Cellular mechanisms of intestine regeneration in the sea cucumber,Holothuria glaberrima Selenka (Holothuroidea:Echinodermata)

García‐Arrarás, José E.; Estrada, Lourdes; Santiago, Roberto; Torres, Irma I.; Díaz‐Miranda, Lucy; Torres-Avillán, Ilia

doi:10.1002/(sici)1097-010x(19980701)281:4<288::aid-jez5>3.0.co;2-k

Cited by 149 publications

(225 citation statements)

References 19 publications

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“…We have previously shown that proliferation of luminal epithelial cells from the cloaca and esophagus, and migration into the intestinal primordium are the main events during lumen formation and that these events take place during the second week of regeneration (García-Arrará s et al, 1998;Quiñ ones et al, 2002). Therefore, it might be possible that the content released from the spherules is somehow regulating the luminal epithelium proliferation and migration.…”

Section: Spherulocyte Role During Echinoderm Regenerationmentioning

confidence: 99%

“…Animals were dissected on various days post evisceration. Further details of animal maintenance and dissection have been given previously (García-Arrará s et al, 1998, 1999.…”

Section: Experimental Procedures Animalsmentioning

confidence: 99%

“…The immunohistochemical techniques used have been described before (García-Arrará s et al, 1998, 1999. Briefly, tissues were fixed in either picric-acid formaldehyde (Zamboni) or 4% paraformaldehyde.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Evisceration is followed by a process of regeneration whose outcome is the formation of new organs. We have focused our studies on the regeneration of the intestine to understand the cellular and genetic mechanisms that are key to the regeneration process (García-Arrará s et al, 1998, 1999.…”

Section: Introductionmentioning

confidence: 99%

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Spherulocytes in the echinoderm Holothuria glaberrima and their involvement in intestinal regeneration

García‐Arrarás¹,

Schenk²,

Rodrígues‐Ramírez³

et al. 2006

Developmental Dynamics

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The holothuroid echinoderm Holothuria glaberrima can regenerate its intestine after a process of evisceration. Spherule-containing cells, the spherulocytes, appear to be associated with intestinal regeneration. We have used histochemistry and immunocytochemistry to characterize these cells and their role in the regeneration process. Spherulocytes are 10 -20 m in diameter with an acrocentric nucleus and spherule-like structures within their cytoplasm. They are found in the connective tissue of the intestine and mesentery of noneviscerated and regenerating animals. During the second week of regeneration, the number of spherulocytes in the regenerating intestine increases and a dramatic change in their morphology occurs. Together with the morphological change, the immunohistochemical labeling of the cells also changes; the antibodies not only recognize the spherule structures but also label the cellular cytoplasm in a more homogeneous pattern. Moreover, immunohistochemical labeling also appears to be dispersed within the extracellular matrix, suggesting that the cells are liberating their vesicular contents. Spherulocytes are found in other tissues of H. glaberrima, always associated with the connective tissue component. Our data strongly suggest that spherulocytes are involved in intestinal regeneration but their specific role remains undetermined. In summary, our data expand our knowledge of the cellular events associated with regeneration processes in echinoderms and provide for comparisons with similar processes in vertebrates.

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Section: Spherulocyte Role During Echinoderm Regenerationmentioning

confidence: 99%

“…Animals were dissected on various days post evisceration. Further details of animal maintenance and dissection have been given previously (García-Arrará s et al, 1998, 1999.…”

Section: Experimental Procedures Animalsmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Spherulocytes in the echinoderm Holothuria glaberrima and their involvement in intestinal regeneration

García‐Arrarás¹,

Schenk²,

Rodrígues‐Ramírez³

et al. 2006

Developmental Dynamics

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“…The phenomenon of visceral regeneration was studied more exstensively in holothuroids, since the first half of the 20th century [31][32][33][34][35], and in asteroids by Anderson [36][37][38]. With regard to visceral regeneration in echinoderms, the most controversial topic has been the origin of the luminal epithelium.…”

Section: Introductionmentioning

confidence: 99%

Visceral regeneration in the crinoid Antedon mediterranea: basic mechanisms, tissues and cells involved in gut regrowth

et al. 2006

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Abstract:Crinoids are able to regenerate completely many body parts, namely arms, pinnules, cirri, and also viscera, including the whole gut, lost after self-induced or traumatic mutilations. In contrast to the regenerative processes related to external appendages, those related to internal organs have been poorly investigated. In order to provide a comprehensive view of these processes, and of their main events, timing and mechanisms, the present work is exploring visceral regeneration in the feather star Antedon meditteranea. The histological and cellular aspects of visceral regeneration were monitored at predetermined times (from 24 hours to 3 weeks post evisceration) using microscopy and immunocytochemistry. The overall regeneration process can be divided into three main phases, leading in 3 weeks to the reconstruction of a complete functional gut. After a brief wound healing phase, new tissues and organs develop as a result of extensive cell migration and transdifferentiation. The cells involved in these processes are mainly coelothelial cells, which after trans-differentiating into progenitor cells form clusters of enterocytic precursors. The advanced phase is then characterized by the growth and differentiation of the gut rudiment. In general, our results confirm the striking potential for repair (wound healing) and regeneration displayed by crinoids at the organ, tissue and cellular levels.

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Regeneration of the enteric nervous system in the sea cucumberHolothuria glaberrima

et al. 1999

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Among higher metazoans, echinoderms exhibit the most impressive capacity for regeneration. Holothurians, or sea cucumbers, respond to adverse stimuli by autotomizing and ejecting their visceral organs, which are then regenerated. Neuronal fibers and cell bodies are present within the viscera, but previous regeneration studies have not accounted for the nervous component. We used light microscopic immunocytochemistry and ultrastructural studies to describe the regeneration of the enteric nervous system in the sea cucumber Holothuria glaberrima. This study provides evidence that the enteric nervous system of this echinoderm regenerates after evisceration and that in 3–5 weeks the regenerated system is virtually identical to that of noneviscerated animals. The regeneration of the enteric nervous system occurs parallel to the regeneration of other organ components. Nerve fibers and cells are observed within the mesenterial thickenings that give rise to the new intestine and within the internal connective tissue prior to lumen formation. We also used bromodeoxyuridine incorporation to show that proliferation of the neuronal population occurs in the regenerating intestine. The regeneration of the nervous system commands high interest because members of the closely related phylum Chordata either lack or have a very limited capacity to regenerate their nervous system. Thus, holothurians provide a model system to study enteric nervous system regeneration in deuterostomes. J. Comp. Neurol. 406:461–475, 1999. © 1999 Wiley‐Liss, Inc.

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Cellular mechanisms of intestine regeneration in the sea cucumber,Holothuria glaberrima Selenka (Holothuroidea:Echinodermata)

Cited by 149 publications

References 19 publications

Spherulocytes in the echinoderm Holothuria glaberrima and their involvement in intestinal regeneration

Spherulocytes in the echinoderm Holothuria glaberrima and their involvement in intestinal regeneration

Visceral regeneration in the crinoid Antedon mediterranea: basic mechanisms, tissues and cells involved in gut regrowth

Regeneration of the enteric nervous system in the sea cucumberHolothuria glaberrima

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