Cellular IRES-mediated translation

Komar, Anton A.; Hatzoglou, Maria

doi:10.4161/cc.10.2.14472

Cited by 349 publications

(252 citation statements)

References 116 publications

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“…IRES activity is regulated by many IRES-dependent translational regulators called ITAFs. 44 For example, UNR IRES activity is repressed by PTB and UNR itself and activated by hnRNP C1/ C2 in 293T cells. 22 Thus, an as yet unknown IRES-dependent firefly luciferase gene from pGL4.12 (Promega).…”

Section: Methodsmentioning

confidence: 99%

RETRACTED: Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11^p58 in mouse embryonic stem cells

Ohno

Shibayama²,

Sato³

et al. 2011

Cell Cycle

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Section: Methodsmentioning

confidence: 99%

RETRACTED: Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11^p58 in mouse embryonic stem cells

Ohno

Shibayama²,

Sato³

et al. 2011

Cell Cycle

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“…Some of these nuclear RNA-binding proteins are known as ITAFs. ITAFs are thought to recognize IRES and could contribute to translation of IRES-containing mRNAs [66][67][68]. Although the role of IRES in mitosis is controversial, it has been proposed that IRESs, through ITAFs, allow recruitment of translational machinery independently of eIF4E, which is largely inhibited in mitosis [45,46,[69][70][71][72].…”

Section: Translational Regulation Of Cell Cycle Transitions In Mitotimentioning

confidence: 99%

Translational regulation of the cell cycle: when, where, how and why?

Kronja

Orr‐Weaver

2011

Phil. Trans. R. Soc. B

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Translational regulation contributes to the control of archetypal and specialized cell cycles, such as the meiotic and early embryonic cycles. Late meiosis and early embryogenesis unfold in the absence of transcription, so they particularly rely on translational repression and activation of stored maternal mRNAs. Here, we present examples of cell cycle regulators that are translationally controlled during different cell cycle and developmental transitions in model organisms ranging from yeast to mouse. Our focus also is on the RNA-binding proteins that affect cell cycle progression by recognizing special features in untranslated regions of mRNAs. Recent research highlights the significance of the cytoplasmic polyadenylation element-binding protein (CPEB). CPEB determines polyadenylation status, and consequently translational efficiency, of its target mRNAs in both transcriptionally active somatic cells as well as in transcriptionally silent mature Xenopus oocytes and early embryos. We discuss the role of CPEB in mediating the translational timing and in some cases spindle-localized translation of critical regulators of Xenopus oogenesis and early embryogenesis. We conclude by outlining potential directions and approaches that may provide further insights into the translational control of the cell cycle.

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“…5C). This result is not unexpected if APP mainly regulates eIF‐4A functions, since this factor is also heavily involved in IRES‐dependent translation initiation (Komar and Hatzoglou, 2011). …”

Section: Resultsmentioning

confidence: 80%

Amyloid Precursor Protein (APP) Affects Global Protein Synthesis in Dividing Human Cells

Sobol¹,

Galluzzo²,

Liang³

et al. 2015

Journal Cellular Physiology

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Hypoxic non‐small cell lung cancer (NSCLC) is dependent on Notch‐1 signaling for survival. Targeting Notch‐1 by means of γ‐secretase inhibitors (GSI) proved effective in killing hypoxic NSCLC. Post‐mortem analysis of GSI‐treated, NSCLC‐burdened mice suggested enhanced phosphorylation of 4E‐BP1 at threonines 37/46 in hypoxic tumor tissues. In vitro dissection of this phenomenon revealed that Amyloid Precursor Protein (APP) inhibition was responsible for a non‐canonical 4E‐BP1 phosphorylation pattern rearrangement—a process, in part, mediated by APP regulation of the pseudophosphatase Styx. Upon APP depletion we observed modifications of eIF‐4F composition indicating increased recruitment of eIF‐4A to the mRNA cap. This phenomenon was supported by the observation that cells with depleted APP were partially resistant to silvestrol, an antibiotic that interferes with eIF‐4A assembly into eIF‐4F complexes. APP downregulation in dividing human cells increased the rate of global protein synthesis, both cap‐ and IRES‐dependent. Such an increase seemed independent of mTOR inhibition. After administration of Torin‐1, APP downregulation and Mechanistic Target of Rapamycin Complex 1 (mTORC‐1) inhibition affected 4E‐BP1 phosphorylation and global protein synthesis in opposite fashions. Additional investigations indicated that APP operates independently of mTORC‐1. Key phenomena described in this study were reversed by overexpression of the APP C‐terminal domain. The presented data suggest that APP may be a novel regulator of protein synthesis in dividing human cells, both cancerous and primary. Furthermore, APP appears to affect translation initiation using mechanisms seemingly dissimilar to mTORC‐1 regulation of cap‐dependent protein synthesis. J. Cell. Physiol. 230: 1064–1074, 2015. © 2014 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc.

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Cellular IRES-mediated translation

Cited by 349 publications

References 116 publications

RETRACTED: Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11^p58 in mouse embryonic stem cells

RETRACTED: Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11^p58 in mouse embryonic stem cells

Translational regulation of the cell cycle: when, where, how and why?

Amyloid Precursor Protein (APP) Affects Global Protein Synthesis in Dividing Human Cells

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Cellular IRES-mediated translation

Cited by 349 publications

References 116 publications

RETRACTED: Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11p58 in mouse embryonic stem cells

RETRACTED: Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11p58 in mouse embryonic stem cells

Translational regulation of the cell cycle: when, where, how and why?

Amyloid Precursor Protein (APP) Affects Global Protein Synthesis in Dividing Human Cells

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Product

Resources

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RETRACTED: Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11^p58 in mouse embryonic stem cells

RETRACTED: Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11^p58 in mouse embryonic stem cells