Cellular localization of corticotropin releasing factor receptors in the adult mouse cerebellum

Bishop, Georgia A.; Seelandt, C.M; King, James S.

doi:10.1016/s0306-4522(00)00413-9

Cited by 51 publications

(54 citation statements)

References 38 publications

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“…The GFAP antibody labeled only cells that were consistent with fibrillary astrocytes, based on morphology and distribution ( Fig. 5; Bishop et al, 2000;Debus et al, 1983).…”

Section: Primary Antibodiesmentioning

confidence: 99%

Type III adenylyl cyclase localizes to primary cilia throughout the adult mouse brain

Bishop

Berbari

Lewis

et al. 2007

J of Comparative Neurology

281

271

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Solitary primary cilia project from nearly every cell type in the human body. These organelles are considered to have important sensory and signaling functions. Although primary cilia have been detected throughout the mammalian brain, their functions are unknown. The study of primary cilia in the brain is constrained by the scarcity of specific markers for these organelles. We previously demonstrated that type III adenylyl cyclase (ACIII) is a marker for primary cilia on neonatal hippocampal neurons in vivo and in vitro. We further showed that ACIII localizes to cilia on cultured glial cells. Here, we report that ACIII is a marker for primary cilia throughout many regions of the adult mouse brain. Furthermore, we report that ACIII localizes to primary cilia on choroid plexus cells and some astrocytes in the brain, which to our knowledge is the first report of a marker for visualizing cilia on glia in vivo. Overall, our data indicate that ACIII is a prominent marker of primary cilia in the brain and will provide an important tool to facilitate further investigations into the functions of these organelles.

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“…The GFAP antibody labeled only cells that were consistent with fibrillary astrocytes, based on morphology and distribution ( Fig. 5; Bishop et al, 2000;Debus et al, 1983).…”

Section: Primary Antibodiesmentioning

confidence: 99%

Type III adenylyl cyclase localizes to primary cilia throughout the adult mouse brain

Bishop

Berbari

Lewis

et al. 2007

J of Comparative Neurology

281

271

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“…A series of electrophysiological and immunocytochemical studies examining rat (Swinny, et al 2003;Swinny et al 2004) and mouse (King and Bishop 2002;Bishop, 2002;Bishop, et al 2000) cerebellum have concluded that CRH 1 and CRH 2 are expressed differentially in preand post-synaptic elements. Furthermore, Swinny et al (Swinny et al 2003) concluded that CRH 2 is membrane bound at synapses, while CRH 1 is not.…”

Section: Cerebellummentioning

confidence: 99%

Synaptic physiology of central CRH system

Gallagher

Orozco-Cabal

Liu

et al. 2008

European Journal of Pharmacology

153

123

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Corticotropin Releasing Hormone (CRH) or Corticotropin Releasing Factor (CRF) and its family of related naturally occurring endogenous peptides and receptors are becoming recognized for their actions within central (CNS) and peripheral (PNS) nervous systems. It should be recognized that the term 'CRH' has been displaced by 'CRF' (Guillemin 2005). However, to maintain uniformity among contributions to this special issue we have used the original term, CRH. The term 'CRF' has been associated recently with CRH receptors and designated with subscripts by the IUPHAR nomenclature committee (Hauger R.L. et al. 2003) to denote the type and subtype of receptors activated or antagonized by CRH ligands. CRH, as a hormone, has long been identified as the regulator of basal and stress-induced ACTH release within the hypothalamo-pituitary-adrenal axis (HPA axis). But the concept, that CRH and its related endogenous peptides and receptor ligands have non-HPA axis actions to regulate CNS synaptic transmission outside the HPA axis, is just beginning to be recognized and identified . It is especially noteworthy that since the synapse has become a prime focus for a variety of mental diseases, e.g. schizophrenia (Fischbach 2007), and neurological disorders, e.g., Alzheimer's disease (Bell and Cuello 2006), we suggest that "THE STRESSED SYNAPSE" has been overlooked (c.f., Kim and Diamond 2002; Radley and Morrison 2005) as a major contributor to many CNS disorders. We present data demonstrating CRH neuroregulatory and neuromodulatory actions at three limbic synapses, the basolateral amygdala to central amygdala synapse; the basolateral amygdala to medial prefrontal cortex synapse, and the lateral septum mediolateral nucleus synapse. A novel stress circuit is presented involving these three synapses. We suggest that CRH ligands and their receptors are significant etiological factors that need to be considered in the pharmacotherapy of mental diseases associated with CNS synaptic transmission.

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“…which contains GPI-anchored cell-adhesion molecules (Rodriguez-Boulan and Powell, 1992) and receptors for neurotransmitters (Pan, 2001) and hormones (Bishop et al, 2000). In simple epithelial cells two plasma membrane domains with a distinct protein and lipid composition are separated by tight junctions: the apical membrane acting as a barrier towards the external milieu (lumen) and the basolateral membrane facing neighbouring cells and the extracellular matrix (Eaton and Simons, 1995).…”

mentioning

confidence: 99%

Polarity and lipid raft association of the components of the ciliary neurotrophic factor receptor complex in Madin-Darby canine kidney cells

Buk

Waibel

Braig

et al. 2004

Journal of Cell Science

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Ciliary neurotrophic factor (CNTF) signals via a tripartite receptor complex consisting of the glycosyl-phosphatidylinositol (GPI)-anchored CNTF receptor (CNTF-R), the leukaemia inhibitory factor receptor (LIF-R) and the interleukin-6 (IL-6) signal transducer gp130. We have recently reported that gp130 is endogenously expressed in the polarised epithelial model cell line Madin-Darby canine kidney (MDCK) and we have demonstrated a preferential basolateral localisation of this protein. In the present study we show that MDCK cells also express the LIF-R and respond to stimulation with human LIF by activation of tyrosine phosphorylation of signal transducer and activator of transcription-3 (STAT3), both however in an unpolarised fashion. This suggests that MDCK cells may be target cells for LIF. We have furthermore stably expressed the human CNTF-R in MDCK cells and by two different assays we found an apical localisation. Consistent with these findings, stimulation of CNTF-R-positive cells resulted only in an activation of STAT3 when CNTF was added apically. These data demonstrate that each subunit of the CNTF receptor complex has a distinct distribution in polarised cells which may reflect the different roles the respective cytokines play in vivo.Since it is currently believed that lipid rafts are involved in signal transduction as well as protein sorting we studied the association of the three receptor complex components with membrane rafts using different protocols. Whereas the CNTF-R cofractionated quantitatively with lipid rafts independently of the method used, gp130 and the LIF-R were found to associate with lipid rafts only partially when detergents were used for isolation. These findings could indicate that either the three receptor complex subunits are localised to the same kind of raft but with different affinities to the liquid-ordered environment, or that they are localised to different types of rafts. CNTF-, LIF-, and IL-6-dependent STAT3 activation was sensitive to the cholesterol-depleting drug methyl-β-cyclodextrin (MCD) suggesting that the integrity of lipid rafts is important for IL-6-type cytokine-induced STAT activation.

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Cellular localization of corticotropin releasing factor receptors in the adult mouse cerebellum

Cited by 51 publications

References 38 publications

Type III adenylyl cyclase localizes to primary cilia throughout the adult mouse brain

Type III adenylyl cyclase localizes to primary cilia throughout the adult mouse brain

Synaptic physiology of central CRH system

Polarity and lipid raft association of the components of the ciliary neurotrophic factor receptor complex in Madin-Darby canine kidney cells

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