Cellular Differentiation, Ageing and Ion Transport

Steveninck, R. F. M. Van

doi:10.1007/978-3-642-66230-0_8

Cited by 14 publications

(8 citation statements)

References 211 publications

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“…In all organs -but best seen in organs with a determinate growth pattern -roID expression is quite high throughout the maturation period and in mature organs, but decreases in aged tissues and is eventually switched off with the first macroscopic signs of senescence. Ageing comprises a phase of adaptative ageing -maturation, when cells retain growth potential -and a phase of senescent ageing (true senescence) (van Steveninck 1976 (Capone et al 1991(Capone et al , 1994. It is also likely that the rolD promoter comprises different regulatory domains responsible for gene expression in specific tissues (phloem) and in specific developmental phases (elongation/maturation), through interaction with plant transacting factors.…”

Section: Discussionmentioning

confidence: 99%

TherolD gene fromAgrobacterium rhizogenes is developmentally regulated in transgenic tobacco

et al. 1997

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Summary. The rolD gene from Agrobacterium rhizogenes hasrecently been shown to induce striking precocity of flowering in transgenic tobacco. A transcriptional fusion between 578 bp of the rotD upstream regulating sequence and the GUS reporter gene has been transfelTed to tobacco plants and its expression analysed throughout the whole life cycle of the plant. A detailed histological analysis revealed that roll) is strongly expressed in elongating and differentiating tissues of each organ, from the mature embryo to the adult plant. In organs with determinate growth, such as cotyledons, leaves and floral parts, rolD expression follows the age gradient of the tissues. In mature tissues, tolD expression is high, begins to decrease with ageing and is switched off when senescence occurs.Roll) is also active in the vascular system, in the procambium, pithand receptacular-meristems. Our data show that expression of rolD is under developmental control as it correlates with, and may represent a molecular marker for, the elongation/expansion and maturation phases of the plant tissues. Keywords: Agrobacterium rhizogenes; rolD promoter; GUS gene;Nicotiana tabacum; Development.Abbreviations: PCR polymerase chain reaction; GUS 13-glucuronidase; X-Gluc 5-bromo-4-chloro-3-indolyl-[~-D-glucuronide; SE standard error; NT transcriptional terminator of the Nopaline synthase gene; NOS-pro promoter of the Nopaline synthase gene; NPT II Neomicine phosphotransferase II gene; LB left border; RB right border; LS longitudinal sections; TS transversal sections.

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Section: Discussionmentioning

confidence: 99%

TherolD gene fromAgrobacterium rhizogenes is developmentally regulated in transgenic tobacco

et al. 1997

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“…Tissue excision seems to be the signal which initiates these changes. At least three distinguishable types of aging responses have been recorded: (a) an enhancement of ion uptake requiring up to 24 h for full development, usually accompanied by an increased respiratory rate, which is characteristic of certain storage tissues (27); (b) an enhancement of ion uptake commencing immediately upon excision, accompanied by a decrease or slight increase in respiration rate, which has been reported in barley (9,17) and corn roots (12); and (c) an enhancement of ion accumulation commencing after a predictable several-hour lag, not accompanied by respiratory alterations, such as that reported for pea stem (7,18). The three aging types mentioned above may represent alternative solutions to the same problem: bringing a system into electrochemical steady state with a new environment.…”

Section: Discussionmentioning

confidence: 99%

Electrochemical Aging Responses in Pisum: Cellular Adaptations or Recovery from Injury?

Pierce¹,

Hendrix²

1981

Plant Physiol.

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Following excision, etiolated epicotyl segments of Pisum sativum L. cv. Alaska exhibit a marked hyperpolarization of membrane potential which is followed by a linear accumulation of K' when segments are incubated in Higinbotham nutrient solution. Segments aged for several hours and then reexcised display only a slight depolarization of membrane potential and no delay in ion accumulation; thus, recovery from injury appears an unlikely explanation for these responses. Substances originating in either the plumule or the cotyledons do not seem to be directly involved in these "aging" responses. However, locally produced substances, such as ethylene, or MATERIALS AND METHODSSeeds of Pisum sativum L. cv. Alaska were surface sterilized with 20%o Clorox, soaked for 2 to 4 h in distilled H20 and planted in trays of vermiculite. The plants were grown in the dark for 7 days at 25 C. The upper 1.5 cm ofeach epicotyl was then discarded and the next two 1-cm segments were excised from the third internode. Twenty segments (approximately 0.75 g) were used for each sample in ion uptake experiments. Unless otherwise indicated, excised segments were incubated on a reciprocating shaker in 100 ml of a solution referred to as lx (6). The I x contained, in mM, KCI, 1.0; NaH2PO4, 0.90; Na2HPO4, 0.05; Ca(NO3)2, 1.0 and MgSO4, 0.25. The pH of this solution is approximately 5.4. All uptake and electrical potential measurements were made at 20 C, except where noted.Membrane-bound ATPase was isolated by density gradient centrifugation using linear sucrose gradients (5,21). Gradient fractions with densities corresponding to 30.5 to 32.5% sucrose were pooled and used in these assays (5). ATPase activity was assayed at pH 6.0 or 8.2 and 38 C using a reaction mixture consisting of 3

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“…between the cell vacuole and the external medium increased from about -120 mV to more negative values in the range -160 to -200mV. This hyperpolarization is well established in freshly excised plant tissues and is related to changes in ion transport (Van Steveninck, 1976;Pierce & Hendrix, 1981). There is evidence that such aged preparations are electrophysiologically more representative of intact tissue than arc freshly excised preparations (Starrach et al,, 1984).…”

Section: Electrophysiological Measurementsmentioning

confidence: 96%

Oligosaccharides that induce proteinase inhibitor activity in tomato plants cause depolarization of tomato leaf cells

Thain

Doherty

Bowles

et al. 1990

Plant Cell & Environment

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Abstract. Two size ranges of oligosaccharide elicitors of pectic origin have been investigated for their effects on tomato plants. Both size ranges, with degrees of polymerization of 1–7 and 10–20 respectively, induced the accumulation of proteinase inhibitor (PI) activity in excised plants, and also induced changes in membrane potential of leaf mesophyll cells. The depolarizations were substantial, rapid, and reversible on removal of the elicitors. The effects are discussed in the context of early events in the signal transduction pathway linking oligosaccharides to changes in PI gene expression.

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Cellular Differentiation, Ageing and Ion Transport

Cited by 14 publications

References 211 publications

TherolD gene fromAgrobacterium rhizogenes is developmentally regulated in transgenic tobacco

TherolD gene fromAgrobacterium rhizogenes is developmentally regulated in transgenic tobacco

Electrochemical Aging Responses in Pisum: Cellular Adaptations or Recovery from Injury?

Oligosaccharides that induce proteinase inhibitor activity in tomato plants cause depolarization of tomato leaf cells

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