Cellular impedance sensing combined with LAPS as a new means for real-time monitoring cell growth and metabolism

Wu, Chengxiong; Zhou, Jie; Hu, Ning; Ha, Da; Miao, Xin; Wang, Ping

doi:10.1016/j.sna.2013.05.010

Cited by 20 publications

(9 citation statements)

References 30 publications

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“…Despite the numerous and very interesting integrated approaches, 18–20 the goal of having a convenient and possibly disposable multi-sensing platform for such an application is yet to be achieved. This is mainly due to the cost of the devices and the rather complicated approaches employed so far.…”

Section: Introductionmentioning

confidence: 99%

An organic neurophysiological tool for neuronal metabolic activity monitoring

et al. 2018

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Monitoring cell metabolism in vitro is considered a relevant methodology in several scientific fields ranging from fundamental biology research to neuro-toxicology. In the last 20 years, several in vitro neuro-pharmacological and neuro-toxicological approaches have been developed, with the intent of addressing the increasing demand for real-time, non-invasive in vitro systems capable of continuously and reliably monitoring cellular activity. In this paper, an Organic Charge Modulated Field Effect Transistor-based device is proposed as a promising tool for neuro-pharmacological applications, thanks to its ultra-high pH sensitivity and a simple fabrication technology. The preliminary characterization of this versatile organic device with primary neuronal cultures shows how these remarkable properties can be exploited for the realization of ultra-sensitive metabolic probes, which are both reference-less and low cost. These features, together with the already assessed capability of this sensor to also monitor the electrical activity of electrogenic cells, could provide important advances in the fabrication of multi-sensing lab-on-chip devices, thus opening up interesting perspectives in the neuro-pharmacological field.

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Section: Introductionmentioning

confidence: 99%

An organic neurophysiological tool for neuronal metabolic activity monitoring

et al. 2018

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“…35 While early cell culture monitoring devices 36 measured acidification only using LAPS, this sensor principle was employed in recent multiparameter systems as well. 37,38 ISFETs with a pHsensitive gate material are among the smallest possible pH sensors employed in cell culture. 39,40 Both LAPS and ISFET are limited to the availability of silicon as the substrate including CMOS circuits (see also the section on microfabrication and materials).…”

Section: Acidification Phmentioning

confidence: 99%

“…[109][110][111] Newer implementations of the LAPS microphysiometry principle exist. 37,38,112 With similar protocols, pH was also measured by means of ion-selective field effect transistors (ISFET) using CMOS technology on silicon chips. 78,79,113 ISFETs also allowed the determination of dissolved oxygen by local pH changes.…”

Section: Systems For 2d Cell Culturestatic Conditionsmentioning

confidence: 99%

Microsensor systems for cell metabolism – from 2D culture to organ-on-chip

et al. 2018

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Microsensor systems for cell metabolism are essential tools for investigation and standardization in cell culture. Electrochemical and optical read-out schemes dominate, which enable the marker-free, continuous, online recording of transient effects and deliver information beyond microscopy and end-point tests. There has been much progress in microfluidics and microsensors, but the translation of both into standard cell culture procedures is still limited. Within this critical review, we discuss different cell culture formats ranging from standard culture vessels to dedicated microfluidic platforms. Key aspects are the appropriate supply of cells, mass transport of metabolites to the sensors and generation of stimuli. Microfluidics enable the transition from static to dynamic conditions in culture and measurement. We illustrate the parameters oxygen (respiration), pH (acidification), glucose and lactate (energy metabolism) as well as short-lived reactive species (ROS/RNS) from the perspective of microsensor integration in 2D and 3D cell culture. We discuss different sensor principles and types, along with their limitations, microfabrication technologies and materials. The state-of-the-art of microsensor platforms for cell culture is discussed with respect to sensor performance, the number of parameters and timescale of application. That includes the advances from 2D culture to the increasingly important 3D approaches, with specific requirements for organotypic microtissues, spheroids and solid matrix cultures. We conclude on the current progress, potential, benefits and limitations of cell culture monitoring systems from monolayer culture to organ-on-chip systems.

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“…C 60 is a classical π‐conjugated carbon molecular [ 17 ] with high n‐type conduction. [ 18 ] Owing to its good photo‐response, [ 14b ] excellent electron transport capacity, [ 18 ] and facile fabrication process, C 60 has been widely used in a variety of organic optoelectronic devices, including organic light‐emitting diodes, [ 19 ] photovoltaic cells, [ 20 ] and thin film transistors. [ 21 ] In addition, due to the excellent redox properties, chemical stability and biocompatibility, C 60 has also been used as an electron‐mediator in voltammetric sensors.…”

Section: Introductionmentioning

confidence: 99%

Bipolar Light‐Addressable Potentiometric Sensor Based on Fullerene Photosensitive Layer

Jiao

Zhang

et al. 2021

Adv Materials Technologies

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Light‐addressable potentiometric sensor (LAPS) is highly attractive in many sectors, including human disease detection, drug screening, and environmental monitoring. Currently, classical LAPS chips adopt inorganic semiconductors, such as silicon, as the photosensitive layer. Organic semiconductors can provide the next generation of the photosensitive layer because of their high absorption efficiency, large structural variability, eco‐friendliness, and bendability. However, no promising organic materials have been reported so far for the construction of LAPS chips. Here, the authors demonstrate the potential of fullerene (C60), a π‐conjugated carbon molecule with high n‐type conduction, as a candidate material for the photosensitive layer of LAPS. They report on a peculiar bipolar photo‐response under both reverse and forward biased conditions for C60‐based LAPS. An oxygen‐trap‐induced space‐charge mechanism is proposed to explain the unique bipolar photo‐response phenomenon. The proposed C60‐based LAPS exhibits an excellent pH sensitivity of 150 mV/pH as well as a high spatial resolution of 3.8 µm. The authors anticipate that these results will spark interest in this field and enable the replacement of the inorganic‐semiconductor photosensitive layer by organic semiconductor materials for the construction of high‐performance LAPS.

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Cellular impedance sensing combined with LAPS as a new means for real-time monitoring cell growth and metabolism

Cited by 20 publications

References 30 publications

An organic neurophysiological tool for neuronal metabolic activity monitoring

An organic neurophysiological tool for neuronal metabolic activity monitoring

Microsensor systems for cell metabolism – from 2D culture to organ-on-chip

Bipolar Light‐Addressable Potentiometric Sensor Based on Fullerene Photosensitive Layer

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