Cellular Magnetic Resonance Imaging: In Vivo Imaging of Melanoma Cells in Lymph Nodes of Mice

Foster, Paula J.; Dunn, Elizabeth; Karl, Kristina E; Snir, Jonatan; Nycz, Colleen M.; Harvey, Alfred; Pettis, Ron J

doi:10.1593/neo.07937

Cited by 58 publications

(62 citation statements)

References 45 publications

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“…These include detection of iron-labeled hepatocytes, macrophages, dendritic, cancer, and stem cells using T1-, T2-, and T2*-weighted imaging techniques and quantification using multiple MRI parameters including T2, R2, and R2* (20,(28)(29)(30)(31)(32)(33)(34)(35). It has been established qualitatively that the number of MPIO-labeled human breast cancer cells delivered to the brain is associated with the number of MRI signal voids (18).…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

et al. 2009

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The metastatic cell population, ranging from solitary cells to actively growing metastases, is heterogeneous and unlikely to respond uniformly to treatment. However, quantification of the entire experimental metastatic cell population in whole organs is complicated by requirements of an imaging modality with the large field of view and high spatial resolution necessary to detect both single cells and metastases in the same organ. Thus, it is difficult to assess differential responses of these distinct metastatic populations to therapy. Here, we develop a magnetic resonance imaging (MRI) technique capable of quantifying the full population of metastatic cells in a secondary organ. B16F1 mouse melanoma cells were labeled with micron-sized iron oxide particles (MPIO) and injected into mouse liver via the mesenteric vein. Livers were removed immediately or at day 9 or 11, following doxorubicin or vehicle control treatment, and imaged using a 3T clinical magnetic resonance scanner and custom-built gradient coil. Both metastases (>200 μm) and MPIO-labeled single cells were detected and quantified from MR images as areas of hyperintensity or hypointensity (signal voids), respectively. We found that 1 mg/kg doxorubicin treatment inhibited metastasis growth (n = 11 per group; P = 0.02, t test) but did not decrease the solitary metastatic cell population in the same livers (P > 0.05). Thus, the technique presented here is capable of quickly quantifying the majority of the metastatic cell population, including both growing metastases and solitary cells, in whole liver by MRI and can identify differential responses of growing metastases and solitary cells to therapy. [Cancer Res 2009;69(21):8326-31]

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Section: Discussionmentioning

confidence: 99%

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

et al. 2009

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“…The majority of research in this area has utilized MNP because of their greater sensitivity compared to Gad-based agents (51,52). In experimental cellular MRI, cells are typically loaded with MNP prior to their injection or implantation (53)(54)(55)(56)(57)(58)(59). The presence of this magnetic label in cells causes a distortion in the magnetic field and leads to signal hypointensities (negative contrast) in images sensitive to this change (T2-or T2*-weighted images).…”

Section: Technological Advances: Magnetic Resonance Imaging As a Toolmentioning

confidence: 99%

New clinical and experimental approaches for studying tumor dormancy: does tumor dormancy offer a therapeutic target?

Goss

Allan

Rodenhiser

et al. 2008

Apmis

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Goss P, Allan AL, Rodenhiser DI, Foster PJ, Chambers AF. New clinical and experimental approaches for studying tumor dormancy: does tumor dormancy offer a therapeutic target ? APMIS 2008;116:552-68. Tumor dormancy is a significant clinical problem. Primary treatment of a cancer may be apparently successful, and yet the tumor may recur either locally or as distant metastases years or even decades later. The ability to predict which patients are likely to develop recurrences is imprecise, relying on probabilities of recurrence based on features of the primary cancer. This uncertainty presents clinical challenges regarding who to treat and how, in order to prevent recurrence after periods of dormancy. Recent clinical trials in breast cancer support the idea that some patients may harbor tumor cells that are capable of forming late-developing metastases years after removal of the primary tumor, and that these dormant cancer cells may in some cases be effectively treated with long-term therapy. Advances in experimental studies of tumor dormancy are shedding light on the nature of dormancy, and are providing both new technologies and conceptual approaches for studying tumor dormancy. A better understanding of mechanisms responsible for tumor dormancy and recurrence will be important for improving care of patients at risk for late-developing metastases.

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“…The MRI image ( Figure 3a) was found to reflect precisely the actual morphology and tumor depth (Figure 3b tissue and also provided a 3-D view of the state of infiltration, useful for deciding on a resection area prior to surgery. MRI has also been used to image nodal and distant metastasis in a murine model (Foster et al, 2008) as well as different organs in humans (King, 2004). However, the availability and cost of MRI is a limiting factor.…”

Section: T Category Classificationmentioning

confidence: 99%

Determination of Melanoma Lateral and Depth Margins: Potential for Treatment Planning and Five-Year Survival Rate

Wang¹,

Qiu²,

Milner³

2011

Skin Cancer Overview

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Cellular Magnetic Resonance Imaging: In Vivo Imaging of Melanoma Cells in Lymph Nodes of Mice

Cited by 58 publications

References 45 publications

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

New clinical and experimental approaches for studying tumor dormancy: does tumor dormancy offer a therapeutic target?

Determination of Melanoma Lateral and Depth Margins: Potential for Treatment Planning and Five-Year Survival Rate

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