Cellular antioxidant activity and Caco‐2 cell uptake characteristics of flavone extracts from Labisia pumila

Abstract

Summary The structures of two extracts of flavone , obtained from the stem (L40) and leaf (S40) of Labisia pumila (LP), were identified through UHPLC‐MS/MS analysis. An 2,2′‐azobis[2‐methylpropionamidine] dihydrochloride (AAPH)‐induced hemolysis model was used and the results indicate the protective effects of flavone extracts on erythrocytes against AAPH‐induced oxidative stress, in which L40 treatment performed the better. Quantitative analysis of variations in antioxidative‐associated enzyme and non‐enzyme systems within the erythrocytes were conducted and led to the identification of protective pathways in which flavone extracts could pass through the cell membrane and act as AAPH‐free radical scavengers. Absorptivity of flavone extracts was characterised using a Caco‐2 monolayer model, in which the apparent permeability coefficient indicated that both types of flavone extracts were readily absorbed compounds, while L40 showed better intestinal absorptivity. Therefore, we conclude that flavonoid extracts from the leaf of LP show remarkable potential functional effects on human health.

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