Cellular Mechanisms During Mammalian Fertilization

Wassarman, Paul M.; Florman, Harvey M.

doi:10.1002/cphy.cp140124

Cited by 4 publications

(3 citation statements)

References 637 publications

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“…However, it is now generally accepted that ZP3 is the natural agonist that initiates the acrosome reaction upon binding of acrosome-intact sperm to the ZP [Bleil and Wassarman, 1983;Wassarman, 1995;Darszon et al, 1996;Wassarman and Florman, 1997;Florman et al, 1998;Nixon et al, 2001]. Criteria now are available that permit one to distinguish between the, so-called, 'spontaneous' acrosome reaction and the ZP3-induced acrosome reaction (e.g., sensitivity to pertussis toxin).…”

Section: Mouse Zp3 Is An Acrosome Reaction Inducermentioning

confidence: 99%

Towards the Molecular Basis of Sperm and Egg Interaction during Mammalian Fertilization

Wassarman

Litscher²

2000

Cells Tissues Organs

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During the past 2 decades, a number of genes have been cloned from mammals which encode polypeptides that participate in the process of fertilization. Among these are glycoproteins ZP1–3 that constitute the zona pellucida of eggs from mice to human beings. In mice, one of these glycoproteins, mZP3, acts as a primary sperm receptor and acrosome reaction-inducer. The evidence suggests that acrosome-intact sperm recognize and bind to a specific class of mZP3 oligosaccharides present on two serine residues (O-linked) located near the carboxy-terminus of the polypeptide. Mutagenesis of either of these residues results in the synthesis of an inactive form of the receptor. Therefore, mammalian fertilization is a carbohydrate-mediated event. It is possible that changes in the structure of these oligosaccharides (e.g., composition, sequence, linkages, modifications, etc.) could account for species-specific binding of sperm to eggs. Stably transfected somatic cells, null mutant animals, and DNA constructs are now available to test this possibility both in vivo and in vitro.

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Section: Mouse Zp3 Is An Acrosome Reaction Inducermentioning

confidence: 99%

Towards the Molecular Basis of Sperm and Egg Interaction during Mammalian Fertilization

Wassarman

Litscher²

2000

Cells Tissues Organs

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“…The microanatomy of the koala ovary is fundamentally similar to that described for other marsupials [18][19][20][21][22][23] and eutherian mammals [24][25][26][27]. A central medulla containing blood vessels, nerves, and connective tissue is surrounded by a cortex in which various stages of germ cell development (primordial, primary, secondary, and tertiary follicles), interstitial tissue, and depending on the stage of the cycle, a functional or degenerating corpus luteum are found.…”

Section: Discussionmentioning

confidence: 65%

Anatomy of the Female Koala Reproductive Tract

Pagliarani,

Palmieri,

McGowan

et al. 2023

Biology

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The koala (Phascolarctos cinereus), while being an iconic Australian marsupial, has recently been listed as endangered. To establish an improved understanding of normal reproductive anatomy, this paper brings together unpublished research which has approached the topic from two perspectives: (1) the establishment of an artificial insemination program, and (2) the definition of Chlamydia spp.-derived histopathological changes of the female koala urogenital system. Based on the presentation and histological processing of over 70 opportunistic specimens, recovered from wildlife hospitals in Southeast Queensland (Australia), we describe the gross and microanatomy of the koala ovary, oviduct, uteri, vaginal complex, and urogenital sinus during the interestrous, proliferative, and luteal phases of the reproductive cycle.

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“…The egg complex following ovulation enters the oviduct consisting of three components: i) the oocyte (egg) arrested at metaphase of meiosis II; ii) the extracellular matrix of the egg or zona pellucida consisting of three glycoproteins (ZP1, ZP2 and ZP3), synthesized and secreted by the oocyte; iii) the cumulus oophorus, consisting of granulosa cells enriched with hyaluronic acid ( 1 , 2 ). Cumulus cells support fertilization, and in vitro fertilization can be achieved more efficiently with them than without them ( 3 – 5 ).…”

Section: Introductionmentioning

confidence: 99%

The molecular basis of fertilization (Review)

Georgadaki

Khoury

Spandidos

et al. 2016

International Journal of Molecular Medicine

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Fertilization is the fusion of the male and female gamete. The process involves the fusion of an oocyte with a sperm, creating a single diploid cell, the zygote, from which a new individual organism will develop. The elucidation of the molecular mechanisms of fertilization has fascinated researchers for many years. In this review, we focus on this intriguing process at the molecular level. Several molecules have been identified to play a key role in each step of this intriguing process (the sperm attraction from the oocyte, the sperm maturation, the sperm and oocyte fusion and the two gamete pronuclei fusion leading to the zygote). Understanding the molecular mechanisms of the cell-cell interactions will provide a better understanding of the causes of fertility issues due to fertilization defects.

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Cellular Mechanisms During Mammalian Fertilization

Cited by 4 publications

References 637 publications

Towards the Molecular Basis of Sperm and Egg Interaction during Mammalian Fertilization

Towards the Molecular Basis of Sperm and Egg Interaction during Mammalian Fertilization

Anatomy of the Female Koala Reproductive Tract

The molecular basis of fertilization (Review)

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