Cellular behaviour of bone marrow stromal cells on modified Ti-Nb surfaces

Abstract

The cellular behaviour of bone marrow stromal cells on titanium surfaces modified by niobium diffusion is presented in order to test their osteogenic differentiation response after culturing for 21 days. The surface modification of Ti substrates produced by powder metallurgy was performed through niobium diffusion treatments. Ti-Nb exhibited a β-Ti surface together with a microstructural (β / α+β / α) and compositional (Ti-Nb) gradient which enhances hardness, wear resistance and lowers the elastic modulus making it more similar to the human bone. Furthermore, the Ti-NbNH4Cl by means of the activating agent achieved three times the hardness of Ti together with a porous surface. The in vitro osteogenic differentiation response of bone marrow stromal cells on both Ti-Nb surfaces indicated the positive cell-material interaction. The osteogenic differentiation of cells was successful after 21 days, considering the positive response in terms of increased cell viability, lactate dehydrogenase-(LDH) activity, alkaline phosphatase-(ALP) activity expression (osteogenic marker) and bone-like nodules deposition by ST-2 cells as a bone mineralization cue. Therefore, the positive effect of a low elastic modulus Ti-Nb surface and a porous nitride Ti-NbNH4Cl with suitable wettability and average roughness values on the osteogenic differentiation response of bone marrow stromal cells is demonstrated.

Fabrication and Characterization of Ag–Sr-Substituted Hydroxyapatite/Chitosan Coatings Deposited via Electrophoretic Deposition: A Design of Experiment Study

“…Similar observations were made in the literature, where it was shown that chitosan/HA coatings increased the surface roughness five times compared to that of the substrate. 45 Recently, Ureña et al 48 showed that the average surface roughness in the range of 1.2–1.5 μm is favorable for the attachment and proliferation of bone marrow stromal cells (ST-2). Therefore, wettability and average roughness of chitosan/AgSr-HA coatings would be better for cells and protein attachment for bone regeneration applications.…”

Ag and Mn-doped mesoporous bioactive glass nanoparticles incorporated into the chitosan/gelatin coatings deposited on PEEK/bioactive glass layers for favorable osteogenic differentiation and antibacterial activity

“…49,50 Furthermore, controlled roughness (R a B 1.3 mm) of the coatings allows the cells to spread over the whole surface. Recently, Ureña et al 51 showed the favorable viability and attachment of ST-2 cells on Ti-Nb surfaces, which were modified to achieve the average roughness in the range of 1.38-1.80 mm. We conclude based on fluorescence microscopy and cell viability test that CGAgMnB coatings persisted and enhanced osteoblastic attachment and proliferation, which could be due to the surface topography, release of ions (dissolution products) from MBGNs and the release of gelatin.…”

Corrosion and tribocorrosion behaviour of β-type Ti-Nb and Ti-Mo surfaces designed by diffusion treatments for biomedical applications

“…Dry sliding wear of conventional biomedical Ti alloys has been the focus of recent studies, nevertheless there is still relatively little knowledge of the corrosion and tribocorrosion properties of diffusionbased powder metallurgy Ti surfaces designed by thermo-chemical treatments. It is worth emphasizing that these Ti-Nb and Ti-Mo surfaces exhibit improved wear resistance [20] and positive cell-material response [21], [22]. Therefore, is also necessary to study corrosion and tribocorrosion behaviour of these materials before considering them for biomaterials.…”