Cellular fatty acids as chemical markers for differentiation of Yersinia pestis and Yersinia pseudotuberculosis

Tan, Y. H.; M, Wu; Liu, H.; Dong, Xing-qi; Guo, Zhaobiao; Song, Zhiwei; Li, Yan; Cui, Yu‐Jun; Song, Yajun; Du, Zongmin; Yang, Ruifu

doi:10.1111/j.1472-765x.2009.02762.x

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…The major fatty acids were analyzed using an HP6890 gas chromatograph (ver. A 5.01), as previously described (Tan et al, 2010). Supernatant from late-logarithmic phase ZY-312 and ATCC 25285 cultures was collected by centrifugation for 10 min at 3000 × g , and then analyzed by gas chromatography-mass spectrometry.…”

Section: Methodsmentioning

confidence: 99%

Safety Evaluation of a Novel Strain of Bacteroides fragilis

Wang¹,

Deng²,

et al. 2017

Front. Microbiol.

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Commensal non-toxigenic Bacteroides fragilis confers powerful health benefits to the host, and has recently been identified as a promising probiotic candidate. We previously isolated B. fragilis strain ZY-312 and identified it as a novel strain based on 16S rRNA sequencing and morphological analyses. We also determined that ZY-312 displayed desirable probiotic properties, including tolerance to simulated digestive fluid, adherence, and in vitro safety. In this study, we aim to investigate whether ZY-312 meets the safety criteria required for probiotic bacteria through comprehensive and systematic evaluation. Consequently, the fatty acid profile, metabolite production, and biochemical activity of strain ZY-312 were found to closely resemble descriptions of B. fragilis in Bergey’s manual. Taxonomic identification of strain ZY-312 based on whole genome sequencing indicated that ZY-312 and ATCC 25285 showed 99.99% similarity. The 33 putative virulence-associated factors identified in ZY-312 mainly encoded structural proteins and proteins with physiological activity, while the lack of bft indicated that ZY-312 was non-toxigenic. In vivo safety was proven in both normal and immune-deficient mice. The 11 identified antibiotic resistance genes were located on the chromosome rather than on a plasmid, ruling out the risk of plasmid-mediated transfer of antibiotic resistance. In vitro, ZY-312 showed resistance to cefepime, kanamycin, and streptomycin. Finally, and notably, ZY-312 exhibited high genetic stability after 100 passages in vitro. This study supplements the foundation work on the safety evaluation of ZY-312, and contributes to the development of the first probiotic representative from the dominant Bacteroidetes phylum.

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Section: Methodsmentioning

confidence: 99%

Safety Evaluation of a Novel Strain of Bacteroides fragilis

Wang¹,

Deng²,

et al. 2017

Front. Microbiol.

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“…As far as we are aware, all published fatty acid compositions of members of the genus Yersinia show percentages of cyclic C 17 : 0 higher than 10 % (Jantzen & Lassen, 1980;Whittaker et al, 2005;Tan et al, 2010;Nagarajan et al, 2005). The compositions of the isolate and B. aquatica DSM 5075…”

mentioning

confidence: 92%

“…The high proportion of cyclic heptadecanoic acid in the cells of strain D9 T and Y. frederiksenii DSM 18490 T may reflect the fact that the standard MIDI growth medium was suboptimal for these organisms. As far as we are aware, all published fatty acid compositions of members of the genus Yersinia show percentages of cyclic C 17 : 0 higher than 10 % (Jantzen & Lassen, 1980; Whittaker et al , 2005; Tan et al , 2010; Nagarajan et al , 2005). The compositions of the isolate and B. aquatica DSM 5075 T support the grouping of the isolate in the family Enterobacteriaceae and suggest that the considerable amount of C 14 : 0 and the lack of C 17 : 0 may be indicative for the membership of the genus Budvicia .…”

mentioning

confidence: 94%

Budvicia diplopodorum sp. nov. and emended description of the genus Budvicia

Lang

Schümann

Knapp

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, rod-shaped, weakly motile, non-spore-forming bacterium (D9 T ) was isolated from the gut of Cylindroiulus fulviceps (Diplopoda) on 1/3-strength nutrient agar plates. On the basis of 16S rRNA gene sequence similarity, strain D9T was shown to be phylogenetically closely related to the type strain of Budvicia aquatica, the sole species of the genus Budvicia, family Enterobacteriaceae.

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“…The ACQUITY UPLC System (Waters) was used in this study. Whole-cell fatty acid methyl esters were obtained by saponification, methylation and extraction followed by analysis using a standardized Microbial Identification System (Microbial ID) (Tan et al , 2010). The Sherlock standard library MOORE5 5.00 made it possible to standardize the bacterial fatty acid analysis (http://www.midi-inc.com) in .…”

mentioning

confidence: 99%

Bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces

Nakano

Onoda

Toh

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Strains HM2-1 and HM2-2 T were isolated from the faeces of a healthy infant and were characterized by determining their phenotypic and biochemical features and phylogenetic positions based on partial 16S rRNA gene sequence analysis. They were Gram-positive, obligately anaerobic, non-spore-forming, non-gas-producing, and catalase-negative non-motile rods. They did not grow at 15 or 45 6C in anaerobic bacterial culture medium, and their DNA G+C content was in the range 56-59 mol%. In enzyme activity tests, strains HM2-1 and HM2-2 T were positive for a/b-galactosidases and a/b-glucosidases but negative for b-glucuronidase and cystine arylamidase. An analysis of the cell-wall composition of strains HM2-1 and HM2-2 T revealed the presence of glutamic acid, alanine and lysine. The presence of fructose-6-phosphate phosphoketolase shows that isolates HM2-1 and HM2-2 T are members of the genus Bifidobacterium. These two isolates belong to the same species of the genus Bifidobacterium.

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Cellular fatty acids as chemical markers for differentiation of Yersinia pestis and Yersinia pseudotuberculosis

Cited by 10 publications

References 22 publications

Safety Evaluation of a Novel Strain of Bacteroides fragilis

Safety Evaluation of a Novel Strain of Bacteroides fragilis

Budvicia diplopodorum sp. nov. and emended description of the genus Budvicia

Bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces

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