Cellular hydrogels based on pH-responsive chitosan-hydroxyapatite system

Abstract

The development of bioactive injectable system as cell carrier with minimal impact on viability of encapsulated cells represents a great challenge. In the present work, we propose a new pH-responsive chitosan-hydroxyapatite-based hydrogel with sodium bicarbonate (NaHCO) as the gelling agent. The in situ synthesis of hydroxyapatite phase has resulted in stable composite suspension and final homogeneous hydrogel. The application of sodium bicarbonate has allowed non-cytotoxic fast gelation of chitosan-hydroxyapatite within 4min, and without excess of sodium ions concentration. Rheological properties of crosslinked hydrogel have demonstrated possible behaviour as 'strong physical hydrogel'. The live dead staining has confirmed good viability and dispersion, as well as proliferation of encapsulated cells by the culture time. Presented preliminary results show good potential of chitosan-hydroxyapatite/NaHCO as a cell carrier, whose impact on the cell differentiation need to be confirmed by encapsulation of other cell phenotypes.

Hydrogels Based on Chitosan and Chitosan Derivatives for Biomedical Applications

“…Composite materials have physiochemical properties different from those ones of initially individual materials and depend on the type of the formed product. For instance, CS-magnetite composite has biodegradability, biocompatibility, mechanical resistance, and magnetic sensibility [84], and the CS-hydroxyapatite composite is pH-sensitive [85]. CS-iron (III) chloride composite membrane has excellent permeability for different fluids [86].…”

Fabricación de recubrimientos compuestos de Bioglass®/poli(ɛ-capro-lactona) obtenidos por co-deposición electroforética sobre acero inoxidable

“…En este contexto, CORRAL NUÑEZ et al 2016 [46], reportaron la formación de aglomerados semejantes a estructuras con morfología similar a la del coliflor que fueron obtenidos luego de que las partículas de biovidrio se sometieran a ensayos de bioactividad en SBF, presentando valores de relación calcio/fósforo muy cercanos a la estructura estequiométrica de HA. Además, ROGINA et al 2017 [47], también informaron la formación de aglomerados con formas similares, las cuales estaban compuestas por cristales de HA en forma de placas nanométricas distribuidas homogéneamente en toda la estructura. Estos resultados están en concordancia con los reportados por VICHERY et al 2016 [48], el cual reportó que un vidrio bioactivo, se disuelve gradualmente liberando iones que promueven el crecimiento de una capa de hidroxiapatita carbonatada en su superficie.…”

In-Situ Forming pH and Thermosensitive Injectable Hydrogels to Stimulate Angiogenesis: Potential Candidates for Fast Bone Regeneration Applications

“…Therefore, some researchers have exploited different neutralising agents as additive to β-GP in thermosensitive chitosan hydrogels, such as Mg GP to investigate cell adhesion and proliferation via in-vitro osteosarcoma cells [37] and Ca GP to investigate mineralisation to ensure calcium phosphate mineralisation [38]. Furthermore, di-sodium carbonate (Na 2 CO 3 ) [39], and sodium bi-carbonate (NaHCO 3 ) [40] compounds have been investigated as pH and temperature sensitive hydrogels.…”

“…In addition to swelling and sustainable drug release, these hydrogels were cytocompatible and improved cell proliferation with anti-inflammatory properties [48]. Recently, pH and thermosensitive chitosan/hydroxyapatite hydrogels neutralised by sodium bicarbonate was integrated by in-situ formed hydroxyapatite have contributed to increment in pH for neutralisation, and good cell viability and proliferation was obtained in encapsulated cells [40].…”