Cellular immunity in man: Correlation of leukocyte migration inhibition factor formation and delayed hypersensitivity

Curtis, John E.; Hersh, Evan M.

doi:10.1016/0008-8749(73)90092-0

Cited by 18 publications

(10 citation statements)

References 18 publications

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“…Thus, polymorphs were predominant in the 6-h PPD reactions, and this converted to a predominance of mononuclear cells in later windows. However, compared to day 0 and day 7 KLH windows, mononuclear cells were not predominant in PPD reactions at 24 (Fig. 9), which coincided with the progressively attenuated delayed reactions and increasing immediate reactions described above ( Fig.…”

Section: Resultsmentioning

confidence: 52%

“…No subject had any visible reaction 24, 48, or 72 h after the initial 20 ,g KLH injection of day 0 (Fig. la), and half showed slight 24 and 48-h reactions at the 200 ,ug KLH injection site of day 0 (Fig. lb).…”

Section: Resultsmentioning

confidence: 96%

“…It is highly unlikely that antibodies mediate the delayed accumulations of basophils we have noted in 7-day KLHinduced Jones-Mote reactions. Human "late" cutaneous hypersensitivity reactions, mediated by IgE antibodies (31), peak at 6-12 h, are nearly gone by 24 h, always follow strong wheal and flare responses, and should be hapten specific (36). Further study will be necessary to determine the histology, specificity, and mechanism underlying the complex dual cutaneous responses we have noted on repeat KLH skin testing.…”

Section: Discussionmentioning

confidence: 93%

“…We have immunized normal subjects with KLH (20)(21)(22)(23), and to evaluate the ability to express a delayed-type hypersensitivity reaction (24). The (6).…”

Section: Discussionmentioning

confidence: 99%

“…Earlier studies of the cellular composition of clini- 6,12,24,36, and 48 h. The cover slips were air-dried and Wright's-stained. As a control on possible variations in staining from day to day, a stored peripheral blood smear known to contain basophils was also stained.…”

Section: Introductionmentioning

confidence: 99%

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Basophils in tuberculin and "Jones-Mote" delayed reactions of humans.

Askenase¹,

Atwood²

1976

J. Clin. Invest.

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A B S T R A C T Jones-Mote reactions are delayed, erythematous, and mildly indurated cutaneous reactions originally described in humans sensitized by skin injection of heterologous proteins. Similar reactions in guinea pigs contain many basophils and are called cutaneous basophil hypersensitivity. In contrast, guinea pigs immunized with mycobacterial adjuvants have classical tuberculin-type delayed hypersensitivity reactions, which contain few basophils. This has led to a new classification of delayed responses, based largely on the presence or absence of basophils. We induced sensitization for JonesMote reactions in 20 normal humans by intradermal injections of keyhole limpet hemocyanin. Skin tests with KLH 1 wk later showed erythematous and indurated delayed reactions in all subjects. Rebuck skin windows showed specific accumulations of basophils with a delayed time-course in 18 of 20 subjects. In 12 normals sensitized with oxazolone-keyhole limpet hemocyanin conjugates, skin reactions and in vitro lymphocyte stimulation showed carrier and not hapten specificity, suggesting that cutaneous responses were probably mediated by T cells.A comparative stuidy of strongly positive PPD skin tests in patients with tuiberctulosis showed significant basophil accuimulations in five of nine subjects. Thus, basophils occuirred in human tuibercullin and Jones-Mote reactions and were not a distinguishing feature of Jones-Mote reactions. We suggest that the occurrence of basophils at delayed reactions is under complex regulation and that basophil accumulations are an aspect of delayed hypersensitivity, rather than an indication of a distinctive and separate response. Dr. Askenase is the recipient of Allergic DiseasesAcademic Award AI-70829, and Dr. Atwood was the recipient of a student fellowship from the Syntex Corporation.

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Section: Resultsmentioning

confidence: 52%

Section: Resultsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 93%

“…We have immunized normal subjects with KLH (20)(21)(22)(23), and to evaluate the ability to express a delayed-type hypersensitivity reaction (24). The (6).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Basophils in tuberculin and "Jones-Mote" delayed reactions of humans.

Askenase¹,

Atwood²

1976

J. Clin. Invest.

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Leukocyte migration inhibition induced by the combination of drug and a liver constituent in patients with drug-induced hepatitis

Morizane

1978

Gastroenterol Jpn

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The leukocyte migration inhibition test in agarose medium was performed in 23 cases of clinically diagnosed drug-induced hepatitis. When the test antigen was the combination of soluble phase of a liver homogenate fractionated by Sephadex G-100 which should have contained liver specific antigen and the offending drug the leukocyte migration was inhibited in 86% of cases. Whereas none of 12 cases of drug allergy without hepatic injury showed a positive result with the same combination of antigens. Other organ homogenate-muscle and kidney-never gave positive results when mixed with the offending drugs in cases of drug-induced hepatitis. It was concluded that in hypersensitivity type drug-induced hepatitis cell-mediated immunity might be established to the complex of liver specific antigen and the drug.

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