Cellular Fatty Acids of Pathogenic
            <i>Neisseria</i>

Moss, C W; Kellogg, Douglas S.; Farshy, David C.; Lambert, M A; Thayer, James D.

doi:10.1128/jb.104.1.63-68.1970

Cited by 30 publications

(18 citation statements)

References 11 publications

(10 reference statements)

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“…The pathogenic Neisseria spp. contain large amounts of C16:0, C16:1, C12:0, 30HC12:0, and C14:0 (107). In contrast to Moss et al (107), who reported essentially identical patterns of CFAs in N. gonorrhoeae and N. meningitidis, Jantzen et al (66) presented a numerical analysis of data that suggests that minor quantitative differences may be sufficient to reliably distinguish N. gonorrhoeae and N.…”

Section: Identification To Genus or Speciesmentioning

confidence: 99%

Applications of cellular fatty acid analysis.

Welch

1991

Clinical Microbiology Reviews

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Section: Identification To Genus or Speciesmentioning

confidence: 99%

Applications of cellular fatty acid analysis.

Welch

1991

Clinical Microbiology Reviews

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“…Moss et al (24) have reported a definitive analysis of the fatty acid composition of N. meningitidis and N. gonorrhoeae. Subsequently, Lambert et al (18) described the fatty acid composition of a number of nonpathogenic Neisseria species.…”

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confidence: 99%

“…Cultivation of cells. GCBDS plates were inoculated by loop and incubated for 18 to 24 h in a candle jar at 35 C. Subinoculations were made from these plates to either solid medium (GCBDS plates) or liquid medium. The liquid media employed in this study were designated (i) LGCBDS (which consisted of: proteose peptone no.…”

mentioning

confidence: 99%

“…The mixture was shaken, the chloroform phase was drawn off, and the water phase was reextracted with an additional volume of chloroform. The chloroform phases were pooled, reduced by rotary evaporator to a viscuous oil, dried in vacuo over P205 for 24 h, and weighed. Samples of purified lipid were dissolved in small volumes of chloroform-methanol, 2:1 (vol/vol), and stored at 0 C. The percentage of lipid was determined by calculation from weights of lipid extracts dried in vacuo and accompanying cell samples dried at 80 C overnight in aluminum weighing dishes.…”

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confidence: 99%

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Lipids of Branhamella catarrhalis and Neisseria gonorrhoeae

Beebe¹,

Wlodkowski²

1976

J Bacteriol

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Three strains of Branhamella catarrhalis and three strains of Neisseria gonorrhoeae were analyzed with regard to their phospholipid and neutral lipid composition. B. catarrhalis (ATCC 23246) contained 5.12 ± 0.34% lipid, determined gravimetrically, compared to 8.56 ± 0.15% and 9.73 ± 0.06% for two strains ofN. gonorrhoeae. Cardiolipin, phosphatidylglycerol, and phosphatidylethanolamine were identified in extracts of both species. In addition, B. catarrhalis contained small amounts of phosphatidylcholine, and N. gonorrhoeae contained small amounts of lyso-phosphatidylethanolamine, which accumulated with autolysis accompanying late cell culture growth. The kinetics of change of relative amounts of phospholipids in both species were measured and found to differ substantially. Neutral lipid accounted for 30.4% of the total lipid of B. catarrhalis (ATCC 23246) and 7.6% of the total lipid ofN. gonorrhoeae NYH 002. Hydrocarbons, triglycerides, free fatty acids, coenzyme Q, diglycerides, and free hydroxy fatty acids were identified in the neutral lipid fraction of both species. The three strains of N. gonorrhoeae, sensitive, intermediate, and resistant to penicillin, exhibited no significant difference in the composition or metabolism of phospholipid. ' Present address: Clinical Microbiology Service, Columbia-Presbyterian Medical Center, New York, N.Y. 10032.nation of the lipid composition of N. gonorrhoeae. They did not detect PC, but they did find large amounts of lyso-Pe.We present here a definitive analysis of the phospholipid and neutral lipid composition of Branhamella catarrhalis and N. gonorrhoeae. The strains of N. gonorrhoeae employed exhibited varying levels of resistance to penicillin, and the existence of correlative elements of lipid composition to antibiotic resistance was also evaluated.(This work was reported in part previously [Bull. N.Y. Acad. Med. Abstr. 51:1148 and was presented at the Annual Meeting of the American Society for Microbiology, Chicago, Ill., 12 to 17 May 1974.) MATERIALS AND METHODSCultures. The strains of B. catarrhalis employed were ATCC strains 23246, 8176 and 8193; the latter two were generously supplied by R. Weaver of the Center for Disease Control, Atlanta, Ga. The strains of N. gonorrhoeae employed were a strain supplied by R. Weaver designated F19 and clinical isolates of the New York Hospital Microbiology Laboratory that were designated NYH 002 and NYH 006. After reception the strains were examined for colonial and microscopic morphology, Gram stain, oxidase test, and fermentation of appropriate sugars. Cultures were maintained for short duration by daily (N. gonorrhoeae) or biweekly transfer (B. catarrhalis) on GC medium base agar plates supplemented with 1% supplement B (Difco; GCBDS) incubated at 35 C 168 on August 5, 2020 by guest

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“…In recent years, we have used gas-liquid chromatography, mass spectrometry, and associated analytical techniques to study the chemical composition and metabolic activity of microorganisms as a basis for their identification and classification. Chemical data from cellular fatty acids, isoprenoid quinones, sphingolipids, and cell wall amino acids have provided valuable information for the recognition of genus, species, and unclassified groups of various bacteria (3)(4)(5)(6)(7). Over the years, we have determined the fatty acid composition of all organisms currently included in the family Neisseriaceae by Bovre (1) except Kingella species (6,7).…”

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Cellular fatty acid composition of Kingella species, Cardiobacterium hominis, and Eikenella corrodens

et al. 1988

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We determined the cellular fatty acid composition of reference strains and clinical isolates of each of the three Kingella species, Cardiobacterium hominis, and Eikenella corrodens by using capillary gas chromatography. Kingella denitrificans and Kingella kingae contained myristic (14:0) and palmitic (16:0) acids as major acids, whereas cis-vaccenic (18:1 w7c) and palmitic acids were the major acids in Kingella indologenes, C. hominis, and E. corrodens. C. hominis differed from the other four species by the absence of 3-hydroxylauric (3-OH-12: 0) acid, from K. indologenes by the presence of 3-hydroxypalmitic (3-OH-16:0) acid, and from E. corrodens by the presence of 3-hydroxymyristic (3-OH-14:0) acid. E. corrodens contained a small amount (2%) of myristic acid, while the other four species contained moderate to large amounts (11 to 31%) of this acid.

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Cellular Fatty Acids of Pathogenic Neisseria

Cited by 30 publications

References 11 publications

Applications of cellular fatty acid analysis.

Applications of cellular fatty acid analysis.

Lipids of Branhamella catarrhalis and Neisseria gonorrhoeae

Cellular fatty acid composition of Kingella species, Cardiobacterium hominis, and Eikenella corrodens

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