Cellular Localization of Anti-DNP-PLL and Anticonveyor Albumin Antibodies in Genetic Nonresponder Guinea Pigs Immunized With DNP-PLL Albumin Complexes

Abstract

About 30-40% of random-bred Hartley (I) and all strain 2 (2) guinea pigs recognize poly-L-lysine (PLL) I and hapten-PLL conjugates as antigens. These "responder" animals develop delayed hypersensitivity to the conjugated polymer and produce specific antihapten antibodies when immunized with hapten-PLL conjugates. The ability to respond to these antigens was shown to be controlled by a dominant autosomal gene (2, 3). Hapten-PLL conjugates which are complete antigens only for responder animals can behave as haptens in genetic nonresponder guinea pigs. Thus, insoluble electrostatic complexes formed by the reaction of positively charged 2,4-dinitrophenyl-PLL (DNP-PLL) with negatively charged foreign albumins, acting as conveyor or Schlepper molecules, induce the formation of high serum levels of anti-DNP antibodies in nonresponder guinea pigs (4). These animals, however, in contrast with responder guinea pigs, do not show delayed hypersensitivity reactions to DNP-PLL, which is not surprising, since it is well known that unconjugated haptens cannot elicit delayed hypersensitivity reaction (5). In these systems, the recognition of antigenicity and the capacity to show delayed hypersensitivity reactions therefore involve some identical immunological recognition mechanism in which the specificity of the carrier molecule plays an important part. This relationship is further illustrated by the observations of Schlossman on the ez DNP-oligo-L-lysines that the same minimum number of 7-8 lysine residues is essential both for immunization of responder guinea pigs and for the elicitafion of delayed hypersensitivity reactions in these animals (6, 7).An understanding of the immune response to hapten-protein and hapten-polypeptide conjugates must depend upon a better knowledge of the hapten-carrier rela-*

Specificity of Antibodies Synthetized by Immunocytes as Detected by Immunoenzyme Techniques

“…The results of the present work are in good agreement with those of other studies [1,2,4,5,11,21] and support the concept of the by Immunocytes as Detected by Immunoenzymc Techniques 169 narrow specificity of the antibody synthesized by a single immunocyte and indicate that an immunologically competent cell, once engaged in the synthesis of a specific antibody, produces antibody of only this specificity. In the studies referred to above, about 3% mixed cells were noted.…”

Hapten Carrier Relationships in the DNP-Pll·foreign Albumin Complex System: Induction of Tolerance and Stimulation of Cells in Vitro

“…Unpublished observations. separate cells (24). Indeed, the anti-DNP-PLL antibodies produced by responder and nonresponder guinea pigs have the same degree of PLL specificity as judged by fluorescence quenching titrations with model ligands (25,4).…”

When All Is Said And Done..

“…Moreover, technologies by then available used a combination of immunofluorescence and historadi ography to identify the specificity of antibodies produced by individual plasma cells in animals immunized with hapten-protein conjugates. With Ira Green and Pierre Vassalli, we were delighted to find that the antibody producing cells of animals immunized with DNP-BSA or DNP-BGG produced antibodies specific for either the hapten or the carrier molecule, but never reactive with both, in spite of the presence of both determinants on the immunizing antigen (38,39). At about the same time Makela came to the identical conclusion by a different approach (40).…”