Cellular and molecular responses of E. fetida cœlomocytes exposed to TiO2 nanoparticles

Bigorgne, Emilie; Foucaud, Laurent; Caillet, Céline; Giambérini, Laure; Nahmani, Johanne; Thomas, Fabien; Rodius, François

doi:10.1007/s11051-012-0959-5

Cited by 16 publications

(20 citation statements)

References 66 publications

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“…For example, some in vitro studies based on various cell lines showed that different TiO 2 NPs cause genotoxicity and cytotoxicity associated with oxidative stress , whereas other studies using human lymphocytes do not show cytotoxicity for similar TiO 2 NPs . For soil invertebrates, an in vitro study using Eisenia fetida coelomocytes showed that TiO 2 NPs were taken up by the cells but with no changes in antioxidative stress levels or cell viability .…”

Section: Introductionmentioning

confidence: 99%

Effect of 10 different TiO₂ and ZrO₂ (nano)materials on the soil invertebrate Enchytraeus crypticus

Gomes

Caputo

Pinna

et al. 2015

Enviro Toxic and Chemistry

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Nearly 80% of all the nano-powders produced worldwide are metal oxides, and among these materials titanium dioxide (TiO2 ) is one of the most produced. Titanium dioxide's toxicity is estimated as low to soil organisms, but some studies have shown that TiO2 nanoparticles can cause oxidative stress. Additionally, it is known that TiO2 is activated by ultraviolet (UV) radiation, which can promote photocatalytic generation of reactive oxygen species, which is seldom taken into account in toxicity testing. In the present study, the authors investigated the effects of different TiO2 and zirconium materials on the soil oligochaete Enchytraeus crypticus, using exposure via soil, water, and soil:water extracts, and studied the effects combined with UV radiation. The results showed that zirconium dioxide (bulk and nano) was not toxic, whereas zirconium tetrachloride reduced enchytraeid reproduction in soil (50% effect concentration = 502 mg/kg). The TiO2 materials were also not toxic via soil exposure or under UV radiation. However, pre-exposure to TiO2 and UV radiation via aqueous media caused a lower reproductive output post-exposure in clean soil (20-50% less but only observed at the lowest concentration tested, 1 mg/L); that is, the effect of TiO2 in water was potentiated by the UV radiation and measurable as a decrease in reproduction in soil media.

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Section: Introductionmentioning

confidence: 99%

Effect of 10 different TiO₂ and ZrO₂ (nano)materials on the soil invertebrate Enchytraeus crypticus

Gomes

Caputo

Pinna

et al. 2015

Enviro Toxic and Chemistry

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“…Previous in vitro studies have shown that NPs can be taken up by earthworm immune cells (coelomocytes) [ 45 , 80 , 81 ], and can alter the expression of earthworm immune markers [ 45 , 46 ], leading to cellular toxicity [ 82 , 83 ]. In this study, in vivo exposure to metal biocides (in both metal salt and NP form) caused changes to microbiome structure, with several bacterial symbionts being negatively affected by exposure to copper.…”

Section: Discussionmentioning

confidence: 99%

The Effects of In Vivo Exposure to Copper Oxide Nanoparticles on the Gut Microbiome, Host Immunity, and Susceptibility to a Bacterial Infection in Earthworms

et al. 2020

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Nanomaterials (NMs) can interact with the innate immunity of organisms. It remains, however, unclear whether these interactions can compromise the immune functioning of the host when faced with a disease threat. Co-exposure with pathogens is thus a powerful approach to assess the immuno-safety of NMs. In this paper, we studied the impacts of in vivo exposure to a biocidal NM on the gut microbiome, host immune responses, and susceptibility of the host to a bacterial challenge in an earthworm. Eisenia fetida were exposed to CuO-nanoparticles in soil for 28 days, after which the earthworms were challenged with the soil bacterium Bacillus subtilis. Immune responses were monitored by measuring mRNA levels of known earthworm immune genes. Effects of treatments on the gut microbiome were also assessed to link microbiome changes to immune responses. Treatments caused a shift in the earthworm gut microbiome. Despite these effects, no impacts of treatment on the expression of earthworm immune markers were recorded. The methodological approach applied in this paper provides a useful framework for improved assessment of immuno-safety of NMs. In addition, we highlight the need to investigate time as a factor in earthworm immune responses to NM exposure.

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“…[11,12,14,94,95] Upon exposure to TiO 2 NP, earthworm engulf NP by phagocytosis, and simultaneously modify mRNA expression of immune proteins and metallothioneins, thereby affecting the molecular response of immune and detoxification systems. [96] Immune cells also express many inducible immune-related genes that are able to bind microbial patterns. Some of these are membrane proteins involved in recognition, e.g., lectins and lectinlike molecules (see previous section), while others are released as soluble proteins, such as FREP and AMP.…”

Section: Nanomaterials and Activation Of Innate Immune Genes/factorsmentioning

confidence: 99%

Addressing Nanomaterial Immunosafety by Evaluating Innate Immunity across Living Species

Boraschi

Alijagić

Auguste

et al. 2020

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The interaction of a living organism with external foreign agents is a central issue for its survival and adaptation to the environment. Nanosafety should be considered within this perspective, and it should be examined that how different organisms interact with engineered nanomaterials (NM) by either mounting a defensive response or by physiologically adapting to them. Herein, the interaction of NM with one of the major biological systems deputed to recognition of and response to foreign challenges, i.e., the immune system, is specifically addressed. The main focus is innate immunity, the only type of immunity in plants, invertebrates, and lower vertebrates, and that coexists with adaptive immunity in higher vertebrates. Because of their presence in the majority of eukaryotic living organisms, innate immune responses can be viewed in a comparative context. In the majority of cases, the interaction of NM with living organisms results in innate immune reactions that eliminate the possible danger with mechanisms that do not lead to damage. While in some cases such interaction may lead to pathological consequences, in some other cases beneficial effects can be identified.

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Cellular and molecular responses of E. fetida cœlomocytes exposed to TiO2 nanoparticles

Cited by 16 publications

References 66 publications

Effect of 10 different TiO₂ and ZrO₂ (nano)materials on the soil invertebrate Enchytraeus crypticus

Effect of 10 different TiO₂ and ZrO₂ (nano)materials on the soil invertebrate Enchytraeus crypticus

The Effects of In Vivo Exposure to Copper Oxide Nanoparticles on the Gut Microbiome, Host Immunity, and Susceptibility to a Bacterial Infection in Earthworms

Addressing Nanomaterial Immunosafety by Evaluating Innate Immunity across Living Species

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Cellular and molecular responses of E. fetida cœlomocytes exposed to TiO2 nanoparticles

Cited by 16 publications

References 66 publications

Effect of 10 different TiO2 and ZrO2 (nano)materials on the soil invertebrate Enchytraeus crypticus

Effect of 10 different TiO2 and ZrO2 (nano)materials on the soil invertebrate Enchytraeus crypticus

The Effects of In Vivo Exposure to Copper Oxide Nanoparticles on the Gut Microbiome, Host Immunity, and Susceptibility to a Bacterial Infection in Earthworms

Addressing Nanomaterial Immunosafety by Evaluating Innate Immunity across Living Species

Contact Info

Product

Resources

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Effect of 10 different TiO₂ and ZrO₂ (nano)materials on the soil invertebrate Enchytraeus crypticus

Effect of 10 different TiO₂ and ZrO₂ (nano)materials on the soil invertebrate Enchytraeus crypticus