Cellular antigens in nephroblastoma: Identification with monoclonal antibodies which recognize hemopoietic cells

Platt, Jeffrey L.; Burke, Barbara A.; Michael, Alfred F.

doi:10.1016/0090-1229(87)90162-0

Cited by 5 publications

(1 citation statement)

References 11 publications

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“…The functions of the phenotypic markers initially studied, CD2, CD3, CD4, and CD8, BA-1, among others, were not then understood but since some markers were also expressed in development [8, 9], it seemed that understanding the processes governing the evolution of phenotypes in development would shed light on the function of the markers in mature tissue. It seemed further that changes in the phenotype and function of cells might be governed by glycosaminoglycans, the unique carbohydrate substitutions on proteoglycans, the metabolism of which had been found to drive cell-cell and cell-matrix interactions in development [10].…”

Section: 2 the Immune Response To Transplantationmentioning

confidence: 99%

Heparan Sulfate Proteoglycan Metabolism and the Fate of Grafted Tissues

Platt

Wrenshall

Johnson

et al. 2015

Advances in Experimental Medicine and Biology

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Tissue and organ transplants between genetically distinct individuals are always or nearly always rejected. The universality and speed of transplant rejection distinguishes this immune response from all others. Although this distinction is incompletely understood, some efforts to shed light on transplant rejection have revealed broader insights, including a relationship between activation of complement in grafted tissues, the metabolism of heparan sulfate proteoglycan and the nature of immune and inflammatory responses that ensue. Complement activation on cell surfaces, especially on endothelial cell surfaces, causes the shedding heparan sulfate, an acidic saccharide, from the cell surface and neighboring extracellular matrix. Solubilized in this way, heparan sulfate can activate leukocytes via toll like receptor-4, triggering inflammatory responses and activating dendritic cells, which migrate to regional lymphoid organs where they spark and to some extent govern cellular immune responses. In this way local ischemia, tissue injury and infection, exert systemic impact on immunity. Whether or in what circumstances this series of events explains the distinct characteristics of the immune response to transplants is still unclear but the events offer insight into the inception of immunity under the sub-optimal conditions accompanying infection and mechanisms by which infection and tissue injury engender systemic inflammation.

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Section: 2 the Immune Response To Transplantationmentioning

confidence: 99%

Heparan Sulfate Proteoglycan Metabolism and the Fate of Grafted Tissues

Platt

Wrenshall

Johnson

et al. 2015

Advances in Experimental Medicine and Biology

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Deduction from Wilms' tumour that glomerular podocytes produce the basement membrane material bearing goodpasture determinants

Droz

Diebold

Jan

et al. 1990

The Journal of Pathology

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Using an indirect immunoperoxidase technique, we tested frozen specimens from one Wilms' tumour composed of numerous glomeruloid bodies devoid of blood vessels, with monoclonal antibodies directed against vimentin, cytokeratin, CALLA/CD10, CD24, CR1/CD35, endothelium factor VIII, class I and II MHC molecules, laminin, fibronectin, and non-collagenic domain NC1 of type IV collagen. Two reagents against Goodpasture determinants were used: P1 monoclonal antibody and serum IgG (GP antibodies) from a biopsy-proven Goodpasture patient. Glomeruloid bodies comprised two cell types: a peripheral layer of parietal epithelial cells (cytokeratin and CD24-positive) and central cell clumps of podocytes (vimentin and CALLA-positive). The basal lamina surrounding the glomeruloid bodies contained laminin and NC1 domain of type IV collagen, while that present between the podocytes reacted strongly with laminin, and P1 and GP antibodies. Endothelium factor VIII was not detected within the glomeruloid bodies and CR1 molecules bound to the basement membrane material within them. These data favour the hypothesis that podocytes produce the basement membrane material which bears Goodpasture determinants recently identified as a novel chain, named the alpha 3 chain, of type IV collagen.

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