Cellular-level near-wall unsteadiness of high-hematocrit erythrocyte flow using confocal μPIV

Patrick, Michael J.; Chen, Chia Yuan; Dur, Onur; Pekkan, Kerem

doi:10.1007/s00348-010-0943-8

Cited by 34 publications

(30 citation statements)

References 53 publications

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“…The presented lPIV calculation algorithm was validated by comparing the experimental and theoretical data with high agreement, which can be found in the literature. 15,27 III. RESULTS…”

Section: Methodsmentioning

confidence: 99%

Microscale flow propulsion through bioinspired and magnetically actuated artificial cilia

et al. 2015

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Recent advances in microscale flow propulsion through bioinspired artificial cilia provide a promising alternative for lab-on-a-chip applications. However, the ability of actuating artificial cilia to achieve a time-dependent local flow control with high accuracy together with the elegance of full integration into the biocompatible microfluidic platforms remains remote. Driven by this motive, the current work has constructed a series of artificial cilia inside a microchannel to facilitate the timedependent flow propulsion through artificial cilia actuation with high-speed (>40 Hz) circular beating behavior. The generated flow was quantified using micro-particle image velocimetry and particle tracking with instantaneous net flow velocity of up to 10 1 lm/s. Induced flow patterns caused by the tilted conical motion of artificial cilia constitutes efficient fluid propulsion at microscale. This flow phenomenon was further measured and illustrated by examining the induced flow behavior across the depth of the microchannel to provide a global view of the underlying flow propulsion mechanism. The presented analytic paradigms and substantial flow evidence present novel insights into the area of flow manipulation at microscale. V C 2015 AIP Publishing LLC. [http://dx

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“…The presented lPIV calculation algorithm was validated by comparing the experimental and theoretical data with high agreement, which can be found in the literature. 15,27 III. RESULTS…”

Section: Methodsmentioning

confidence: 99%

Microscale flow propulsion through bioinspired and magnetically actuated artificial cilia

et al. 2015

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“…Four-thousand frames were recorded per data set, encompassing ~20 cardiac cycles. The pulsatile velocity distribution of red blood cell flow in the distal first aortic arch was calculated using timelapsed images following a previously validated particle image velocimetry (PIV) measurement protocol (Patrick et al, 2010). Briefly, a fast Fourier transform cyclic algorithm was used to process PIV time series images, and velocity vectors were calculated using multi-pass cross-correlation algorithms (DaVis 7.2 PIV software, LaVision, Ypsilanti, MI, USA).…”

Section: Confocal Imaging and Microangiographymentioning

confidence: 99%

“…To determine whether shear stress is altered in alk1 mutants, we used high-speed confocal micro-particle image velocimetry (Patrick et al, 2010) to generate averaged velocity profiles and calculate wall shear stress (WSS) in alk1 mutants and their wild-type siblings. We measured WSS in the distal first aortic arch, which expresses alk1 and, in alk1 mutants, enlarges and exhibits changes in shear-responsive gene expression (see Fig.…”

Section: Fig 2 Retention Of Normally Transient Arteriovenous Connecmentioning

confidence: 99%

Interaction between alk1 and blood flow in the development of arteriovenous malformations

et al. 2011

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SUMMARYArteriovenous malformations (AVMs) are fragile direct connections between arteries and veins that arise during times of active angiogenesis. To understand the etiology of AVMs and the role of blood flow in their development, we analyzed AVM development in zebrafish embryos harboring a mutation in activin receptor-like kinase I (alk1), which encodes a TGFb family type I receptor implicated in the human vascular disorder hereditary hemorrhagic telangiectasia type 2 (HHT2). Our analyses demonstrate that increases in arterial caliber, which stem in part from increased cell number and in part from decreased cell density, precede AVM development, and that AVMs represent enlargement and stabilization of normally transient arteriovenous connections. Whereas initial increases in endothelial cell number are independent of blood flow, later increases, as well as AVMs, are dependent on flow. Furthermore, we demonstrate that alk1 expression requires blood flow, and despite normal levels of shear stress, some flow-responsive genes are dysregulated in alk1 mutant arterial endothelial cells. Taken together, our results suggest that Alk1 plays a role in transducing hemodynamic forces into a biochemical signal required to limit nascent vessel caliber, and support a novel two-step model for HHT-associated AVM development in which pathological arterial enlargement and consequent altered blood flow precipitate a flow-dependent adaptive response involving retention of normally transient arteriovenous connections, thereby generating AVMs.

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“…Though the exact molecular mechanisms involved in the relationship between aortic arch hemodynamics, gene expression, and tissue remodeling are not yet fully understood, τ w is believed to be the primary mechanical stimulus for vascular remodeling (Culver and Dickinson 2010;Rodbard 1975), clinically referred to as the "flow-dependency principle" (Kamiya and Togawa 1980;Lu et al 2001;Rodbard 1975). The temporal and spatial events associated with flow sensitive altered aortic morphogenesis (at the first aortic arch) in the Akl-1 mutant zebrafish model can be more complex as illustrated by recent studies Corti et al 2011;Patrick et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Computational hemodynamic optimization predicts dominant aortic arch selection is driven by embryonic outflow tract orientation in the chick embryo

Kowalski

Teslovich

Dur

et al. 2012

Biomech Model Mechanobiol

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In the early embryo, a series of symmetric, paired vessels, the aortic arches, surround the foregut and distribute cardiac output to the growing embryo and fetus. During embryonic development the arch vessels undergo large-scale asymmetric morphogenesis to form species-specific adult great vessel patterns. These transformations occur within a dynamic biomechanical environment, which can play an important role in the development of normal arch configurations or the aberrant arch morphologies associated with congenital cardiac defects. Arrested migration and rotation of the embryonic outflow tract during late stages of cardiac looping has been shown to produce both outflow tract and several arch abnormalities. Here we investigate how changes in flow distribution

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Cellular-level near-wall unsteadiness of high-hematocrit erythrocyte flow using confocal μPIV

Cited by 34 publications

References 53 publications

Microscale flow propulsion through bioinspired and magnetically actuated artificial cilia

Microscale flow propulsion through bioinspired and magnetically actuated artificial cilia

Interaction between alk1 and blood flow in the development of arteriovenous malformations

Computational hemodynamic optimization predicts dominant aortic arch selection is driven by embryonic outflow tract orientation in the chick embryo

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