Cellular Localization and Regulation of Expression of the PLET1 Gene in Porcine Placenta

Teng, Liu; Hong, Linjun; Liu, Ruize; Chen, Ran; Li, Xinyun; Yu, Mei

doi:10.3390/ijms17122048

Cited by 8 publications

(5 citation statements)

References 47 publications

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“…Therefore, six types of samples (3 compartments per gestational day) were used for RNA-sequencing and a total of 15,062 genes were detected. Genes that have been reported to be expressed in pig endometrial luminal epithelium (such as SPP1 (Secreted phosphoprotein 1) [ 27 , 28 ], MUC1 (Mucin 1) [ 29 ] and STC1 (Stanniocalcin 1) [ 30 ]) were included, and pig conceptus-expressed genes including IFNG (Interferon gamma) [ 31 ], PLET1 (Placenta expressed transcript 1) [ 32 ], PLAC1 (Placenta-specific 1) [ 33 ] and PLAC8 (Placenta-specific 8) [ 34 , 35 ] were also detected. Descriptive statistics of the RNA-seq data are given in Table S1 .…”

Section: Resultsmentioning

confidence: 99%

Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs

Wang

Zhao

Deng

et al. 2021

IJMS

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Attachment of conceptus to the endometrial luminal epithelium (LE) is a critical event for early placentation in Eutheria. Since the attachment occurs at a particular site within the uterus, a coordinated communication between three spatially distinct compartments (conceptus and endometrial LE from two anatomical regions of the uterus to which conceptus attaches and does not attach) is essential but remains to be fully characterized. Using the laser capture microdissection (LCM) technique, we firstly developed an approach that can allow us to pair the pig conceptus sample with its nearby endometrial epithelium sample without losing the native spatial information. Then, a comprehensive spatio-temporal transcriptomic profile without losing the original conceptus-endometrium coordinates was constructed. The analysis shows that an apparent difference in transcriptional responses to the conceptus exists between the endometrial LE from the two anatomically distinct regions in the uterus. In addition, we identified the communication pathways that link the conceptus and endometrial LE and found that these pathways have important roles in conceptus attachment. Furthermore, a number of genes whose expression is spatially restricted in the two different anatomical regions within the uterus were characterized for the first time and two of them (SULT2A1 and MEP1B) may cooperatively contribute to establish conceptus attachment in pigs. The results from our study have implications in understanding of conceptus/embryo attachment in pigs and other large polytocous species.

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Section: Resultsmentioning

confidence: 99%

Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs

Wang

Zhao

Deng

et al. 2021

IJMS

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“…In parallel, many factors were upregulated that promote cell differentiation and establishment of epithelial layers (Plet1) and limit inflammation, e.g. Tnip3 ( Abin3) inhibiting NF K B-induced TLR4 and IL-1 responses [ 41 , 42 ] ( Fig 5B and 5C , S1 – S3 Datasets).…”

Section: Resultsmentioning

confidence: 99%

Loss of CNFY toxin-induced inflammation drives Yersinia pseudotuberculosis into persistency

et al. 2018

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Gastrointestinal infections caused by enteric yersiniae can become persistent and complicated by relapsing enteritis and severe autoimmune disorders. To establish a persistent infection, the bacteria have to cope with hostile surroundings when they transmigrate through the intestinal epithelium and colonize underlying gut-associated lymphatic tissues. How the bacteria gain a foothold in the face of host immune responses is poorly understood. Here, we show that the CNFY toxin, which enhances translocation of the antiphagocytic Yop effectors, induces inflammatory responses. This results in extensive tissue destruction, alteration of the intestinal microbiota and bacterial clearance. Suppression of CNFY function, however, increases interferon-γ-mediated responses, comprising non-inflammatory antimicrobial activities and tolerogenesis. This process is accompanied by a preterm reprogramming of the pathogen's transcriptional response towards persistence, which gives the bacteria a fitness edge against host responses and facilitates establishment of a commensal-type life style.

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“…Since the role of posttranscriptional modification has gradually gained attention, more and more studies have also focused on the polyadenylation of single gene in pig [46][47][48]. Many methods based on NGS technology currently emerged as the times require, including poly(A) site sequencing…”

Section: Discussionmentioning

confidence: 99%

“…Finally, we took the simultaneous existence of genes that are associated with tissue-specific PASs above and the predicted differential target genes of miRNA and RBPs. Some shared genes were discovered to be involved in muscle fiber transformation, such as HDAC (47)(48)(49), TRIB3 (50), and GYS1 [45].…”

Section: Annotation Of Mirnas and Rbps To Detect Possiblementioning

confidence: 99%

Characterization and Functional Analysis of Polyadenylation Sites in Fast and Slow Muscles

Deng

Zou

et al. 2020

BioMed Research International

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Many increasing documents have proved that alternative polyadenylation (APA) events with different polyadenylation sites (PAS) contribute to posttranscriptional regulation. However, little is known about the detailed molecular features of PASs and its role in porcine fast and slow skeletal muscles through microRNAs (miRNAs) and RNA binding proteins (RBPs). In this study, we combined single-molecule real-time sequencing and Illumina RNA-seq datasets to comprehensively analyze polyadenylation in pigs. We identified a total of 10,334 PASs, of which 8734 were characterized by reference genome annotation. 32.86% of PASassociated genes were determined to have more than one PAS. Further analysis demonstrated that tissue-specific PASs between fast and slow muscles were enriched in skeletal muscle development pathways. In addition, we obtained 1407 target genes regulated by APA events through potential binding 69 miRNAs and 28 RBPs in variable 3′ UTR regions and some are involved in myofiber transformation. Furthermore, the de novo motif search confirmed that the most common usage of canonical motif AAUAAA and three types of PASs may be related to the strength of motifs. In summary, our results provide a useful annotation of PASs for pig transcriptome and suggest that APA may serve as a role in fast and slow muscle development under the regulation of miRNAs and RBPs.

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Cellular Localization and Regulation of Expression of the PLET1 Gene in Porcine Placenta

Cited by 8 publications

References 47 publications

Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs

Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs

Loss of CNFY toxin-induced inflammation drives Yersinia pseudotuberculosis into persistency

Characterization and Functional Analysis of Polyadenylation Sites in Fast and Slow Muscles

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