factors, or combinations thereof, can be introduced into cells using both viral and nonviral delivery systems. Existing approaches have inherent limitations. Viral‐based tools include issues of viral integration into the genome of the cells, the propensity for uncontrollable silencing, reduced copy potential and cell specificity, and neutralization via the immune response. Current nonviral cell reprogramming tools generally suffer from inferior expression efficiency. Nanomaterials are increasingly being explored to address these challenges and improve the efficacy of both viral and nonviral delivery because of their unique properties such as small size and high surface area. This review presents the state‐of‐the‐art research in cell reprogramming, focused on recent breakthroughs in the deployment of nanomaterials as cell reprogramming delivery tools.

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“…The advancement of nanofabrication alongside with nanobiotechnology in recent years has enhanced the engineered nanobio cellular interfaces and widened their applications. [1][2][3][4][5][6] In particular, vertically aligned nanoneedles (NNs) of semiconducting, inorganic, and polymeric materials-such as nanowires, [7][8][9][10] nanostraws, 11,12 nanotubes (NTs), [13][14][15][16] and their electroactive analogues, [17][18][19] -have been shown to be promising tools for complex cellular manipulation, such as mechanotransduction, [20][21][22][23][24][25] biosensing, 26,27 immunomodulation, 28 in vivo and ex vivo gene editing, [29][30][31][32] intracellular delivery, [33][34][35][36][37][38][39] and even cellular reprogramming. [40][41][42] Fabrication complexity [43][44][45][46]…”

Section: Introductionmentioning

confidence: 99%

The influence of dysfunctional actin on polystyrene-nanotube-mediated mRNA nanoinjection into mammalian cells

et al. 2023

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Cellular nanotechnologies: Orchestrating cellular processes by engineering silicon nanowires architectures

Cited by 7 publications

References 124 publications

The Bumpy Road to Stem Cell Therapies: Rational Design of Surface Topographies to Dictate Stem Cell Mechanotransduction and Fate

The Bumpy Road to Stem Cell Therapies: Rational Design of Surface Topographies to Dictate Stem Cell Mechanotransduction and Fate

Changing Fate: Reprogramming Cells via Engineered Nanoscale Delivery Materials

The influence of dysfunctional actin on polystyrene-nanotube-mediated mRNA nanoinjection into mammalian cells

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