Cellular Heterogeneity Facilitates the Functional Differences Between Hair Follicle Dermal Sheath Cells and Dermal Papilla Cells: A New Classification System for Mesenchymal Cells within the Hair Follicle Niche

Gan, Yuyang; Wang, Hailin; Du, Lijuan; Li, Kaitao; Qu, Qian; Liu, Weiwen; Sun, Pingping; Fan, Zhexiang; Wang, Jin; Chen, Ruosi; Hu, Zhiqi; Miao, Yong

doi:10.1007/s12015-022-10411-2

Cited by 4 publications

(5 citation statements)

References 72 publications

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“…The results of the ow cytometric indicated that the DPCs we isolated were highly positive for DPCspeci c markers [35] (Fig. 1C).…”

Section: Resultsmentioning

confidence: 79%

Dermal papilla cell-derived exosomes promote hair follicle regeneration during wound healing by activating Wnt/β‐catenin signaling pathway

Shang,

Li,

Zhang

et al. 2024

Preprint

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Hair follicle (HF) regeneration after skin injury remains a major clinical challenge. Dermal papilla cell-derived exosomes (DPC-Exos) have great potential to induce HF neogenesis. However, the role and mechanism of DPC-Exos in HF regeneration during wound healing are still unclear. In this study, the effect of DPC-Exos on fibroblasts in wound healing was explored for the first time. We found that DPC-Exos could promote the proliferation and migration of fibroblasts, and more importantly, enhance the hair-inducing capacity of fibroblasts. The fibroblasts treated with DPC-Exos could induce HF neogenesis in nude mice when combined with neonatal mice epidermal cells. In addition, the results of in vivo experiments showed that DPC-Exos could accelerate wound re-epithelialization, and promote HF regeneration. The expression levels of Wnt pathway transcription factor β-catenin and Lef1 were elevated in fibroblasts and the dermis of skin wounds after DPC-Exos treatment. Taken together, this study proved that DPC-Exos could promote HF regeneration during wound healing by activating fibroblasts and the Wnt/β-catenin signaling pathway, suggesting that DPC-Exos might be a promising therapeutic strategy for skin wound regenerative healing.

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“…The results of the ow cytometric indicated that the DPCs we isolated were highly positive for DPCspeci c markers [35] (Fig. 1C).…”

Section: Resultsmentioning

confidence: 79%

Dermal papilla cell-derived exosomes promote hair follicle regeneration during wound healing by activating Wnt/β‐catenin signaling pathway

Shang,

Li,

Zhang

et al. 2024

Preprint

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“…Recent studies have shown that hypoxia can stimulate the proliferation of dermal papilla cells [23] , dental pulp stem cells [24] , neural stem cells [25] , and mesenchymal stem cells [26] , among others [27][28][29] . To some extent, dermal papilla cells can be considered a unique source of mesenchymal stem cells [30] . As shown through these other stem cell studies, hypoxia mainly promotes the proliferation of stem cells.…”

Section: Discussionmentioning

confidence: 99%

“…As this self-renewal process significantly enlarges stem cell pools, the proliferation-promoting effect of hypoxia could increase tissue density rather than tissue volume. Specifically, our results showed that hypoxia decreased the PAX6-and CHX10-positive RPCs ratio [22][23][24][25][26][27][28][29][30][31][32][33][34] . A previous study using the adherent differentiation method to generate RPCs showed that a low-oxygen environment could increase the percentage of RPCs co-expressing PAX6 and CHX10 [35] .…”

Section: Discussionmentioning

confidence: 99%

Influence of hypoxia on retinal progenitor and ganglion cells in human induced pluripotent stem cell-derived retinal organoids

Du,

Wang,

et al. 2023

Int J Ophthalmol

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AIM: To observe the effect of low oxygen concentration on the neural retina in human induced pluripotent stem cell (hiPSC)-derived retinal organoids (ROs). METHODS: The hiPSC and a three-dimensional culture method were used for the experiments. Generated embryoid bodies (EBs) were randomly and equally divided into hypoxic and normoxic groups. Photographs of the EBs were taken on days 38, 45, and 52, and the corresponding volume of EBs was calculated. Simultaneously, samples were collected at these three timepoints, followed by fixation, sectioning, and immunofluorescence. RESULTS: The proportion of Ki67-positive proliferating cells increased steadily on day 38; this proliferation-promoting effect tended to increase tissue density rather than tissue volume. On days 45 and 52, the two groups had relatively similar ratios of Ki67-positive cells. Further immunofluorescence analysis showed that the ratio of SOX2-positive cells significantly increased within the neural retina on day 52 (P<0.05). In contrast, the percentage of PAX6- and CHX10-positive cells significantly decreased following hypoxia treatment at all three timepoints (P<0.01), except for CHX10 at day 45 (P>0.05). Moreover, the proportion of PAX6-/TUJ1+ cells within the neural retinas increased considerably (P<0.01, <0.05, <0.05 respectively). CONCLUSION: Low oxygen promotes stemness and proliferation of neural retinas, suggesting that hypoxic conditions can enlarge the retinal progenitor cell pool in hiPSC-derived ROs.

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“…The stem cell properties of the DP cells are recognized as essential for the hair-inducing effect of these stem cells [ 9 ]. DP cells can differentiate into multiple cell types, including skin cells and adipocytes, and the decrease in their pluripotency causes the decline of hair-regenerating properties [ 10 ]. In addition, hair loss, or alopecia, can occur when DP stem cells become damaged, depleted, or inactive.…”

Section: Introductionmentioning

confidence: 99%

Potential Natural Products Regulation of Molecular Signaling Pathway in Dermal Papilla Stem Cells

et al. 2023

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Stem cells have demonstrated significant potential for tissue engineering and repair, anti-aging, and rejuvenation. Hair follicle stem cells can be found in the dermal papilla at the base of the follicle and the bulge region, and they have garnered increased attention because of their potential to regenerate hair as well as their application for tissue repair. In recent years, these cells have been shown to affect hair restoration and prevent hair loss. These stem cells are endowed with mesenchymal characteristics and exhibit self-renewal and can differentiate into diverse cell types. As research in this field continues, it is probable that insights regarding stem cell maintenance, as well as their self-renewal and differentiation abilities, will benefit the application of these cells. In addition, an in-depth discussion is required regarding the molecular basis of cellular signaling and the influence of nature-derived compounds in stimulating the stemness properties of dermal papilla stem cells. This review summarizes (i) the potential of the mesenchymal cells component of the hair follicle as a target for drug action; (ii) the molecular mechanism of dermal papilla stem cells for maintenance of their stem cell function; and (iii) the positive effects of the natural product compounds in stimulating stemness in dermal papilla stem cells. Together, these insights may help facilitate the development of novel effective hair loss prevention and treatment.

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Cellular Heterogeneity Facilitates the Functional Differences Between Hair Follicle Dermal Sheath Cells and Dermal Papilla Cells: A New Classification System for Mesenchymal Cells within the Hair Follicle Niche

Cited by 4 publications

References 72 publications

Dermal papilla cell-derived exosomes promote hair follicle regeneration during wound healing by activating Wnt/β‐catenin signaling pathway

Dermal papilla cell-derived exosomes promote hair follicle regeneration during wound healing by activating Wnt/β‐catenin signaling pathway

Influence of hypoxia on retinal progenitor and ganglion cells in human induced pluripotent stem cell-derived retinal organoids

Potential Natural Products Regulation of Molecular Signaling Pathway in Dermal Papilla Stem Cells

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