Cellular Fatty Acid Composition in Pseudomonas Species

Ikemoto, Shigeaki; Kuraishi, Hiroshi; Komagata, Kazuo; Azuma, Ryozo; Suto, Tsuneji; Murooka, Haruyoshi

doi:10.2323/jgam.24.199

Cited by 88 publications

(53 citation statements)

References 7 publications

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“…Cellular fatty acids were methylated with 5% HCl-methanol (Ikemoto et al, 1978;Komagata and Suzuki, 1987). The methylated esters were analyzed by gas chromatography by using a model GC-9A chromatograph (Shimadzu Corp., Kyoto, Japan) equipped with a capillary column DB-23 (0.25 mm by 60 m; Agilent Technologies Inc., Palo Alto, NY, USA) and a hydrogen flame ionization detector.…”

Section: Methodsmentioning

confidence: 99%

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Recharacterization of Pseudomonas fulva Iizuka and Komagata 1963, and proposals of Pseudomonas parafulva sp. nov. and Pseudomonas cremoricolorata sp. nov.

Uchino

Sato²,

Uchimura

et al. 2001

J. Gen. Appl. Microbiol.

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Section: Methodsmentioning

confidence: 99%

“…fulva was reported to have C 16 : 0 , C 16 : 1 , and C 18 : 1 as the major cellular fatty acids (Ikemoto et al, 1978;Oyaizu and Komagata, 1983), and Q-9 as the major quinone (Oyaizu and Komagata, 1983;Yamada et al, 1982). On the basis of the 16S rRNA sequence, P. fulva belonged to the genus Pseudomonas sensu stricto (Anzai et al, 2000).…”

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confidence: 99%

Recharacterization of Pseudomonas fulva Iizuka and Komagata 1963, and proposals of Pseudomonas parafulva sp. nov. and Pseudomonas cremoricolorata sp. nov.

Uchino

Sato²,

Uchimura

et al. 2001

J. Gen. Appl. Microbiol.

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“…(Swings et al [28] transferred P . maltophilia to the genus Xanthomonas as Xanthomonas maltophilia, but, like other authors [ 5 , 14,15,21,22, 321, we use the name P. maltophilia in this paper.) Identification methods.…”

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confidence: 99%

“…HC1-methanol (14). Hydroxy acids were separated by thinlayer chromatography by the method of Oyaizu and Komagata (22).…”

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confidence: 99%

Two New Species of Pseudomonas: P. oryzihabitans Isolated from Rice Paddy and Clinical Specimens and P. luteola Isolated from Clinical Specimens

Kodama

Kimura

Komagata³

1985

International Journal of Systematic Bacteriology

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Two new Pseudomonas species which were isolated from rice paddy and clinical specimens (groups Ve-2 and Ve-1) are described. Strains of Pseudomonas oryzihabitans sp. nov. are yellow-pigmented, oxidase-negative, nonsporeforming, gram-negative, polarly monotrichously flagellated, rod-shaped organisms with deoxyribonucleic acid base compositions ranging from 63.9 to 65.6 mol% guanine plus cytosine, ubiquinone Q-9, major cellular fatty acids consisting of In the course of a study of the microflora of rice paddies, a large number of yellow-pigmented, gram-negative bacteria were isolated. Some of these organisms were identified as "Pseudomonas lacunogenes," which was studied by Goresline (7), and some characteristics of our isolates have been published previously (12). When we published the characteristics of our strains of "P. lacunogenes," the original strains which Goresline used for the description of this species and reference strains were not available from any culture collection or other sources. They are still unavailable. The strains of "P. lacunogenes" were a major component of the bacterial flora of normal rice paddies (11). However, this name is not on the Approved Lists of Bacterial Names (26). Recently, we isolated new strains similar to "P. lacunogenes" from normal rice paddies. These cultures were oxidase negative and resembled cultures that Weaver et al. (31), Tatum et al. (30), and Gilardi et al. (6) recognized as groups Ve-1 and Ve-2 of yellow-pigmented, oxidative, oxidase-negative, gram-negative, polarly flagellated, rodshaped, bacteria. Therefore, we attempted to identify our isolates from normal rice paddies by using authentic strains representative of groups Ve-1 and Ve-2.In this paper we describe two new species, Pseudomonas oryzihabitans sp. nov., which was isolated from normal rice paddies and clinical specimens (group Ve-2), and Pseudomonus luteola sp. nov., which was isolated from clinical specimens (group Ve-1). Chemical Research, Wako-shi, Saitama, Japan; NCIB, National Collection of Industrial Bacteria, Torrey Research Station, Aberdeen, United Kingdom; DNA, deoxyribonucleic acid; G+C, guanine plus cytosine; DNase, deoxyribonuclease; ONPG, o-nitrophenyl-P-galactopyranoside. The designations used for ubiquinones indicate the number of isoprene units in a side chain (e.g., ubiquinones Q-8, Q-9, and Q-10, have 8, 9, and 10 isoprene units, respectively). MATERIALS AND METHODSBacterial strains. The strains which we studied are indicated below (names which do not appear on the Approved Lists of Bacterial Names [26] are enclosed in quotation marks)., and KS0904 (= D-2 = AJ 2210) were isolated from normal rice paddies by Iizuka and Komagata (12). Strain KS0905 was isolated from Oryza sativa strain C5444 (grain harvested in 1979), strain KS0906 was isolated from 0. sativa strain C8895 (grain harvested in 1979), strain KS0907 was isolated from 0. sativa strain (28896 (grain harvested in 1979), strain KS0908 was isolated from Oryza breviligulata strain W1064 (grain harvested in 1977), strain KS09...

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“…The analysis of the fatty acids was performed according to the method of IKEMOTO et al (1978). Twenty milligrams of lyophilized cells were methylated with 5% HC1-methanol at lOOT for 3 h. Extracted and concentrated fatty acids were analyzed by using a Shimadzu Model 9 A gas chromatograph with a glass column packed with Chromosorb W (60-80 mesh) coated with \0% djethylene glycol succinate (DEGS).…”

Section: Methodsmentioning

confidence: 99%