Cellular Mechanisms in the Actions of Antiglaucoma Drugs

Yorio, Thomas

doi:10.1089/jop.1985.1.397

Cited by 18 publications

(9 citation statements)

References 110 publications

(99 reference statements)

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“…However, levels of lysophosphatidycholines were several times higher in injured corneal AQH samples in comparison with control AQH samples, suggesting that lysoPLD is released from corneal tissues into the AQH [45]. In addition, phosphatidylinositol (PIs) are one of the known classes of phospholipids undergoing significant changes during the administration of PG analogs for IOP lowering [46, 47]. Recent studies have also shown a significant difference in phospholipid species between human POAG and control trabecular meshwork tissue [48], yet the differences in phospholipid profiles in POAG AQH and control AQH have yet to be studied in further detail.…”

Section: Discussionmentioning

confidence: 99%

Phospholipid profiles of control and glaucomatous human aqueous humor

et al. 2014

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To compare phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and phosphatidylinositol) profiles of human control and glaucomatous aqueous humor (AQH). AQH samples were procured during surgery from human POAG and control subjects (n=15 each). Samples were used following institutional review board approved protocols and adhering to the tenets of the Declaration of Helsinki. Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford’s method, and select samples were confirmed with Densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ Quantum Access Max triple quadrupole mass spectrometer utilizing precursor ion scan (PIS) or neutral ion loss scan (NLS) using appropriate class specific lipid standards in a two step quantification process. The comparative profiles of phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines and phosphatidylinositols between control and glaucomatous AQH showed several species common between them. A number of unique lipids in all four phospholipid classes were also identified in control eyes that were absent in glaucomatous eyes and vice versa. A number of phospholipids were found to be uniquely present in control, but absent in glaucomatous AQH and vice versa. Compared with a previous study of control and POAG red blood cells, a number of these phospholipids are absent locally (AQH).

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Section: Discussionmentioning

confidence: 99%

Phospholipid profiles of control and glaucomatous human aqueous humor

et al. 2014

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“…24 PIs is one of the known classes of phospholipids that undergoes significant changes during the administration of PG analogs for IOP lowering. 25,26 Endogenous lipids in the anterior chamber may be involved in regulation of aqueous outflow, outflow facility, and IOP. The previous techniques, such as various forms of chromatography and nuclear magnetic resonance, necessitated knowledge of several different chemistries due to different chemical behavior of different lipid species even within a class, and the requirement of large amounts (microgram quantities) of lipids for identification and characterization.…”

Section: Discussionmentioning

confidence: 99%

Comparative Phospholipid Profiles of Control and Glaucomatous Human Trabecular Meshwork

Aribindi¹,

Guerra²,

Lee³

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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“…It has also been shown that the adenylcyclase system plays an important role in regulating aqueous humor formation (see 8,18).…”

Section: Modelmentioning

confidence: 99%

Aerobic and Anaerobic Metabolism of Bovine Ciliary Process: Effects of Metabolic and Transport Inhibitors

Braunagel¹,

Yorio²

1987

Journal of Ocular Pharmacology and Therapeutics

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In the present study we have measured the oxygen consumption and lactic acid production, under aerobic and anaerobic conditions, in the bovine ciliary process epithelium (CPE) in the presence and absence of transport modifiers.Basal oxygen consumption was 8-15 ul 02 consumed/mg protein/hr and decreased by 35% when sodium was removed or ouabain was added to the media. Anaerobic metabolism as measured by lactate production was also attenuated by sodium-free or ouabain treatment. When 0« consumption was severely limited by cyanide, lactic acid production increased significantly ("Pasteur effect"), whereas 2-deoxyglucose reduced lactate formation. Both chloride-free and acetazolamide treated CPE increased their dependency on aerobic glycolysis, and this response was also observed under anaerobic conditions, suggesting the presence of an anion transport mechanism.A net lactate production was also found to occur across the aqueous epithelium under aerobic and anaerobic conditions. These results are consistent with the presence of a bicarbonatesensitive anion transport system located in the ciliary process epithelium.

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Cellular Mechanisms in the Actions of Antiglaucoma Drugs

Cited by 18 publications

References 110 publications

Phospholipid profiles of control and glaucomatous human aqueous humor

Phospholipid profiles of control and glaucomatous human aqueous humor

Comparative Phospholipid Profiles of Control and Glaucomatous Human Trabecular Meshwork

Aerobic and Anaerobic Metabolism of Bovine Ciliary Process: Effects of Metabolic and Transport Inhibitors

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