Cellular Based Strategies for Microvascular Engineering

Koduru, Srinivas; Leberfinger, Ashley N.; Pasic, Denis; Forghani, Anoosha; Lince, Shane M.; Hayes, Daniel J.; Özbolat, İbrahim T.; Ravnic, Dino J.

doi:10.1007/s12015-019-09877-4

Cited by 17 publications

(9 citation statements)

References 234 publications

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“…Microvascular ECs can be obtained from skin, lung, heart, or uterine tissues. ECs can also be derived from endothelial progenitor cells (EPCs), which can be isolated from adult peripheral or umbilical cord blood, or derived from pluripotent stem cells (PSCs). − Characteristics of ECs isolated or derived from different tissue sources have been reviewed elsewhere. − …”

Section: The Vascular Endothelium As a Therapeutic Targetmentioning

confidence: 99%

Nucleic Acid Delivery to the Vascular Endothelium

et al. 2022

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This Review examines the state-of-the-art in the delivery of nucleic acid therapies that are directed to the vascular endothelium. First, we review the most important homeostatic functions and properties of the vascular endothelium and summarize the nucleic acid tools that are currently available for gene therapy and nucleic acid delivery. Second, we consider the opportunities available with the endothelium as a therapeutic target and the experimental models that exist to evaluate the potential of those opportunities. Finally, we review the progress to date from investigations that are directly targeting the vascular endothelium: for vascular disease, for peri-transplant therapy, for angiogenic therapies, for pulmonary endothelial disease, and for the blood− brain barrier, ending with a summary of the future outlook in this field.

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Section: The Vascular Endothelium As a Therapeutic Targetmentioning

confidence: 99%

Nucleic Acid Delivery to the Vascular Endothelium

et al. 2022

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“…Compared to the above described cell seeding strategies to generate in vitro preformed microvascular networks, the enzymatic digestion period for the isolation of microvascular fragments is much shorter (5-10 min) than that of single source cells and does not require complex and time-consuming in vitro incubation periods. Moreover, MF can also be obtained from patients in a one-step surgical procedure with a liposuction technique under local anesthesia [124].…”

Section: Microvascular Fragments (Mf)mentioning

confidence: 99%

“…Stem Cells International combination of endothelial cells and stromal cells derived from the SVF assembles into vascular structures, thus actively contributing to the vascularization of tissueengineered bone grafts and stimulating their engraftment in vivo [124].…”

Section: Future Directionsmentioning

confidence: 99%

Bone Tissue Regeneration in the Oral and Maxillofacial Region: A Review on the Application of Stem Cells and New Strategies to Improve Vascularization

Helder

Bravenboer

et al. 2019

Stem Cells International

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Bone tissue engineering techniques are a promising alternative for the use of autologous bone grafts to reconstruct bone defects in the oral and maxillofacial region. However, for successful bone regeneration, adequate vascularization is a prerequisite. This review presents and discusses the application of stem cells and new strategies to improve vascularization, which may lead to feasible clinical applications. Multiple sources of stem cells have been investigated for bone tissue engineering. The stromal vascular fraction (SVF) of human adipose tissue is considered a promising single source for a heterogeneous population of essential cells with, amongst others, osteogenic and angiogenic potential. Enhanced vascularization of tissue-engineered grafts can be achieved by different mechanisms: vascular ingrowth directed from the surrounding host tissue to the implanted graft, vice versa, or concomitantly. Vascular ingrowth into the implanted graft can be enhanced by (i) optimizing the material properties of scaffolds and (ii) their bioactivation by incorporation of growth factors or cell seeding. Vascular ingrowth directed from the implanted graft towards the host tissue can be achieved by incorporating the graft with either (i) preformed microvascular networks or (ii) microvascular fragments (MF). The latter may have stimulating actions on both vascular ingrowth and outgrowth, since they contain angiogenic stem cells like SVF, as well as vascularized matrix fragments. Both adipose tissue-derived SVF and MF are cell sources with clinical feasibility due to their large quantities that can be harvested and applied in a one-step surgical procedure. During the past years, important advancements of stem cell application and vascularization in bone tissue regeneration have been made. The development of engineered in vitro 3D models mimicking the bone defect environment would facilitate new strategies in bone tissue engineering. Successful clinical application requires innovative future investigations enhancing vascularization.

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“…The adequate adhesion of the cells to the scaffold also has an important role in the expression of normal cell functions, such as cell survival, proliferation, and differentiation [17,18]. Above all, porous scaffolds have attracted attention due to their potential to support vascular tube formation [19,20]. However, those studies have not revealed the interaction between the co-cultured osteoblast, endothelial cells, and the porous scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Co-Culture of Osteoblasts and Endothelial Cells on a Microfiber Scaffold to Construct Bone-Like Tissue with Vascular Networks

Inomata

Honda

2019

Materials

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Bone is based on an elaborate system of mineralization and vascularization. In hard tissue engineering, diverse biomaterials compatible with osteogenesis and angiogenesis have been developed. In the present study, to examine the processes of osteogenesis and angiogenesis, osteoblast-like MG-63 cells were co-cultured with human umbilical vein endothelial cells (HUVECs) on a microfiber scaffold. The percentage of adherent cells on the scaffold was more than 60% compared to the culture plate, regardless of the cell type and culture conditions. Cell viability under both monoculture and co-culture conditions was constantly sustained. During the culture periods, the cells were spread along the fibers and extended pseudopodium-like structures on the microfibers three-dimensionally. Compared to the monoculture results, the alkaline phosphatase activity of the co-culture increased 3–6 fold, whereas the vascular endothelial cell growth factor secretion significantly decreased. Immunofluorescent staining of CD31 showed that HUVECs were well spread along the fibers and formed microcapillary-structures. These results suggest that the activation of HUVECs by co-culture with MG-63 could enhance osteoblastic differentiation in the microfiber scaffold, which mimics the microenvironment of the extracellular matrix. This approach can be effective for the construction of tissue-engineered bone with vascular networks.

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Cellular Based Strategies for Microvascular Engineering

Cited by 17 publications

References 234 publications

Nucleic Acid Delivery to the Vascular Endothelium

Nucleic Acid Delivery to the Vascular Endothelium

Bone Tissue Regeneration in the Oral and Maxillofacial Region: A Review on the Application of Stem Cells and New Strategies to Improve Vascularization

Co-Culture of Osteoblasts and Endothelial Cells on a Microfiber Scaffold to Construct Bone-Like Tissue with Vascular Networks

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