Cellular localization of TWIK-1, a two-pore-domain potassium channel in the rodent inner ear

Nicolas, Marie‐Thérèse; Barhanin, Jacques; Reyes, Roberto V.; Demêmes, Danielle

doi:10.1016/s0378-5955(03)00162-x

Cited by 20 publications

(14 citation statements)

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“…Previous RT-PCR and in situ hybridization studies have revealed that TWIK-1 is present in the mammalian brain and also in a wide variety of tissues outside the nervous system (Lesage et al, 1996;Orias et al, 1997, Arrighi et al, 1998 Wang et al,1998;Medhurst et al, 2001 andTalley et al, 2001). TWIK-1 expression has been previously shown in the cochlear nucleus using PCR (Holt et al, 2006) and in the inner ear and cochlear nucleus using in situ hybridization (Nicolas MT et al, 2003;Talley et al, 2001). In the present study, a decrease in TWIK-1 expression was found which did not reach significance until 3 weeks following deafening (24% decrease, p=0.003) and remained significantly decreased by 20% (p=0.002) at 3 months following deafness (Figure 2A).…”

Section: Pcr Resultssupporting

confidence: 52%

Deafness associated changes in two-pore domain potassium channels in the rat inferior colliculus

et al. 2007

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Two-pore potassium channels can influence neuronal excitability by regulating background leakage of potassium ions and resting membrane potential. The present study used quantitative real time PCR and in situ hybridization to determine if the decreased activity from deafness would induce changes in two-pore potassium channel subunit expression in the rat inferior colliculus. Ten subunits were assessed with qRT-PCR at 3 days, 3 weeks and 3 months following bilateral cochlear ablation. TASK-1, TASK-5 and THIK-2 showed significant decreases in expression at all three times assessed. TASK-5, relatively specific to auditory neurons, had the greatest decrease. TWIK-1 was significantly decreased at 3 weeks and 3 months following deafness and TREK-2 was only significantly decreased at 3 days. TASK-3, TWIK-2, THIK-1, TRAAK and TREK-1 did not show any significant changes in gene expression. In situ hybridization was used to examine TASK-1, TASK-5, TWIK-1 and THIK-2 in the central nucleus, dorsal cortex and lateral (external) cortex of the IC in normal hearing animals and at 3 weeks following deafening. All four subunits showed expression in neurons throughout IC subdivisions in normal hearing rats, with TASK-5 having the greatest overall number of labeled neurons. There was no co-localization of subunit expression with GFAP immunostaining, indicating no expression in glia. Three weeks following deafening there was a significant decrease in the number of neurons expressing TASK-1 and THIK-2 in the IC, while TASK-5 had significant decreases in the central nucleus and dorsal cortex and TWIK-1 in the lateral and dorsal cortices. Two-pore potassium channels (K 2p ) are a class of open rectifying potassium selective channels (Ketchum et al., 1995) that, when activated, allow a background leakage of potassium ions that raises the resting membrane potential to hyperpolarizing levels, resulting in decreased neuronal excitability (see Lesage and Lazdunski, 2000;Goldstein et al., 2001;Patel and Honore, 2001; Plant et al., 2005 for reviews). There are, to date, 18 subunits in the K 2p channel family that have been divided into different classes based on what is know about their sensitivities. The TASK-1 (K 2p 3.1, KCNK3), TASK-3 (K 2p 9.1, KCNK9) and TWIK-1 (K 2p 1.1, KCNK1) subunits are widely expressed throughout the brain but have been reported to have only moderate expression in the auditory brain stem Talley et al., 2001). The TASK-5 (K 2p 15.1, KCNK15) subunit has a relatively selective expression, primarily found in Author for correspondence: Dr. Richard A. Altschuler, KHRI, Department of Otolaryngology, The University of Michigan, 1301 East Ann Street, Ann Arbor, MI, Tel: (734) Fax: (734) 764-0014, E-mail: E-mail: shuler@umich.edu. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof befo...

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Section: Pcr Resultssupporting

confidence: 52%

Deafness associated changes in two-pore domain potassium channels in the rat inferior colliculus

et al. 2007

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“…Sections were collected in cold 0.1 M PBS. Immunocytochemical analysis was carried out as previously described (Nicolas et al, 2003). BrieXy, free-Xoating sections were preincubated for 2 h under gentle rotation at 4°C in a blocking serum containing 10% normal donkey serum and 0.3% Triton X-100 in PBS.…”

Section: Immunocytochemical Methodsmentioning

confidence: 99%

Immunocytochemical and pharmacological characterization of metabotropic glutamate receptors of the vestibular end organs in the frog

et al. 2005

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“…In mouse tissues, TWIK-1 is expressed highly in the brain, lung and kidney [2]. In rodents, TWIK-1 mRNA is also expressed in the non-sensory cells of the inner ear [3]. Human TWIK-2 is 54% identical in amino acid sequence to TWIK-1 and is expressed in many peripheral tissues such as A predicted membrane topology of a K 2P channel shows 2P domains and 4TM segments.…”

Section: Twik Family (Twik-1 and Twik-2)mentioning

confidence: 99%

Physiology and Pharmacology of Two-Pore Domain Potassium Channels

Kim

2005

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135

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Searching the DNA database has led to the identification of a class of K+ channels now referred to as two-pore or tandem-pore domain K+ (K2P) channels. The K2P channel is structurally unique in that each subunit possesses two pore-forming domains and four transmembrane segments. In mammals, sixteen K2P channel genes have been identified, and their mRNA transcripts are expressed in many different cell types and tissues. K2P channels have properties of background or leak K+ channels, and therefore play a crucial role in setting the resting membrane potential and regulating cell excitability. Some K2P channels are activated by certain physical and chemical factors such as lipids, volatile anesthetics, heat, oxygen, protons and membrane tension. Some K2P channels are targets of agonists that bind receptors coupled to different types of G proteins, and are probably involved in a variety of neurotransmitter and peptide hormone-mediated signal transduction processes. Such diverse properties of K2P channels suggest that they are involved in many different physiological and pathophysiological processes. Therefore, K2P channels could become potentially important therapeutic targets for the treatment of various pathological conditions.

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Cellular localization of TWIK-1, a two-pore-domain potassium channel in the rodent inner ear

Cited by 20 publications

References 24 publications

Deafness associated changes in two-pore domain potassium channels in the rat inferior colliculus

Deafness associated changes in two-pore domain potassium channels in the rat inferior colliculus

Immunocytochemical and pharmacological characterization of metabotropic glutamate receptors of the vestibular end organs in the frog

Physiology and Pharmacology of Two-Pore Domain Potassium Channels

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