Cellular composition of the bone marrow in the chicken. I. identification of cells

Abstract

Incorporation of Fe55 in vivo was used for verifying on radioautographs the identity of chicken bone marrow cells that are in the process of hemesyntheses. Under the experimental conditions all labeled cells may be considered to be linked with erythroid differentiation. They were classified into five maturational stages according to their morphology and capacity for DNA synthesis. Granulocytes were identified by the presence of specific granules. All mononuclear cells were classified as lymphocytes which had a pachychromatic nucleus, a high nucleocytoplasmic ratio, lacked the capacity for DNA synthesis, and resembled small lymphocytes of the bursa, spleen and bone marrow that bound, in vitro, anti-chicken gamma globulins labeled with I125. Radioautography with H3TdR was used to identify proliferating and non-proliferating members of each cell population.

The Effect of 5-Fluorouracil Treatment of Chicks: A Cell Depletion Model for the Study of Avian Polymorphonuclear Leukocytes and Natural Host Defenses

“…The chicken appears to be an ideally suited model for the study of 5-FUmediated granulocytopenia and host resistance because: 1) the chicken does not ppssess a preformed pool of extravascular granulocytes. [PMN must be recruited from the bone marrow constantly (Rose and Hesketh, 1974;Glick and Rosse, 1976;Ficken et al, 1986;Toth and Siegel, 1986)]; and 2) chicken granulocytes mature in the bone marrow from multipotential stem cells in only 6 to 14 h (Glick and Rosse, 1976). Thus, by treating birds with 5-FU, the release of mature PMN into the circulation could effectively be prevented.…”

Characterization of the Pattern of Inflammatory Cell Influx in Chicks Following the Intraperitoneal Administration of Live Salmonella enteritidis and Salmonella enteritidis-Immune Lymphokines

“…To facilitate the study of ILK-induced protection and the inflammatory response in vivo, we made use of the distinctive properties of the chicken peritoneum as a model for inflammatory cell recruitment. Chickens have few resident phagocytic cells in the peritoneal cavity and respiratory tract and, thus, depend upon the constant recruitment of phagocytic cells from the bone marrow (Rose et al, 1974;Glick and Rosse, 1976;Ficken et al, 1986;Toth and Siegel, 1986). This approach is especially suited for studying the regulation of the inflammatory cell responses elicited by both host cell products, including cytokine production, and bacterial cell products during the in vivo host response.…”

Dynamics of the avian inflammatory response toSalmonellafollowing administration of the toll-like receptor 5 agonist flagellin

“…Results from studies of mice parallel the results of FGN administration in the chicken IA model presented here: IA administration of FGN was found to cause an infiltration into the abdominal cavity by heterophils, the avian counterpart to the mammalian neutrophil. Cytokine expression and production were not assayed in the current studies; however, it appears that production of some stimulatory agents takes place in the abdomen, as evidenced by the appearance of leukocytes in what is, under normal conditions, an environment that is free of leukocytes (Rose & Hesketh, 1974; Glick & Rosse, 1976).…”