Cellular carbohydrate components in human, rabbit and rat lacrimal gland

Ahmed, Abu; Grierson, Ian

doi:10.1007/bf02169831

Cited by 6 publications

(3 citation statements)

References 32 publications

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“…Lacrimal gland secretions have been considered a source of mucins in the tear film. Evidence for this has not been forthcoming, in spite of histological investigations demonstrating an abundance of carbohydrate reactive material in the gland (Versura et al, 1986;Ahmed and Grierson, 1989;Breipohl et al, 1994). Earlier studies with a polyclonal antibody to semipurified human ocular mucin failed to show any cross-reactivity with the lacrimal gland (Moore and Tiffany, 1979).…”

Section: The Lacrimal Glandsmentioning

confidence: 98%

Ocular mucins: Purification, metabolism and functions

Corfield

Carrington

Hicks

et al. 1997

Progress in Retinal and Eye Research

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Section: The Lacrimal Glandsmentioning

confidence: 98%

Ocular mucins: Purification, metabolism and functions

Corfield

Carrington

Hicks

et al. 1997

Progress in Retinal and Eye Research

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“…Both membrane-associated and secreted mucins have been detected in the lacrimal gland 35,36. Although no specific information is available on O-glycan composition or structure in the human lacrimal gland, lectin histochemistry has shown that the gland contains N-acetylglucosamine, N-acetylgalactosamine, D-galactose, D-mannose, sialic acid and L-fucose 37. Similar lectin experiments have been performed to determine the carbohydrate composition of the lacrimal sac and the nasolacrimal duct—a pseudostratified, columnar epithelia rich in goblet cells—that express membrane-associated and secreted mucins 38.…”

Section: Structure Of Mucin-type O-glycansmentioning

confidence: 99%

Structure and Biological Roles of Mucin-type O-glycans at the Ocular Surface

2010

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Mucins are major components in mucus secretions and apical cell membranes on wet-surfaced epithelia. Structurally, they are characterized by the presence of tandem repeat domains containing heavily O-glycosylated serine and threonine residues. O-glycans contribute to maintaining the highly extended and rigid structure of mucins, conferring to them specific physical and biological properties essential for their protective functions. At the ocular surface epithelia, mucin-type O-glycan chains are short and predominantly sialylated, perhaps reflecting specific requirements of the ocular surface. Traditionally, secreted mucins and their O-glycans in the tear film have been involved in the clearance of debris and pathogens from the surface of the eye. New evidence, however, shows that O-glycans on the cell-surface glycocalyx have additional biological roles in the protection of corneal and conjunctival epithelia, such as preventing bacterial adhesion, promoting boundary lubrication, and maintaining the epithelial barrier function through their interaction with galectin-3. Abnormalities in mucin-type O-glycosylation have been identified in many disorders where the stability of the ocular surface is compromised. This review summarizes recent advances in understanding the structure, biosynthesis, and function of mucin-type O-glycans at the ocular surface and their alteration in ocular surface disease.

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“…Further, in one of the only direct comparisons, tissue distribution of lectins was shown to differ in human LG versus the rat equivalent. 38 These data suggest that although gross structure of the LG is similar between different mammalian species, specific differences can be observed.…”

Section: Introductionmentioning

confidence: 84%

Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering Studies

Wood

Chidlow

Halliday

et al. 2022

Trans. Vis. Sci. Tech.

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Purpose The purpose of this study was to determine whether rodent lacrimal glands (LGs) represent a suitable surrogate for human tissue in bio-engineering research, we undertook a meticulous histological and histochemical comparison of these two tissues. Methods Histological techniques and immunohistochemistry were used to compare the structure of adult human and rat LG tissues and the expression of key functional tissue elements. Results Compared with humans, the rat LG is comprised of much more densely packed acini which are devoid of an obvious central lumen. Myoepithelial, fibroblasts, dendritic cells, T cells, and putative progenitor cells are present in both tissues. However, human LG is replete with epithelium expressing cytokeratins 8 and 18, whereas rat LG epithelium does not express cytokeratin 8. Furthermore, human LG expresses aquaporins (AQPs) 1, 3, and 5, whereas rat LG expresses AQPs 1, 4, and 5. Additionally, mast cells were identified in the rat but not the human LGs and large numbers of plasma cells were detected in the human LGs but only limited numbers were present in the rat LGs. Conclusions The cellular composition of the human and rat LGs is similar, although there is a marked difference in the actual histo-architectural arrangement of the tissue. Further variances in the epithelial cytokeratin profile, in tissue expression of AQPs and in mast cell and plasma cell infiltration, may prove significant. Translational Relevance The rat LG can serve as a useful surrogate for the human equivalent, but there exist specific tissue differences meaning that caution must be observed when translating results to patients.

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Cellular carbohydrate components in human, rabbit and rat lacrimal gland

Cited by 6 publications

References 32 publications

Ocular mucins: Purification, metabolism and functions

Ocular mucins: Purification, metabolism and functions

Structure and Biological Roles of Mucin-type O-glycans at the Ocular Surface

Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering Studies

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