Cellular cholesterol regulates monocyte deformation

Saha, Amit K.; Dallo, Shatha F.; Detmar, Ariana L.; Osmulski, Paweł A.; Gaczyńska, Maria; Huang, Tim H.M.; Ramasubramanian, Anand K.

doi:10.1016/j.jbiomech.2016.12.033

Cited by 13 publications

(17 citation statements)

References 31 publications

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“…For example, increasing the FC concentration in THP1 cells lead to increased chemotactic response to monocyte chemoattractant protein-1 (MCP-1) due to alterations in C-C motif chemokine receptor 2 (CCR2) levels in lipid rafts. Modulating human monocyte FC affected rolling of these cells on E-selectin-coated surfaces by changing E-selectin counterreceptor CD44 distribution on the lipid rafts suggesting a mechanism through which FC concentration modulates monocyte adhesion by regulation of receptor mobility (7,8).…”

Section: Microdomains and Cellular Cholesterol Homeostasismentioning

confidence: 99%

The ins and outs of lipid rafts: functions in intracellular cholesterol homeostasis, microparticles, and cell membranes

Ouweneel

Thomas

Sorci‐Thomas

2020

Journal of Lipid Research

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Cellular membranes are not homogenous mixtures of proteins; rather, they are segregated into microdomains on the basis of preferential association between specific lipids and proteins. These microdomains, called lipid rafts, are well known for their role in receptor signaling on the plasma membrane (PM) and are essential to such cellular functions as signal transduction and spatial organization of the PM. A number of disease states, including atherosclerosis and other cardiovascular disorders, may be caused by dysfunctional maintenance of lipid rafts. Lipid rafts do not occur only in the PM but also have been found in intracellular membranes and extracellular vesicles (EVs). Here, we focus on discussing newly discovered functions of lipid rafts and microdomains in intracellular membranes, including lipid and protein trafficking from the ER, Golgi bodies, and endosomes to the PM, and we examine lipid raft involvement in the production and composition of EVs. Because lipid rafts are small and transient, visualization remains challenging. Future work with advanced techniques will continue to expand our knowledge about the roles of lipid rafts in cellular functioning.

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Section: Microdomains and Cellular Cholesterol Homeostasismentioning

confidence: 99%

The ins and outs of lipid rafts: functions in intracellular cholesterol homeostasis, microparticles, and cell membranes

Ouweneel

Thomas

Sorci‐Thomas

2020

Journal of Lipid Research

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“…Chol is not only essential for monocyte deformation [ 263 ] but seems also to be involved in their vesiculation, as revealed by the fact that chol depletion decreases MV abundance [ 39 ] whereas chol loading stimulates their release [ 182 ]. These MVs contain TF and PSGL-1, which are both found in DRMs.…”

Section: Microvesicle Biogenesis and Shedding—role Of Plasma Membrmentioning

confidence: 99%

Plasma Membrane Lipid Domains as Platforms for Vesicle Biogenesis and Shedding?

et al. 2018

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Extracellular vesicles (EVs) contribute to several pathophysiological processes and appear as emerging targets for disease diagnosis and therapy. However, successful translation from bench to bedside requires deeper understanding of EVs, in particular their diversity, composition, biogenesis and shedding mechanisms. In this review, we focus on plasma membrane-derived microvesicles (MVs), far less appreciated than exosomes. We integrate documented mechanisms involved in MV biogenesis and shedding, focusing on the red blood cell as a model. We then provide a perspective for the relevance of plasma membrane lipid composition and biophysical properties in microvesiculation on red blood cells but also platelets, immune and nervous cells as well as tumor cells. Although only a few data are available in this respect, most of them appear to converge to the idea that modulation of plasma membrane lipid content, transversal asymmetry and lateral heterogeneity in lipid domains may play a significant role in the vesiculation process. We suggest that lipid domains may represent platforms for inclusion/exclusion of membrane lipids and proteins into MVs and that MVs could originate from distinct domains during physiological processes and disease evolution.

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“…In cells, and most importantly in SM-rich cellular "rafts", cholesterol is likely to be implicated in the regulation of membrane curvature, which is important for many cellular events such as membrane fusion, budding, etc. [74,[78][79][80][81].…”

Section: Role Of Phase State On the Young Modulus Of Milk-sm Bilayersmentioning

confidence: 99%

Young modulus of supported lipid membranes containing milk sphingomyelin in the gel, fluid or liquid-ordered phase, determined using AFM force spectroscopy

Et-Thakafy¹,

Guyomarc’H²,

Lopez³

2019

Biochimica et Biophysica Acta (BBA) - Biomembranes

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The biological membrane surrounding milk fat globules (MFGM) exhibits lateral phase separation of lipids, interpreted as gel or liquid-ordered phase sphingomyelin-rich (milk SM) domains dispersed in a fluid continuous lipid phase. The objective of this study was to investigate whether changes in the phase state of milk SM-rich domains induced by temperature (T < Tm or T > Tm) or cholesterol affected the Young modulus of the lipid membrane. Supported lipid bilayers composed of MFGM polar lipids, milk SM or milk SM/cholesterol (50:50 mol) were investigated at 20°C and 50°C using atomic force microscopy (AFM) and force spectroscopy. At 20°C, gel-phase SM-rich domains and the surrounding fluid phase of the MFGM polar lipids exhibited Young modulus values of 10-20 MPa and 4-6 MPa, respectively. Upon heating at 50°C, the milk SM-rich domains in MFGM bilayers as well as pure milk SM bilayers melted, leading to the formation of a homogeneous membrane with similar Young modulus values to that of a fluid phase (0-5 MPa). Upon addition of cholesterol to the milk SM to reach 50:50 mol%, membranes in the liquid-ordered phase exhibited Young modulus values of a few MPa, at either 20 or 50°C. This indicated that the presence of cholesterol fluidized milk SM membranes and that the Young modulus was weakly affected by the temperature. These results open perspectives for the development of milk polar lipid based vesicles with modulated mechanical properties.

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Cellular cholesterol regulates monocyte deformation

Cited by 13 publications

References 31 publications

The ins and outs of lipid rafts: functions in intracellular cholesterol homeostasis, microparticles, and cell membranes

The ins and outs of lipid rafts: functions in intracellular cholesterol homeostasis, microparticles, and cell membranes

Plasma Membrane Lipid Domains as Platforms for Vesicle Biogenesis and Shedding?

Young modulus of supported lipid membranes containing milk sphingomyelin in the gel, fluid or liquid-ordered phase, determined using AFM force spectroscopy

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