Cellular metabolomics reveals glutamate and pyrimidine metabolism pathway alterations induced by BDE-47 in human neuroblastoma SK-N-SH cells

Zhi, Tian; Li, Yunxiu; Jiang, Yousheng; Cheng, Jinquan; Xu, Shunqing

doi:10.1016/j.ecoenv.2019.109427

Cited by 22 publications

(13 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, our research on metabolic pathways found that changes in nucleotides caused disturbances in the purine metabolic pathway. Studies have shown that abnormal purine metabolism has a destructive effect on the central nervous system 37,38 , which is consistent with the results caused by sphingolipid metabolism.…”

Section: Discussionsupporting

confidence: 86%

Metabolomics-based study reveals the effect of lead (Pb) in the culture environment on Whitmania pigra

Luo

Meng

Chen

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Whitmania pigra, called Mahuang (MH) in Chinese, has been used as a traditional Chinese medicine for many years and is susceptible to Pb exposure in aquaculture environments. To understand the impact of Pb in the culture environment on MHs, we carried out a 50-day culture of MHs in environments with different levels of Pb pollution. Then, tissue samples of MHs reared in the different Pb-polluted environments were collected and analysed by UPLC-Q/TOF-MS. The results showed that the Pb residue in MHs increased with increasing Pb in the culture environment. There was no significant difference in MH Pb content (P < 0.05) between the low-Pb residue group (PbL) and the blank control group (BC), and those of the middle-Pb residue group (PbM) and the high-Pb residue group (PbH) were significantly different from that of the BC group. Metabolomics results showed significant changes in 24 metabolites in the PbL, PbM and PbH groups, some of which were dose-dependent. These metabolites were mainly lipids, nucleotides, and dipeptides, which are involved in metabolic pathways such as glycerophospholipid metabolism, sphingolipid metabolism, and nucleotide metabolism. Overall, the results proved that metabolomics can be an effective tool to understand the effects of Pb on the metabolic responses of MHs.

show abstract

Section: Discussionsupporting

confidence: 86%

Metabolomics-based study reveals the effect of lead (Pb) in the culture environment on Whitmania pigra

Luo

Meng

Chen

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The depletion of tetrahydrofolate causes cell death by suppressing DNA and RNA production (Kanarek et al, 2018). Toxicants, such as deoxynivalenol, could cause amino acids and nucleotides deficiency through repressing a variety of biosynthesis of amino acids and nucleotides, finally lead to hepatotoxicity, immunotoxicity and other toxicity (Ji et al, 2016;Geng et al, 2019;Tang et al, 2019). Furthermore, disruption of the TCA cycle and glutamate metabolism plays an essential role in the pathogenesis of amyotrophic lateral sclerosis (ALS), a disease that is closely related to the death of the neurocytes (Veyrat-Durebex et al, 2016).…”

Section: The Relationship Between Amino Acid Metabolism and Toxicitymentioning

confidence: 99%

Kunxian Capsule for Rheumatoid Arthritis: Inhibition of Inflammatory Network and Reducing Adverse Reactions Through Drug Matching

Tang

Zeng

et al. 2020

Front. Pharmacol.

View full text Add to dashboard Cite

Tripterygium wilfordii Hook.f and Tripterygium hypoglaucum (H.Lév.) Hutch is effective herbs to prevent aggravation of Rheumatoid arthritis (RA). However, both of them show severe side effects in the reproductive system and other systems. Kunxian Capsule (KX), a Traditional Chinese Medicine (TCM) patent prescription, comprised of 4 herbs, including H.Lév. Hutch, is reported to be an available prescription in treating RA with fewer side effects as compares to Tripterygium tablets. To reveal the pharmacological mechanism of KX in RA treatment and side effect alleviation, we collected related information of KX from open-access databases and performed various analyses. 1354 targets were identified in KX. These targets were enriched in the calcium signaling pathway, cAMP signaling pathway, cGMP-PKG signaling pathway and PI3K-AKT signaling pathway, forming biological functions, such as cofactor binding, coenzyme binding, etc. These pathways or functions mostly affect cell cycle, differentiation, and maturation of Th17 cells, macrophage, and synovial fibroblast. These targets also act on the IL-17 signaling pathway, Th17 cell differentiation signaling pathway and TNF signaling pathway, which is related to inflammation response inhibition. Next, a disease network was constructed, which indicated IMPDH2, MTHFD1 are the key genes answering for the side effects of H.Lév. Hutch. The side effect-related genes lead to the negative regulation of nucleic acid, which could be restored by the rest 3 herbs through some positive amino acid metabolism. In conclusion, KX is a relatively safe alternative approach in RA intervention.

show abstract

“…In particular, cell apoptosis is bio‐monitored by p53 activation, which is an effective indicator that a cell is apoptotic and results from cytochrome c release from the mitochondrion into the cytosol 43 . Apoptotic stimulation leads to changes in Bcl2 and Bax mRNA expression levels with subsequent alterations in Bcl2 / Bax , which eventually affect cytochrome c release 46 . In addition, the significant role of the anti‐apoptotic genes, including the expression of ratio of Bcl2 and Bax , has made it evident that decreased Bcl2 / Bax is correlated in cell death 43,47 .…”

Section: Discussionmentioning

confidence: 99%

“…43 Apoptotic stimulation leads to changes in Bcl2 and Bax mRNA expression levels with subsequent alterations in Bcl2/Bax, which eventually affect cytochrome c release. 46 In addition, the significant role of the anti-apoptotic genes, including the expression of ratio of Bcl2 and Bax, has made it evident that decreased Bcl2/Bax is correlated in cell death. 43,47 The activation of caspases results from the oligomerization of apoptotic protease activating factor-1, which subsequently initiates the activation of Caspase3, a downstream caspase, which is considered an important regulator of apoptosis.…”

Section: Apoptosis-related Gene Expressionmentioning

confidence: 99%

Hepatic oxidative stress, DNA damage and apoptosis in adult zebrafish following sub‐chronic exposure to BDE‐47 and BDE‐153

Meng

Chen

Gyimah

et al. 2020

Environmental Toxicology

View full text Add to dashboard Cite

Polybrominated diphenyl ethers (PBDEs) are ubiquitous and prolific contaminant in both the abiotic and biotic environment because of the wide industrial applications of these chemicals. In the present study, the effects of 2,2 0 ,4,4 0-tetrabrominateddiphenyl ether (BDE-47) and 2,2 0 ,4,4 0 ,5,5 0-hexabromodiphenyl ether (BDE-153) exposure on the induction of hepatic oxidative stress, DNA damage, and the expression of apoptosis-related genes in adult zebrafish were investigated. The activities of antioxidant enzymes, such as catalase and superoxide dimutase, significantly increased when adult zebrafish was exposed to various concentrations of BDE-47 and BDE-153 for 7 and 15 days. BDE-47 and BDE-153 elicited significant alterations in zebrafish 7-Ethoxyresorufin-O-deethylase activity at 3, 7, or 15 days of exposure. In addition, the significant increase in comet assay parameters of zebrafish hepatocytes in a concentration-dependent manner indicated BDE-47 and BDE-153 induced DNA damage, probably due to observed oxidative stress. Furthermore, a monotonically upregulation of p53 and Caspase3, which are apoptotic-regulated genes, and decreased expression ratio of the anti-apoptotic B-cell lymphoma/ leukaemia-2 and Bcl2-associated X protein genes for all BDE-47 and BDE-153 treatments at 7 and 15 days indicated apoptosis induction in zebrafish liver. Our findings help elucidate the mechanisms of BDE-47-and BDE-153-induced toxicity in zebrafish hepatocytes.

show abstract

Cellular metabolomics reveals glutamate and pyrimidine metabolism pathway alterations induced by BDE-47 in human neuroblastoma SK-N-SH cells

Cited by 22 publications

References 38 publications

Metabolomics-based study reveals the effect of lead (Pb) in the culture environment on Whitmania pigra

Metabolomics-based study reveals the effect of lead (Pb) in the culture environment on Whitmania pigra

Kunxian Capsule for Rheumatoid Arthritis: Inhibition of Inflammatory Network and Reducing Adverse Reactions Through Drug Matching

Hepatic oxidative stress, DNA damage and apoptosis in adult zebrafish following sub‐chronic exposure to BDE‐47 and BDE‐153

Contact Info

Product

Resources

About