Cellular Copper Toxicity: A Critical Appraisal of Fenton-Chemistry-Based Oxidative Stress in Wilson Disease

Zischka, Hans; Lichtmannegger, Josef

doi:10.1016/b978-0-12-811077-5.00006-2

Wilson Disease 2019

DOI: 10.1016/b978-0-12-811077-5.00006-2

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Cellular Copper Toxicity: A Critical Appraisal of Fenton-Chemistry-Based Oxidative Stress in Wilson Disease

Hans Zischka

Josef Lichtmannegger

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2022

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Analysis of copper-induced protein precipitation across the E. coli proteome

et al. 2022

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Metal cations have been exploited for their precipitation properties in a wide variety of studies, ranging from differentiating proteins from serum and blood to identifying the protein targets of drugs. Despite widespread recognition of this phenomenon, the mechanisms of metal-induced protein aggregation have not been fully elucidated. Recent studies have suggested that Cu's ability to induce protein aggregation may be a main contributor to Cu-induced cell death. Here, we provide the first proteome-wide analysis of the relative sensitivities of proteins across the Escherichia coli proteome to Cu-induced aggregation. We utilize a Metal-induced Protein Precipitation methodology that relies on quantitative bottom-up proteomics to define the metal concentration-dependent precipitation properties of proteins on a proteomic scale. Our results establish that Cu far surpasses other metals in promoting protein aggregation and that the protein aggregation is reversible upon metal chelation. The bulk of the Cu bound in the protein aggregates is Cu1+, regardless of the Cu2+ source. Analysis of our Metal-induced Protein Precipitation data allows us to investigate underlying biophysical characteristics that determine a protein's sensitivity to Cu-induced aggregation, which is independent of the relative concentration of protein in the lysate. Overall, this analysis provides new insights into the mechanism behind Cu cytotoxicity, as well as metal cation induced protein aggregation.

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Analysis of copper-induced protein precipitation across the E. coli proteome

et al. 2022

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Cellular Copper Toxicity: A Critical Appraisal of Fenton-Chemistry-Based Oxidative Stress in Wilson Disease

Cited by 1 publication

References 94 publications

Analysis of copper-induced protein precipitation across the E. coli proteome

Analysis of copper-induced protein precipitation across the E. coli proteome

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