Cellular death by apoptosis in some radiosensitive and radioresistant mammalian tissues

Suciu, Dan

doi:10.1016/0022-5193(83)90183-2

Cited by 11 publications

(2 citation statements)

References 46 publications

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“…The small intestine is a very radiosensitive tissue [36], so the effect of CIE on the expression of various genes was studied in this tissue. Ionizing radiation can trigger the activation of various pathways such as apoptosis, DNA damage repair and inflammation.…”

Section: Discussionmentioning

confidence: 99%

Mitigation of whole-body gamma radiation–induced damages by Clerodendron infortunatum in mammalian organisms

Chacko¹,

Menon

Majeed

et al. 2016

Journal of Radiation Research

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Several phytoceuticals and extracts of medicinal plants are reported to mitigate deleterious effects of ionizing radiation. The potential of hydro-alcoholic extract of Clerodendron infortunatum (CIE) for providing protection to mice exposed to gamma radiation was investigated. Oral administration of CIE bestowed a survival advantage to mice exposed to lethal doses of gamma radiation. Radiation-induced depletion of the total blood count and bone marrow cellularity were prevented by treatment with CIE. Damage to the cellular DNA (as was evident from the comet assay and the micronucleus index) was also found to be decreased upon CIE administration. Radiation-induced damages to intestinal crypt cells was also reduced by CIE. Studies on gene expression in intestinal cells revealed that there was a marked increase in the Bax/Bcl-2 ratio in mice exposed to whole-body 4 Gy gamma radiation, and that administration of CIE resulted in significant lowering of this ratio, suggestive of reduction of radiation-induced apoptosis. Also, in the intestinal tissue of irradiated animals, following CIE treatment, levels of expression of the DNA repair gene Atm were found to be elevated, and there was reduction in the expression of the inflammatory Cox-2 gene. Thus, our results suggest a beneficial use of Clerodendron infortunatum for mitigating radiation toxicity.

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Section: Discussionmentioning

confidence: 99%

Mitigation of whole-body gamma radiation–induced damages by Clerodendron infortunatum in mammalian organisms

Chacko¹,

Menon

Majeed

et al. 2016

Journal of Radiation Research

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“…Additionally, we introduce the application of a treatment break during radiotherapy to allow for tumor size reduction. Given the lymphoid nature of CD, and the known apoptotic response of lymphoid tissues to radiotherapy32, a reduction of tumor size in CD could reasonably be expected. In our case, we treated the tumor mass to 1620 cGy, then allowed a for 10 day break followed by re-simulation and treatment planning.…”

Section: Discussionmentioning

confidence: 99%

Intensity modulated radiation therapy (IMRT) for the treatment of unicentric Castlemans disease: a case report and review of the use of radiotherapy in the literature

Matthiesen¹,

Ramgopol²,

Seavey³

et al. 2012

Radiology and Oncology

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BackgroundSurgical resection is considered standard therapy for cases of resectable unicentric Castleman’s disease (UCD). Unresectable cases of UCD do not have a consensus regarding the optimal treatment approach, but have utilized steroids, observation, chemotherapy, and radiotherapy. Here we discuss a patient presentation of UCD treated with an advanced radiotherapy technique, IMRT.Case reportA 47 year old female was found to have an intra-thoracic posterior UCD and was determined not to be a good surgical candidate. She was referred for radiotherapy and was treated using IMRT to a total dose of 4320 cGy in 180 cGy fractions including a scheduled 10 day break. Following the break, the patient’s treatment was replanned at which the initial treatment volume was reduced by 50.9% for the duration of the treatment course. Radiation Therapy Oncology Group (RTOG) grade III pneumonitis developed which was managed medically. Neither disease progression nor late effects have occurred.ConclusionsThe use of IMRT and planned treatment break was successful in the treatment of a case of UCD, and should be considered for other unresectable cases.

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Immunohistochemical study of nuclear changes associated with male germ cell death and spermiogenesis

et al. 2009

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In a previous study on the effects of gestational and lactational exposure of para-nonylphenol on male rats, we noted in both induced and uninduced rats, that variations in cleaved caspase-3 immunostaining patterns were associated with distinct nuclear alterations in mainly basally located germ cells (spermatogonia and preleptotene spermatocytes). These were re-analysed and compared with cleaved caspase-3-labeled germ cells in the aging human and the spermatogenically active catfish testis. In the rat testes, cytoplasmic immunostaining was progressively associated with lateral compression of the nucleus, its break up into large pieces which can contain immunostained marginated chromatin masses. The pale remnants of the nucleus continued to shrink in size concomitant with the appearance of blue-purplish stained regions in the cytoplasm similar in color to the condensed chromatin in spermatids, a condition which was TUNEL-negative. These large clumps of chromatin also eventually disappeared, giving rise to cells resembling cytoplasmic ghosts, a condition which was TUNEL-positive. By contrast, the immunolabeled nuclei of human and catfish germ cells condensed into a single mass, after which they lost immunoreactivity. To exclude the possibility that these observations could reflect alterations in Sertoli nuclei, rat testicular sections were probed with a mouse anti-human GATA-4 monoclonal (MHM) antibody. The MHM was, however, the second of two GATA-4 antibodies tested, with a goat anti-mouse polyclonal (GMP) initially used to label the rat Sertoli nuclei. GMP unexpectedly, but distinctly labeled the complete development of the acrosome in the rat testis, a fortuitous finding with utility for staging of the seminiferous epithelium.

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Cellular death by apoptosis in some radiosensitive and radioresistant mammalian tissues

Cited by 11 publications

References 46 publications

Mitigation of whole-body gamma radiation–induced damages by Clerodendron infortunatum in mammalian organisms

Mitigation of whole-body gamma radiation–induced damages by Clerodendron infortunatum in mammalian organisms

Intensity modulated radiation therapy (IMRT) for the treatment of unicentric Castlemans disease: a case report and review of the use of radiotherapy in the literature

Immunohistochemical study of nuclear changes associated with male germ cell death and spermiogenesis

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