Cellular immunogenicity of a multi-epitope peptide vaccine candidate based on hepatitis C virus NS5A, NS4B and core proteins in HHD-2 mice

Huang, Xiao‐Jun; Lu, Xuanyong; Lei, Yingfeng; Yang, Jing; Yao, Min; Lan, Hai-yun; Zhang, Jianmin; Zhansheng, Jia; Yin, Weidong; Zhang, Xu

doi:10.1016/j.jviromet.2013.01.003

Cited by 29 publications

(18 citation statements)

References 29 publications

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“…Huang и соавт. [13] для стимуляции CD4+ и CD8+ T-клеточного ответа поместили Th-эпитоп из core-белка ВГС (KFPGGGQIVGGVYLLPRRG-PRL) между двумя цитотоксическими эпитопами. Авторы показали эффективность этой конструкции в индукции клеточного иммунного ответа по сравнению с рекомбинантным белком без этого Th-эпитопа.…”

Section: Discussionunclassified

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Immunogenic Properties of Recombinant Mozaic Proteins Based on Antigens Ns4a and Ns4b of Hepatitis C Virus

Куприянов

Николаева

Zykova

et al. 2018

Problems of Virology

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The aim of the study was to investigate immunogenic properties of mosaic recombinant proteins constructed on the data of hepatitis C virus NS4A and NS4B antigens. Four mosaic recombinant proteins, containing the T and B epitopes of the NS4A and NS4B antigens, were created by genetic engineering methods in the E. coli system. To enhance the immune response they were linked in different variations to the nucleotide sequences of murine interleukin-2 (IL-2), the Neisseria meningiditis lipopeptide, and the T helper epitope of the core protein of hepatitis C virus. The immunogenic properties of these recombinant proteins were analyzed by immunoblotting, ELISA and ELISpot using sera from immunized mice and patients infected with hepatitis C virus. Recombinant proteins specifically reacted with the sera of immunized mice and infected patients in immunoblotting. According to the ELISA data, the predominant formation of antibodies to NS4B was observed when mice were immunized with the recombinant proteins containing both antigens. Analysis of gamma-interferon production by T-lymphocytes upon contact with activated dendritic cells showed in ELISpot that the maximum production of this cytokine was detected when adjuvant components were located at the N- and C-ends of the recombinant protein. The highest level of gamma-interferon production during stimulation with this drug was detected in lymphocytes from the bone marrow and lymph nodes. The recombinant protein containing the T and B epitopes of NS4A and NS4B, murine IL-2 and the lipopeptide Neisseria meningiditis had the greatest immunostimulate effect among the four constructions. This recombinant protein formed nanoparticles of 100-120 nm in size.

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Section: Discussionunclassified

“…Ранее в белке NS4В выявлены и локализованы В-клеточные, Т-киллерные и T-хелперные эпитопы [12][13][14].…”

Section: Original Researchunclassified

Immunogenic Properties of Recombinant Mozaic Proteins Based on Antigens Ns4a and Ns4b of Hepatitis C Virus

Куприянов

Николаева

Zykova

et al. 2018

Problems of Virology

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“…Mice (n=3/group) received 3 intravenous injections of 100 μL PBS or NGD-MNPs (5 mg Fe/kg/injection) every two weeks. Mice were euthanized 10 days after the last immunization 26 . T lymphocyte proliferation was determined by the MTT assay.…”

Section: Methodsmentioning

confidence: 99%

Multimodality Imaging of Angiogenesis in a Rabbit Atherosclerotic Model by GEBP11 Peptide Targeted Nanoparticles

Su¹,

Wang²,

Han³

et al. 2017

Theranostics

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Background and Aims: Angiogenesis is an important pathological process during progression of plaque formation, which can result in plaque hemorrhage and vulnerability. This study aims to explore non-invasive imaging of angiogenesis in atherosclerotic plaque through magnetic resonance imaging (MRI) and positron emission tomography (PET) by using GEBP11 peptide targeted magnetic iron oxide nanoparticles in a rabbit model of atherosclerosis.Methods: The dual-modality imaging probe was constructed by coupling 2, 3-dimercaptosuccinnic acid-coated paramagnetic nanoparticles (DMSA-MNPs) and the PET 68Ga chelator 1,4,7-triazacyclononane-N, N', N''-triacetic acid (NOTA) to GEBP11 peptide. The atherosclerosis model was induced in New Zealand white rabbits by abdominal aorta balloon de-endothelialization and atherogenic diet for 12 weeks. The plaque areas in abdominal artery were detected by ultrasound imaging and Oil Red O staining. Immunofluorescence staining and Prussian blue staining were applied respectively to investigate the affinity of GEBP11 peptide. MTT and flow cytometric analysis were performed to detect the effects of NGD-MNPs on cell proliferation, cell cycle and apoptosis in Human umbilical vein endothelial cells (HUVECs). In vivo MRI and PET imaging of atherosclerotic plaque were carried out at different time points after intravenous injection of nanoparticles.Results: The NGD-MNPs with hydrodynamic diameter of 130.8 nm ± 7.1 nm exhibited good imaging properties, high stability, low immunogenicity and little cytotoxicity. In vivo PET/MR imaging revealed that 68Ga-NGD-MNPs were successfully applied to visualize atherosclerotic plaque angiogenesis in the rabbit abdominal aorta. Prussian blue and CD31 immunohistochemical staining confirmed that NGD-MNPs were well co-localized within the blood vessels' plaques.Conclusion: 68Ga-NGD-MNPs might be a promising MR and PET dual imaging probe for visualizing the vulnerable plaques.

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“…Increase in INFγ secreting cells production is believed to be characteristic of CSF infection [ 42 , 59 ]. In recent time, multi epitope candidate vaccine against Hepatitis C has been developed [ 24 ] which includes non-structural protein having HLA-A2 epitope. The candidate vaccine contains epitopes from NS5A, NS4B and core protein of Hepatitis C virus.…”

Section: Peptide As Vaccinesmentioning

confidence: 99%

Multiple antigenic peptide (MAP): a synthetic peptide dendrimer for diagnostic, antiviral and vaccine strategies for emerging and re-emerging viral diseases

et al. 2013

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The peptide dendrimer provides novel strategies for various biological applications. Assembling of peptide in macromolecular structure is expected to give rational models as drugs, their delivery and diagnostic reagents. Improved understanding of virus structure and their molecular interactions with ligands have paved the way for treatment and control of emerging and re-emerging viral diseases. This review presents a brief account of a synthetic peptide dendrimer used for diagnostic, therapeutic and prophylactic applications. The designs comprise of multiple antigenic peptides which are being used as alternate synthetic antigens for different viruses.

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Cellular immunogenicity of a multi-epitope peptide vaccine candidate based on hepatitis C virus NS5A, NS4B and core proteins in HHD-2 mice

Cited by 29 publications

References 29 publications

Immunogenic Properties of Recombinant Mozaic Proteins Based on Antigens Ns4a and Ns4b of Hepatitis C Virus

Immunogenic Properties of Recombinant Mozaic Proteins Based on Antigens Ns4a and Ns4b of Hepatitis C Virus

Multimodality Imaging of Angiogenesis in a Rabbit Atherosclerotic Model by GEBP11 Peptide Targeted Nanoparticles

Multiple antigenic peptide (MAP): a synthetic peptide dendrimer for diagnostic, antiviral and vaccine strategies for emerging and re-emerging viral diseases

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