Cellular metabolism network of Bacillus thuringiensis related to erythromycin stress and degradation

Zhou, Pulin; Ya, Chen; Lu, Qiping; Qin, Huaming; Ou, Huase; He, Bin; Ye, Jinshao

doi:10.1016/j.ecoenv.2018.05.048

Cited by 26 publications

(8 citation statements)

References 47 publications

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“…However, only a few microorganisms with erythromycin-degrading capabilities have been isolated from erythromycincontaminated areas, such as Pseudomonas aeruginosa, Pseudomonas putida, Bacillus thuringiensis, and Ochrobactrum sp. [12,13,19,20]. In this study, the colony characteristics of strain RJJ-61 were in accordance with a previous report of D. lacustris [21], and it was identified as a member of the genus Delftia based on phylogenetic analysis.…”

Section: Discussionsupporting

confidence: 90%

Biodegradation of erythromycin by Delftia lacustris RJJ‐61 and characterization of its erythromycin esterase

et al. 2020

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The residual erythromycin in fermentation waste can pollute the environment and threaten human health. However, there are no effective approaches to remedy this issue. In this study, an erythromycin‐degrading bacterium named RJJ‐61 was isolated and identified as a strain of Delftia lacustris based on morphological and phylogenetic analyses. The degradation ability of this strain was also evaluated; it could degrade 45.18% of erythromycin at 35°C in 120 h. Furthermore, the key degradation gene ereA was cloned from strain RJJ‐61 and expressed in Escherichia coli BL21; the molecular weight of the expressed protein was ~45 kDa. The enzyme activity of EreA was 108.0 mU ml−1 at 35°C and pH 7.0. Finally, the EreA protein was used to degrade erythromycin from mycelial dregs and 50% diluted solution, and the removal rates in them were 41.42% and 69.78%, respectively. In summary, D. lacustris RJJ‐61 is a novel erythromycin‐degrading strain that has great potential to remove erythromycin pollutants from the environment.

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Section: Discussionsupporting

confidence: 90%

Biodegradation of erythromycin by Delftia lacustris RJJ‐61 and characterization of its erythromycin esterase

et al. 2020

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“…In this work, the proteins associated with glycerophospholipid metabolism were located in the cytoplasm and belonged to the major intrinsic protein family (Table 2), which includes transmembrane protein channels, e.g., aquaporins, aquaglyceroporins, and S-aquaporins [50]. According to a previous study, after microorganisms sense a drug, phospholipid synthesis is accelerated for drug transport [51,52], suggesting that the AM applied to the E. coli O157:H7 may induce glycerophospholipid metabolism in the cytoplasm. Homologous recombination and DNA repair (Table 2) are involved in the response to stress and particularly the SOS response.…”

Section: Unique Enriched Biological Processes and Network Clustering Of Am-ckmentioning

confidence: 72%

Use of Acetic Acid to Partially Replace Lactic Acid for Decontamination against Escherichia coli O157:H7 in Fresh Produce and Mechanism of Action

Wang

Yue

et al. 2021

Foods

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Escherichia coli O157:H7 is frequently detected in ready-to-eat produce and causes serious food-borne diseases. The decontamination efficacy of lactic acid (LA) is clearly established. In this study, LA was mixed with acetic acid (AA) to reduce costs while achieving consistent or better inhibitory effects. Time-kill curves and inoculation experiments using fresh-cut spinach and arugula indicated that 0.8%LA+0.2%AA shows similar antibacterial effects to those of 1%LA. To determine whether 1%LA and 0.8%LA+0.2%AA exert antibacterial effects by similar mechanisms, proteomics analysis was used. The proteins related to macromolecule localization, cellular localization, and protein unfolding were uniquely altered after the treatment with 1%LA, and the proteins related to taxis, response to stress, catabolic process, and the regulation of molecular function were uniquely altered after the treatment with 0.8%LA+0.2%AA. Based on these findings, combined with the results of a network clustering analysis, we speculate that cell membrane damage is greater in response to LA than to 0.8%LA+0.2%AA. This prediction was supported by cell membrane permeability experiments (analyses of protein, nucleotide, ATP, and alkaline phosphatase leakage), which showed that LA causes greater membrane damage than 0.8%LA+0.2%AA. These results provide a theoretical basis for the application of an acid mixture to replace LA for produce decontamination.

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“…The glmU gene obviously forms an operon with the prs gene (CDIF630erm_03828) encoding ribose-phosphate pyrophosphokinase that catalyzes the conversion of ribose-5-phosphate into phosphoribosyl pyrophosphate during nucleotide biosynthesis. The prs gene was one of the major up-regulated genes of Bacillus thuringiensis in response to erythromycin treatment (Zhou et al, 2018). Both genes were found up-regulated under low iron conditions.…”

Section: Resultsmentioning

confidence: 99%

Iron Regulation in Clostridioides difficile

et al. 2018

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The response to iron limitation of several bacteria is regulated by the ferric uptake regulator (Fur). The Fur-regulated transcriptional, translational and metabolic networks of the Gram-positive, pathogen Clostridioides difficile were investigated by a combined RNA sequencing, proteomic, metabolomic and electron microscopy approach. At high iron conditions (15 μM) the C. difficile fur mutant displayed a growth deficiency compared to wild type C. difficile cells. Several iron and siderophore transporter genes were induced by Fur during low iron (0.2 μM) conditions. The major adaptation to low iron conditions was observed for the central energy metabolism. Most ferredoxin-dependent amino acid fermentations were significantly down regulated (had, etf, acd, grd, trx, bdc, hbd). The substrates of these pathways phenylalanine, leucine, glycine and some intermediates (phenylpyruvate, 2-oxo-isocaproate, 3-hydroxy-butyryl-CoA, crotonyl-CoA) accumulated, while end products like isocaproate and butyrate were found reduced. Flavodoxin (fldX) formation and riboflavin biosynthesis (rib) were enhanced, most likely to replace the missing ferredoxins. Proline reductase (prd), the corresponding ion pumping RNF complex (rnf) and the reaction product 5-aminovalerate were significantly enhanced. An ATP forming ATPase (atpCDGAHFEB) of the F0F1-type was induced while the formation of a ATP-consuming, proton-pumping V-type ATPase (atpDBAFCEKI) was decreased. The [Fe-S] enzyme-dependent pyruvate formate lyase (pfl), formate dehydrogenase (fdh) and hydrogenase (hyd) branch of glucose utilization and glycogen biosynthesis (glg) were significantly reduced, leading to an accumulation of glucose and pyruvate. The formation of [Fe-S] enzyme carbon monoxide dehydrogenase (coo) was inhibited. The fur mutant showed an increased sensitivity to vancomycin and polymyxin B. An intensive remodeling of the cell wall was observed, Polyamine biosynthesis (spe) was induced leading to an accumulation of spermine, spermidine, and putrescine. The fur mutant lost most of its flagella and motility. Finally, the CRISPR/Cas and a prophage encoding operon were downregulated. Fur binding sites were found upstream of around 20 of the regulated genes. Overall, adaptation to low iron conditions in C. difficile focused on an increase of iron import, a significant replacement of iron requiring metabolic pathways and the restructuring of the cell surface for protection during the complex adaptation phase and was only partly directly regulated by Fur.

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Cellular metabolism network of Bacillus thuringiensis related to erythromycin stress and degradation

Cited by 26 publications

References 47 publications

Biodegradation of erythromycin by Delftia lacustris RJJ‐61 and characterization of its erythromycin esterase

Biodegradation of erythromycin by Delftia lacustris RJJ‐61 and characterization of its erythromycin esterase

Use of Acetic Acid to Partially Replace Lactic Acid for Decontamination against Escherichia coli O157:H7 in Fresh Produce and Mechanism of Action

Iron Regulation in Clostridioides difficile

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