Cellular and Molecular Insights Into the Etiology of Subfertility/Infertility in Crossbred Bulls (Bos taurus × Bos indicus): A Review

Kumaresan, A.; Elango, K.; Datta, Tirtha Kumar; Morrell, Jane M.

doi:10.3389/fcell.2021.696637

Cited by 20 publications

(19 citation statements)

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“…Moreover, artificial selection continues to provide an understanding of the basic principles of evolution and natural selection [ 9 ]. A recent review of the etiology of subfertility/infertility in crossbred bulls has highlighted that semen production and sperm quality is lower in crossbred bulls compared to indigenous or purebred bulls [ 41 ]. The difference in sperm quality is ascribed to lower percentages viability, membrane integrity, and well acrosome integrity, which could be related to the downregulation of sperm protein genes [ 41 , 42 ].…”

Section: Which Semen and Sperm Traits Have Been Recorded?mentioning

confidence: 99%

“…A recent review of the etiology of subfertility/infertility in crossbred bulls has highlighted that semen production and sperm quality is lower in crossbred bulls compared to indigenous or purebred bulls [ 41 ]. The difference in sperm quality is ascribed to lower percentages viability, membrane integrity, and well acrosome integrity, which could be related to the downregulation of sperm protein genes [ 41 , 42 ]. In addition, similar studies involving more sperm functional markers (proteomics and metabolomics) may assist in providing better sensitivity in terms of differences in sperm functionality among indigenous-, exotic- and crossbreeds.…”

Section: Which Semen and Sperm Traits Have Been Recorded?mentioning

confidence: 99%

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Origin, Migration, and Reproduction of Indigenous Domestic Animals with Special Reference to Their Sperm Quality

Horst

Maree

2022

Animals

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Indigenous domestic animals such as cattle, sheep, goats, pigs, and chickens have a natural resistance to endo- and ecto-parasites and are tolerant in terms of harsh environmental conditions. These species orginated from the Fertile Cresent between 12,000 and 10,000 BP before migrating into surrounding continents. In view of limited information on the reproductive status of indigenous breeds, it is important to examine their semen characteristics in order to select males to improve livestock production. We have largely relied on existing literature but also our published and ongoing research on sperm quality assessment of several indigenous breeds. The sperm quality of these breeds is similar to current commercial breeds and has been quantified using cutting-edge methods. In this context, we have presented sperm functional tests which provide a better estimate of semen quality than just a standard semen analysis. Initial results suggest that the indigenous breeds have a high sperm quality and sperm functionality similar to currently farmed exotic or crossbreeds. In the long-term, the importance of preserving the favorable traits of these breeds is a priority in view of crossbreeding with existing good meat and milk producers.

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Section: Which Semen and Sperm Traits Have Been Recorded?mentioning

confidence: 99%

Section: Which Semen and Sperm Traits Have Been Recorded?mentioning

confidence: 99%

Origin, Migration, and Reproduction of Indigenous Domestic Animals with Special Reference to Their Sperm Quality

Horst

Maree

2022

Animals

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“…Although AI of native cows with cryopreserved spermatozoa from high genetic exotic bulls/crossbred bulls improved the milk production substantially, the coverage of AI is limited in these countries. One of the major reasons for the low coverage of AI is the limited availability of quality semen ( 1 ). Generally, breeding bulls are selected based on their genetic merit and their ability to fulfill the requirements of breeding soundness evaluation (BSE).…”

Section: Introductionmentioning

confidence: 99%

Sperm Transcripts Associated With Odorant Binding and Olfactory Transduction Pathways Are Altered in Breeding Bulls Producing Poor-Quality Semen

et al. 2022

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Spermatozoa carries a reservoir of mRNAs regulating sperm functions and fertilizing potential. Although it is well recognized that a considerable proportion of high genetic merit breeding bulls produce poor-quality semen, the transcriptomic alterations in spermatozoa from such bulls are not understood. In the present study, comparative high-throughput transcriptomic profiling of spermatozoa from good and poor-quality semen-producing bulls was carried out to identify the transcripts associated with semen quality. Using next-generation sequencing (NGS), we identified 11,632 transcripts in Holstein Friesian bull spermatozoa; after total hit normalization, a total of 544 transcripts were detected, of which 185 transcripts were common to both good and poor-quality semen, while 181 sperm transcripts were unique to good quality semen, and 178 transcripts were unique to poor-quality semen. Among the co-expressed transcripts, 31 were upregulated, while 108 were downregulated, and 46 were neutrally expressed in poor-quality semen. Bioinformatics analysis revealed that the dysregulated transcripts were predominantly involved in molecular function, such as olfactory receptor activity and odor binding, and in biological process, such as detection of chemical stimulus involved in sensory perception, sensory perception of smell, signal transduction, and signal synaptic transmission. Since a majority of the dysregulated transcripts were involved in the olfactory pathway (85% of enriched dysregulated genes were involved in this pathway), the expression of selected five transcripts associated with this pathway (OR2T11, OR10S1, ORIL3, OR5M11, and PRRX1) were validated using real-time qPCR, and it was found that their transcriptional abundance followed the same trend as observed in NGS; the sperm transcriptional abundance of OR2T11 and OR10S1 differed significantly (p < 0.05) between good and poor-quality semen. It is concluded that poor-quality semen showed altered expression of transcripts associated with olfactory receptors and pathways indicating the relationship between olfactory pathway and semen quality in bulls.

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“…Despite great efforts to evaluate bulls using BSE, bull fertility is deemed suboptimal under eld conditions, with a conception rate varying from 20 to 40 % (Kumaresan et al 2021). Such differences may be due to subtle sperm subpopulations and sperm abnormalities that might be overlooked using current, established techniques.…”

Section: Introductionmentioning

confidence: 99%

SNPs Cumulating to Genetic Variation for Fertility in Crossbred (Bos taurus X Bos indicus) Bull Spermatozoa

Sinha

Kumaresan

King

et al. 2022

Preprint

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Purpose: The objective of the present study is to identify important polymorphisms at single nucleotide in Holstein crossbred bull spermatozoa and to identify genetic variants significantly influence or associated to bull fertility. Methods: Spermatozoa from high- (HF) and low-fertile (LF) breeding bulls were subjected to high throughput Next-generation sequencing to identify important SNPs and novel variants associated with fertility.Results: A total of 77,036 genome wide SNPs were identified in the crossbred bull spermatozoa with a minimum read depth of 20, and among them, 10,269 were observed to be novel variants. A total of 42,290 and 34,748 variants were recorded in HF and LF bulls respectively, among which 6115 and 4673 were found to novel in respective group. Higher number of SNPs and Indels were identified in HF compared to LF bulls. GO analysis of filtered genes with significant variation in HF bulls indicated their enrichment in various important pathways like oxidative phosphorylation and metabolic pathways. On the other hand, GO analysis of filtered genes with significant variation in LF bulls revealed their involvement in Ca2++ ion binding, structural constituent of ribosome, and biological processes like translation and ribosomal small subunit assembly. Conclusions: The study identified SNPs in candidate genes including TPT1, BOLA-DRA, CD74, RPS17, RPS28, RPS29, RPL14, RPL13 and RPS27A, which are linked to sperm functionality, survival, protection from oxidative stress and bull fertility. The identified SNPs could be used as a tool for identification and selection of bulls for high fertility upon validation in large number of bulls

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Cellular and Molecular Insights Into the Etiology of Subfertility/Infertility in Crossbred Bulls (Bos taurus × Bos indicus): A Review

Cited by 20 publications

References 91 publications

Origin, Migration, and Reproduction of Indigenous Domestic Animals with Special Reference to Their Sperm Quality

Origin, Migration, and Reproduction of Indigenous Domestic Animals with Special Reference to Their Sperm Quality

Sperm Transcripts Associated With Odorant Binding and Olfactory Transduction Pathways Are Altered in Breeding Bulls Producing Poor-Quality Semen

SNPs Cumulating to Genetic Variation for Fertility in Crossbred (Bos taurus X Bos indicus) Bull Spermatozoa

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