Cellular functions of 14-3-3ζ in apoptosis and cell adhesion emphasize its oncogenic character

Niemantsverdriet, Maarten; Wagner, Kay-Dietrich; Visser, M.J.T.; Backendorf, Claude

doi:10.1038/sj.onc.1210742

Cited by 87 publications

(81 citation statements)

References 30 publications

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“…Short hairpin RNA targeting 14-3-3ζ increased the sensitivity of lung cancer cells to anoikis and reduced their anchorageindependent growth (32). Transient blockade of 14-3-3ζ expression by siRNA in (lung and breast) cancer cells reduced tumor growth in vivo, whereas 14-3-3ζ overexpression in MCF10A cells led to anchorage-independent growth and inhibited stress-induced apoptosis (19,33). Expressions of cell adhesion proteins (E-cadherin, T-cadherin, and γ-catenin) were elevated in 14-3-3ζ-depleted cells, indicating that 14-3-3ζ might be inhibitory toward cell-cell adhesion (33).…”

Section: Discussionmentioning

confidence: 99%

Small Interfering RNA Targeting 14-3-3ζ Increases Efficacy of Chemotherapeutic Agents in Head and Neck Cancer Cells

Matta

DeSouza

Ralhan

et al. 2010

Molecular Cancer Therapeutics

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Patients diagnosed in advanced stages of head and neck squamous cell carcinoma often show limited response to chemotherapeutic agents. Recently, we reported the overexpression of 14-3-3ζ protein in head and neck premalignant and cancer tissues using liquid chromatography-tandem mass spectrometry with isotopic labeling and revealed its significance as a prognostic marker using immunohistochemical analysis. In this study, we determined the potential of 14-3-3ζ as a therapeutic target for head and neck cancer. Small interfering RNA (siRNA) targeting 14-3-3ζ was used to downregulate its expression in head and neck cancer cells in culture. Cell cycle analysis showed that head and neck cancer cells transfected with siRNA targeting 14-3-3ζ showed G 2 -M arrest. These siRNA transfectants also showed increased cell death on treatment with any one of the following chemotherapeutic agents: cisplatin, 5-fluorouracil, paclitaxel, or doxorubicin in comparison with the no transfection controls. Flow cytometric analysis using propidium iodide staining showed increased sub-G 0 fraction in siRNA-transfected cells treated with any of these chemotherapeutic agents, suggesting cell death; in addition, Annexin V staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay revealed increased apoptosis. Taken together, our results strongly showed that downregulation of 14-3-3ζ expression may serve to improve the sensitivity of head and neck cancer cells to chemotherapeutic agents. Mol Cancer Ther; 9(10); 2676-88. ©2010 AACR.

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Section: Discussionmentioning

confidence: 99%

Small Interfering RNA Targeting 14-3-3ζ Increases Efficacy of Chemotherapeutic Agents in Head and Neck Cancer Cells

Matta

DeSouza

Ralhan

et al. 2010

Molecular Cancer Therapeutics

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“…41 Moreover, 14-3-3zeta shows a constant upregulation of the transcripts in a cottontail rabbit papillomavirus squamous carcinoma model employing New Zealand 14-3-3zeta in cancer X Yang et al White rabbits. 42 A web-based meta-analysis (Oncomine) reveals 14-3-3zeta overexpression in various types of carcinomas, 41 suggesting its oncogene property. Based on these evidences, we suggest that 14-3-3zeta is a candidate proto-oncogene and deserves further investigation into its role in carcinogenesis.…”

Section: Oncogenementioning

confidence: 99%

“…Apparently, the zeta isoform restrains both cell adhesion and the cellular propensity for apoptosis, two activities that are also restrained during carcinogenesis. 41 Moreover, 14-3-3zeta shows a constant upregulation of the transcripts in a cottontail rabbit papillomavirus squamous carcinoma model employing New Zealand 14-3-3zeta in cancer X Yang et al White rabbits. 42 A web-based meta-analysis (Oncomine) reveals 14-3-3zeta overexpression in various types of carcinomas, 41 suggesting its oncogene property.…”

Section: Oncogenementioning

confidence: 99%

Targeting 14-3-3zeta in cancer therapy

et al. 2011

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“…The PGK1|YWHAZ combination performed best with Geomean values of 1.57 (control calves) and 1.00 (high Se calves) and 1.19 (combined groups), which is similar or better than any single gene ( Table 8). Whereas PGK1 is an enzyme that is involved in glycolysis, YWHAZ plays a role in signal transduction by binding to phosphoserine containing proteins and is involved in many vital cellular processes, including metabolism, protein trafficking, apoptosis and cell cycle regulation [29]. More reliable qPCR data normalization should be achieved using a combination of genes from different biological pathways, rather than a single gene.…”

Section: Stability Of Neutrophil Reference Genes Evaluated In This Studymentioning

confidence: 99%

Reference gene selection for quantitative PCR studies in bovine neutrophils

Vorachek¹,

Bobe²,

Hall³

2013

ABB

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Reference genes are essential for studying mRNA expression with quantitative PCR (qPCR). We investigated 11 candidate whole-blood neutrophil reference genes (ACTB, B2M, G6PD, GAPDH, GYPC, HPRT, PGK1, RPL19, SDHA, TFRC, and YWHAZ) for beef calves, both males and females, with or without selenium supplementation. Initial screening was based on gene expression level (<28 Cq cycles), variability (SD < 1.5 Cq cycles), excluded GYPC and TFRC from further analysis. Expression stability of the remaining genes was evaluated using four software programs: geNorm, NormFinder, BestKeeper, and the comparative delta Cq method. The neutrophil reference genes, YWHAZ, PGK1, and RPL19, consistently ranked among the top four most stable genes under these experimental conditions. The commonly used reference genes, ACTB and HPRT, were not reliable, underscoring the need to validate neutrophil reference genes under different experimental conditions. Multiple reference genes rather than a single gene may provide more robust and reliable results. The best pair of reference genes in whole-blood neutrophils from beef calves overall was PGK1|YWHAZ.

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Cellular functions of 14-3-3ζ in apoptosis and cell adhesion emphasize its oncogenic character

Cited by 87 publications

References 30 publications

Small Interfering RNA Targeting 14-3-3ζ Increases Efficacy of Chemotherapeutic Agents in Head and Neck Cancer Cells

Small Interfering RNA Targeting 14-3-3ζ Increases Efficacy of Chemotherapeutic Agents in Head and Neck Cancer Cells

Targeting 14-3-3zeta in cancer therapy

Reference gene selection for quantitative PCR studies in bovine neutrophils

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