Cellular distribution of estrogen receptor β in neonatal rat bone

Windahl, Sara H; Norgård, Maria; Kuiper, George G. J. M.; Gustafsson, Jan‐Åke; Andersson, Göran

doi:10.1016/s8756-3282(99)00248-3

Cited by 59 publications

(39 citation statements)

References 24 publications

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“…This is in agreement with some, but in disagreement with the results of others (10 -14, 37, 38). Most ER studies were performed in osteosarcoma cell lines, which are dedifferentiated into an embryonic state (13,14,36,37) or in bones of immature rats or individuals with healing fractures or various bone diseases (11). Indeed, in developing bones our quantitative real-time RT-PCR system picks up ERb mRNA readily (authors' unpublished data).…”

Section: Discussionmentioning

confidence: 99%

“…Both receptor subtypes have been demonstrated in the bones of immature rats and osteoblast cell lines (10)(11)(12)(13). Whether the bones of adult rats express both or only the ERa subtype is controversial (14,15).…”

Section: Introductionmentioning

confidence: 99%

“…Many of these effects are mediated by a stimulation of osteoblast IGF-I production (21,22). The question as to whether or not osteoclasts are estrogen receptive and, if so, which ER type is being expressed, is much less clear (11,14,15). The effects of estrogens on tartrate-resistant acid phosphatase (TRAP), an osteoclast product, have been described which, however, could be indirectly exerted via paracrine mechanisms, through factors released by osteoblasts.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for selective estrogen receptor modulator activity in a black cohosh (Cimicifuga racemosa) extract: comparison with estradiol-17beta

Seidlová-Wuttke

Hesse²,

Jarry³

et al. 2003

European Journal of Endocrinology

125

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Objective: Some phytoestrogens are believed to have selective estrogen receptor modulator (SERM) activity with no action in the uterus but beneficial effects in the hypothalamo/pituitary unit and in the bone and are presently the focus of clinical interest. In the present experiments, the effects of the clinically used Cimicifuga racemosa (CR) extract BNO 1055 in the uterus, in the bone and on serum luteinizing hormone (LH) were compared with the effects of estradiol-17b (E 2 ) under acute and chronic conditions in ovariectomized rats. Methods: Ovariectomized rats were treated either acutely (6 h) or chronically (3 months) with E 2 or the CR extract. Gene expression of some estrogen-regulated genes in the metaphysis of the tibia and the uterus was determined. Furthermore, bone mineral density was measured by quantitative computer tomography. Results: When given acutely, both E 2 and the CR extract inhibited LH secretion and slightly stimulated gene expression of IGF-I, collagen-1a1, osteoprotegerin and osteocalcin (all osteoblast products), and of tartrate-resistant acid phosphatase (TRAP, an osteoclast product) in the metaphysis of the femur. While E 2 stimulated uterine weight and expression of progesterone receptor (PR), the complement protein (C3) and IGF-I genes, and inhibited gene expression of the estrogen receptor b (ERb) in the uterus, no such effect was observed under acute CR treatment. After chronic application with pelleted food over 3 months E 2 had profound effects in the uterus on weight and gene expression (ERb, PR, C3 and IGF-I) which were not seen in the CR-treated animals. Within 3 months after ovariectomy, control rats had lost more than 50% of the metaphyseal bone mass of the tibia, an effect prevented by E 2 and partially by CR supplementation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evidence for selective estrogen receptor modulator activity in a black cohosh (Cimicifuga racemosa) extract: comparison with estradiol-17beta

Seidlová-Wuttke

Hesse²,

Jarry³

et al. 2003

European Journal of Endocrinology

125

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“…Still, androgens seem to fulfill an essential role in establishing sexual differences in the skeleton, in both rats and humans (Turner et al 1994b, Dagogo-Jack et al 1997, Vanderschueren et al 1998. Evidence is gathering that androgens and estrogens can exert direct effects on bone cells, since both ERs (ER and ER ) and androgen receptor (AR) mRNA and protein have been demonstrated in osteoblasts, lining cells, osteocytes and osteoclasts in several species, including the rat, rabbit and human (Braidman et al 1995, Kusec et al 1998, Noble et al 1999, Vidal et al 1999, Windahl et al 2000, van der Eerden et al 2002b. However, to date ER and ER expression in the metaphysis of rat tibiae before and during sexual maturation has not been extensively studied.…”

Section: Introductionmentioning

confidence: 99%

Expression of estrogen receptors and enzymes involved in sex steroid metabolism in the rat tibia during sexual maturation

Eerden¹,

Löwik²,

Wit³

et al. 2004

Journal of Endocrinology

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Estrogens are essential for bone mass accrual but their role before sexual maturation has remained elusive. Using in situ hybridization and immunohistochemistry, we investigated the expression of both estrogen receptor (ER) and mRNA and protein as well as several mRNAs coding for enzymes involved in sex steroid metabolism (aromatase, type I and II 17 -hydroxysteroid dehydrogenase (17 -HSD), steroid sulfatase (STS) and type I 5 -reductase) on sections of tibial metaphyses before (1-and 4-week-old), during (7-week-old) and after (16-weekold) sexual maturation in female and male rats. ER and ER mRNA and protein were detected in metaphyseal bone in lining cells, osteoblasts, osteoclasts and some osteocytes with no apparent differences in expression during development or between the sexes. In contrast, aromatase, type I and II 17 -HSD and type I 5 -reductase mRNAs were first detected in osteoblasts, osteoclasts and occasionally in osteocytes from sexual maturation (7-week-old rat) and onwards. Only STS was present before sexual maturation. To study the significance of ER and expression in bone before sexual maturation when circulating sex steroid levels are low, 26-day-old female and male rats underwent gonadectomy or 17 -estradiol (E 2 ) supplementation (0·5 mg/21 days) during 3 weeks. Following gonadectomy, trabecular bone volume (TBV) was lower in males (P=0·03) and there was a trend towards reduction in females (P=0·057). E 2 supplementation increased tibial TBV compared with controls in both genders as assessed by Masson-Goldner staining. These data suggest that the presence of ERs in bone cells before sex maturation might be of significance for bone mass accrual. Furthermore, based on the mRNA expression of the crucial enzymes aromatase and type I 17 -HSD, we suggest that bone cells in the tibial metaphysis acquire the intrinsic capacity to metabolize sex steroids from sexual maturation onwards. This process may contribute to the beneficial effects of estrogen on bone mass accrual, possibly by intracrinology.

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“…Next, we tested the involvement of estrogen receptors (ERα and ERβ) [20][21][22] in estradiol effects on HPSCs. Despite their well-established expression in bone, mRNA of both receptors is expressed also in HPSCs at comparable levels to that in ovaries, expressing high levels of ERα and ERβ (Online Supplementary Figure S2A and B).…”

Section: Estrogen Receptors Erα and Erβ Are Redundant For The Effectsmentioning

confidence: 99%

Estradiol increases hematopoietic stem and progenitor cells independent of its actions on bone

Illing¹,

Liu²,

Ostermay³

et al. 2012

Haematologica

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Animal experiments were performed according to accepted standards of animal welfare and with the permission of the authorities of Thüringen. Estrogen treatment was given for four weeks by subcutaneous implantation of slow-release pellets resulting in a calculated dose of 0.24 mg/kg/d (0.36 mg; 60-day release; Innovative Research of America, Inc.) in 10-12 week old female C57BL/6 or CD45.1 (wild-type) mice and in ERα-and ERβ-knockout, ERα−loxP Runx2Cre mice. 7-10 Mice received short-term treatment with estradiol 5 mg/kg (Sigma) i.p. daily. Bone sections and von Kossa stainingLumbar vertebral bodies (L3-L5) and one tibia of each mouse were processed and stained, and bone histomorphometry was performed, all as previously described. 9Estradiol increases hematopoietic stem and progenitor cells independent of its actions on bone August 5, 2011. Revised version arrived on January 25, 2012. Manuscript accepted on February 13, 2012 Fax: international +49.731.5022581. Hematopoietic stem and progenitor cells reside in vascular and endosteal niches in the bone marrow. Factors affecting bone remodeling were reported to influence numbers and mobilization of hematopoietic stem cells. We therefore analyzed the effects of estradiol acting anabolic on bone integrity. Here we observe that estradiol increases progenitor cell numbers in the vascular but not in the endosteal compartment independent of its estrogen receptor α-dependent anabolic bone effects. Hematopoietic progenitors capable of reconstituting lethally irradiated mice are increased by enhanced cell cycle entry, leading to a diminished long-term reconstitution potential after serial transplantation. We demonstrate that estradiol action on stromal cells potently favors hematopoietic progenitor/stem cell frequency accompanied by enhanced expression of cell adhesion molecules. Finally, estradiol treatment enhances retention of hematopoietic stem cells in the vascular niche of the bone marrow. We describe for the first time the mechanism of estrogen action on hematopoietic stem and progenitor cells. Haematologica 2012;97(8): 1131-1135. doi:10.3324/haematol.2011 This is an open-access paper. ABSTRACT © F e r r a t a S t o r t i F o u n d a t i o n Isolation of hematopoietic cells of the vascular and endosteal nicheThe flushed fraction of the BM from hindlimbs and humeri represents the cells from the vascular niche. Endosteal BM cells were isolated as previously described. 11 The digested fraction represents the cells of the endosteal niche. Flow cytometryFlow cytometry was performed as described.12 Monoclonal antibodies from Natutec /eBioscience were:Gr1-FITC, B220-FITC, CD3-FITC, CD11b-FITC, Ter119-FITC, Sca1-PE,CD117-APC, CD150-PE, CD150-APC, CD48-PE, CD48-APC, CD45.1-FITC, B220-PE, Gr1-PE, CD11b-APC, CD4-PE, CD8-APC.Data were recorded with FACS-Canto II or FACS-Calibur (BDBiosciences) and analyzed by Flow Jo 8.0 flow cytometry software (Tristar). CAFC assayCobblestone area forming cell (CAFC) assay was performed as described 13 and frequency of HSC was calculated us...

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Cellular distribution of estrogen receptor β in neonatal rat bone

Cited by 59 publications

References 24 publications

Evidence for selective estrogen receptor modulator activity in a black cohosh (Cimicifuga racemosa) extract: comparison with estradiol-17beta

Evidence for selective estrogen receptor modulator activity in a black cohosh (Cimicifuga racemosa) extract: comparison with estradiol-17beta

Expression of estrogen receptors and enzymes involved in sex steroid metabolism in the rat tibia during sexual maturation

Estradiol increases hematopoietic stem and progenitor cells independent of its actions on bone

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